Ketogenic diet preserves muscle mass and strength in a mouse model of type 2 diabetes.
Diabetes is often associated with reduced muscle mass and function. The ketogenic diet (KD) may improve muscle mass and function via the induction of nutritional ketosis. To test whether the KD is able to preserve muscle mass and strength in a mouse model of type 2 diabetes (T2DM), C57BL/6J mice wer...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2024-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0296651&type=printable |
| _version_ | 1827381622780985344 |
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| author | Sol Been Park Soo Jin Yang |
| author_facet | Sol Been Park Soo Jin Yang |
| author_sort | Sol Been Park |
| collection | DOAJ |
| description | Diabetes is often associated with reduced muscle mass and function. The ketogenic diet (KD) may improve muscle mass and function via the induction of nutritional ketosis. To test whether the KD is able to preserve muscle mass and strength in a mouse model of type 2 diabetes (T2DM), C57BL/6J mice were assigned to lean control, diabetes control, and KD groups. The mice were fed a standard diet (10% kcal from fat) or a high-fat diet (HFD) (60% kcal from fat). The diabetic condition was induced by a single injection of streptozotocin (STZ; 100 mg/kg) and nicotinamide (NAM; 120 mg/kg) into HFD-fed mice. After 8-week HFD feeding, the KD (90% kcal from fat) was fed to the KD group for the following 6 weeks. After the 14-week experimental period, an oral glucose tolerance test and grip strength test were conducted. Type 2 diabetic condition induced by HFD feeding and STZ/NAM injection resulted in reduced muscle mass and grip strength, and smaller muscle fiber areas. The KD nutritional intervention improved these effects. Additionally, the KD altered the gene expression of nucleotide-binding and oligomerization domain-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome- and endoplasmic reticulum (ER) stress-related markers in the muscles of diabetic mice. Collectively, KD improved muscle mass and function with alterations in NLRP3 inflammasome and ER stress. |
| first_indexed | 2024-03-08T14:06:54Z |
| format | Article |
| id | doaj.art-3fff1b44f2784f089398fbc41de650ef |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-03-08T14:06:54Z |
| publishDate | 2024-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-3fff1b44f2784f089398fbc41de650ef2024-01-15T05:31:29ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-01191e029665110.1371/journal.pone.0296651Ketogenic diet preserves muscle mass and strength in a mouse model of type 2 diabetes.Sol Been ParkSoo Jin YangDiabetes is often associated with reduced muscle mass and function. The ketogenic diet (KD) may improve muscle mass and function via the induction of nutritional ketosis. To test whether the KD is able to preserve muscle mass and strength in a mouse model of type 2 diabetes (T2DM), C57BL/6J mice were assigned to lean control, diabetes control, and KD groups. The mice were fed a standard diet (10% kcal from fat) or a high-fat diet (HFD) (60% kcal from fat). The diabetic condition was induced by a single injection of streptozotocin (STZ; 100 mg/kg) and nicotinamide (NAM; 120 mg/kg) into HFD-fed mice. After 8-week HFD feeding, the KD (90% kcal from fat) was fed to the KD group for the following 6 weeks. After the 14-week experimental period, an oral glucose tolerance test and grip strength test were conducted. Type 2 diabetic condition induced by HFD feeding and STZ/NAM injection resulted in reduced muscle mass and grip strength, and smaller muscle fiber areas. The KD nutritional intervention improved these effects. Additionally, the KD altered the gene expression of nucleotide-binding and oligomerization domain-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome- and endoplasmic reticulum (ER) stress-related markers in the muscles of diabetic mice. Collectively, KD improved muscle mass and function with alterations in NLRP3 inflammasome and ER stress.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0296651&type=printable |
| spellingShingle | Sol Been Park Soo Jin Yang Ketogenic diet preserves muscle mass and strength in a mouse model of type 2 diabetes. PLoS ONE |
| title | Ketogenic diet preserves muscle mass and strength in a mouse model of type 2 diabetes. |
| title_full | Ketogenic diet preserves muscle mass and strength in a mouse model of type 2 diabetes. |
| title_fullStr | Ketogenic diet preserves muscle mass and strength in a mouse model of type 2 diabetes. |
| title_full_unstemmed | Ketogenic diet preserves muscle mass and strength in a mouse model of type 2 diabetes. |
| title_short | Ketogenic diet preserves muscle mass and strength in a mouse model of type 2 diabetes. |
| title_sort | ketogenic diet preserves muscle mass and strength in a mouse model of type 2 diabetes |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0296651&type=printable |
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