An Engineered Mouse to Identify Proliferating Cells and Their Derivatives
BackgroundCell proliferation is a fundamental event during development, disease, and regeneration. Effectively tracking and quantifying proliferating cells and their derivatives is critical for addressing many research questions. Cell cycle expression such as for Ki67, proliferating cell nuclear ant...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-05-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fcell.2020.00388/full |
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| author | Jihyun Jang Kurt A. Engleka Kurt A. Engleka Feiyan Liu Feiyan Liu Li Li Guang Song Jonathan A. Epstein Jonathan A. Epstein Deqiang Li |
| author_facet | Jihyun Jang Kurt A. Engleka Kurt A. Engleka Feiyan Liu Feiyan Liu Li Li Guang Song Jonathan A. Epstein Jonathan A. Epstein Deqiang Li |
| author_sort | Jihyun Jang |
| collection | DOAJ |
| description | BackgroundCell proliferation is a fundamental event during development, disease, and regeneration. Effectively tracking and quantifying proliferating cells and their derivatives is critical for addressing many research questions. Cell cycle expression such as for Ki67, proliferating cell nuclear antigen (PCNA), or aurora kinase B (Aurkb), or measurement of 5-bromo-2′-deoxyuridine (BrdU) or 3H-thymidine incorporation have been widely used to assess and quantify cell proliferation. These are powerful tools for detecting actively proliferating cells, but they do not identify cell populations derived from proliferating progenitors over time.AimsWe developed a new mouse tool for lineage tracing of proliferating cells by targeting the Aurkb allele.ResultsIn quiescent cells or cells arrested at G1/S, little or no Aurkb mRNA is detectable. In cycling cells, Aurkb transcripts are detectable at G2 and become undetectable by telophase. These findings suggest that Aurkb transcription is restricted to proliferating cells and is tightly coupled to cell proliferation. Accordingly, we generated an AurkbER Cre/+ mouse by targeting a tamoxifen inducible Cre cassette into the start codon of Aurkb. We find that the AurkbER Cre/+ mouse faithfully labels proliferating cells in developing embryos and regenerative adult tissues such as intestine but does not label quiescent cells such as post-mitotic neurons.ConclusionThe AurkbER Cre/+ mouse faithfully labels proliferating cells and their derivatives in developing embryos and regenerative adult tissues. This new mouse tool provides a novel genetic tracing capability for studying tissue proliferation and regeneration. |
| first_indexed | 2024-12-14T11:59:29Z |
| format | Article |
| id | doaj.art-3ffe2d65f17445419c29decd5ee4a9d0 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-14T11:59:29Z |
| publishDate | 2020-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-3ffe2d65f17445419c29decd5ee4a9d02022-12-21T23:02:01ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2020-05-01810.3389/fcell.2020.00388535216An Engineered Mouse to Identify Proliferating Cells and Their DerivativesJihyun Jang0Kurt A. Engleka1Kurt A. Engleka2Feiyan Liu3Feiyan Liu4Li Li5Guang Song6Jonathan A. Epstein7Jonathan A. Epstein8Deqiang Li9Department of Surgery, Center for Vascular and Inflammatory Diseases, University of Maryland School of Medicine, Baltimore, MD, United StatesDepartment of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United StatesPenn Cardiovascular Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United StatesDepartment of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United StatesPenn Cardiovascular Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United StatesPenn Cardiovascular Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United StatesDepartment of Surgery, Center for Vascular and Inflammatory Diseases, University of Maryland School of Medicine, Baltimore, MD, United StatesDepartment of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United StatesPenn Cardiovascular Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United StatesDepartment of Surgery, Center for Vascular and Inflammatory Diseases, University of Maryland School of Medicine, Baltimore, MD, United StatesBackgroundCell proliferation is a fundamental event during development, disease, and regeneration. Effectively tracking and quantifying proliferating cells and their derivatives is critical for addressing many research questions. Cell cycle expression such as for Ki67, proliferating cell nuclear antigen (PCNA), or aurora kinase B (Aurkb), or measurement of 5-bromo-2′-deoxyuridine (BrdU) or 3H-thymidine incorporation have been widely used to assess and quantify cell proliferation. These are powerful tools for detecting actively proliferating cells, but they do not identify cell populations derived from proliferating progenitors over time.AimsWe developed a new mouse tool for lineage tracing of proliferating cells by targeting the Aurkb allele.ResultsIn quiescent cells or cells arrested at G1/S, little or no Aurkb mRNA is detectable. In cycling cells, Aurkb transcripts are detectable at G2 and become undetectable by telophase. These findings suggest that Aurkb transcription is restricted to proliferating cells and is tightly coupled to cell proliferation. Accordingly, we generated an AurkbER Cre/+ mouse by targeting a tamoxifen inducible Cre cassette into the start codon of Aurkb. We find that the AurkbER Cre/+ mouse faithfully labels proliferating cells in developing embryos and regenerative adult tissues such as intestine but does not label quiescent cells such as post-mitotic neurons.ConclusionThe AurkbER Cre/+ mouse faithfully labels proliferating cells and their derivatives in developing embryos and regenerative adult tissues. This new mouse tool provides a novel genetic tracing capability for studying tissue proliferation and regeneration.https://www.frontiersin.org/article/10.3389/fcell.2020.00388/fullcell proliferationaurora kinase Bmouse modellineage tracingregenerationdevelopment |
| spellingShingle | Jihyun Jang Kurt A. Engleka Kurt A. Engleka Feiyan Liu Feiyan Liu Li Li Guang Song Jonathan A. Epstein Jonathan A. Epstein Deqiang Li An Engineered Mouse to Identify Proliferating Cells and Their Derivatives Frontiers in Cell and Developmental Biology cell proliferation aurora kinase B mouse model lineage tracing regeneration development |
| title | An Engineered Mouse to Identify Proliferating Cells and Their Derivatives |
| title_full | An Engineered Mouse to Identify Proliferating Cells and Their Derivatives |
| title_fullStr | An Engineered Mouse to Identify Proliferating Cells and Their Derivatives |
| title_full_unstemmed | An Engineered Mouse to Identify Proliferating Cells and Their Derivatives |
| title_short | An Engineered Mouse to Identify Proliferating Cells and Their Derivatives |
| title_sort | engineered mouse to identify proliferating cells and their derivatives |
| topic | cell proliferation aurora kinase B mouse model lineage tracing regeneration development |
| url | https://www.frontiersin.org/article/10.3389/fcell.2020.00388/full |
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