Extracellular Vesicles’ Genetic Cargo as Noninvasive Biomarkers in Cancer: A Pilot Study Using ExoGAG Technology
The two most developed biomarkers in liquid biopsy (LB)—circulating tumor cells and circulating tumor DNA—have been joined by the analysis of extracellular vesicles (EVs). EVs are lipid-bilayer enclosed structures released by all cell types containing a variety of molecules, including DNA, mRNA and...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/2/404 |
| _version_ | 1827758468450222080 |
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| author | Carolina Herrero Alba Ferreirós Daniel Pérez-Fentes Luis León-Mateos Rafael López-López Miguel Abal Lorena Alonso-Alconada |
| author_facet | Carolina Herrero Alba Ferreirós Daniel Pérez-Fentes Luis León-Mateos Rafael López-López Miguel Abal Lorena Alonso-Alconada |
| author_sort | Carolina Herrero |
| collection | DOAJ |
| description | The two most developed biomarkers in liquid biopsy (LB)—circulating tumor cells and circulating tumor DNA—have been joined by the analysis of extracellular vesicles (EVs). EVs are lipid-bilayer enclosed structures released by all cell types containing a variety of molecules, including DNA, mRNA and miRNA. However, fast, efficient and a high degree of purity isolation technologies are necessary for their clinical routine implementation. In this work, the use of ExoGAG, a new easy-to-use EV isolation technology, was validated for the isolation of EVs from plasma and urine samples. After demonstrating its efficiency, an analysis of the genetic material contained in the EVs was carried out. Firstly, the sensitivity of the detection of point mutations in DNA from plasma EVs isolated by ExoGAG was analyzed. Then, a pilot study of mRNA expression using the nCounter NanoString platform in EV-mRNA from a healthy donor, a benign prostate hyperplasia patient and metastatic prostate cancer patient plasma and urine samples was performed, identifying the prostate cancer pathway as one of the main ones. This work provides evidence for the value of using ExoGAG for the isolation of EVs from plasma and urine samples, enabling downstream applications of the analysis of their genetic cargo. |
| first_indexed | 2024-03-11T09:06:47Z |
| format | Article |
| id | doaj.art-3ffe0dfdbacd4a2f8c3f1bef5ec4e533 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-11T09:06:47Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-3ffe0dfdbacd4a2f8c3f1bef5ec4e5332023-11-16T19:17:44ZengMDPI AGBiomedicines2227-90592023-01-0111240410.3390/biomedicines11020404Extracellular Vesicles’ Genetic Cargo as Noninvasive Biomarkers in Cancer: A Pilot Study Using ExoGAG TechnologyCarolina Herrero0Alba Ferreirós1Daniel Pérez-Fentes2Luis León-Mateos3Rafael López-López4Miguel Abal5Lorena Alonso-Alconada6Translational Medical Oncology Group (Oncomet), Health Research Institute of Santiago de Compostela (IDIS), University Hospital of Santiago de Compostela (SERGAS), CIBERONC, 15706 Santiago de Compostela, SpainNasasbiotech, S.L., Canton Grande 9, 15003 A Coruña, SpainUrology Department, University Hospital of Santiago de Compostela (SERGAS), 15706 Santiago de Compostela, SpainTranslational Medical Oncology Group (Oncomet), Health Research Institute of Santiago de Compostela (IDIS), University Hospital of Santiago de Compostela (SERGAS), CIBERONC, 15706 Santiago de Compostela, SpainTranslational Medical Oncology Group (Oncomet), Health Research Institute of Santiago de Compostela (IDIS), University Hospital of Santiago de Compostela (SERGAS), CIBERONC, 15706 Santiago de Compostela, SpainTranslational Medical Oncology Group (Oncomet), Health Research Institute of Santiago de Compostela (IDIS), University Hospital of Santiago de Compostela (SERGAS), CIBERONC, 15706 Santiago de Compostela, SpainNasasbiotech, S.L., Canton Grande 9, 15003 A Coruña, SpainThe two most developed biomarkers in liquid biopsy (LB)—circulating tumor cells and circulating tumor DNA—have been joined by the analysis of extracellular vesicles (EVs). EVs are lipid-bilayer enclosed structures released by all cell types containing a variety of molecules, including DNA, mRNA and miRNA. However, fast, efficient and a high degree of purity isolation technologies are necessary for their clinical routine implementation. In this work, the use of ExoGAG, a new easy-to-use EV isolation technology, was validated for the isolation of EVs from plasma and urine samples. After demonstrating its efficiency, an analysis of the genetic material contained in the EVs was carried out. Firstly, the sensitivity of the detection of point mutations in DNA from plasma EVs isolated by ExoGAG was analyzed. Then, a pilot study of mRNA expression using the nCounter NanoString platform in EV-mRNA from a healthy donor, a benign prostate hyperplasia patient and metastatic prostate cancer patient plasma and urine samples was performed, identifying the prostate cancer pathway as one of the main ones. This work provides evidence for the value of using ExoGAG for the isolation of EVs from plasma and urine samples, enabling downstream applications of the analysis of their genetic cargo.https://www.mdpi.com/2227-9059/11/2/404extracellular vesiclesExoGAGEV-DNAEV-mRNAprostate cancer |
| spellingShingle | Carolina Herrero Alba Ferreirós Daniel Pérez-Fentes Luis León-Mateos Rafael López-López Miguel Abal Lorena Alonso-Alconada Extracellular Vesicles’ Genetic Cargo as Noninvasive Biomarkers in Cancer: A Pilot Study Using ExoGAG Technology Biomedicines extracellular vesicles ExoGAG EV-DNA EV-mRNA prostate cancer |
| title | Extracellular Vesicles’ Genetic Cargo as Noninvasive Biomarkers in Cancer: A Pilot Study Using ExoGAG Technology |
| title_full | Extracellular Vesicles’ Genetic Cargo as Noninvasive Biomarkers in Cancer: A Pilot Study Using ExoGAG Technology |
| title_fullStr | Extracellular Vesicles’ Genetic Cargo as Noninvasive Biomarkers in Cancer: A Pilot Study Using ExoGAG Technology |
| title_full_unstemmed | Extracellular Vesicles’ Genetic Cargo as Noninvasive Biomarkers in Cancer: A Pilot Study Using ExoGAG Technology |
| title_short | Extracellular Vesicles’ Genetic Cargo as Noninvasive Biomarkers in Cancer: A Pilot Study Using ExoGAG Technology |
| title_sort | extracellular vesicles genetic cargo as noninvasive biomarkers in cancer a pilot study using exogag technology |
| topic | extracellular vesicles ExoGAG EV-DNA EV-mRNA prostate cancer |
| url | https://www.mdpi.com/2227-9059/11/2/404 |
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