Source, Modification, Heterologous Expression of β-Galactosidase and Its Application in Food
β-galactosidase, as a safe and nontoxic enzyme preparation, has been not only widely used in food industry and medical fields, but also has great application potential in biotechnology fields, such as enzyme engineering and protein engineering. Microbial fermentation, as a mainstream production meth...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
The editorial department of Science and Technology of Food Industry
2023-12-01
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| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023030097 |
| _version_ | 1797448797662478336 |
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| author | Yunliang LI Pengfei XIE Xiaoshuang LIU Jiapin CHAO Xiaoxue ZHAO Xiaojing WANG Haile MA |
| author_facet | Yunliang LI Pengfei XIE Xiaoshuang LIU Jiapin CHAO Xiaoxue ZHAO Xiaojing WANG Haile MA |
| author_sort | Yunliang LI |
| collection | DOAJ |
| description | β-galactosidase, as a safe and nontoxic enzyme preparation, has been not only widely used in food industry and medical fields, but also has great application potential in biotechnology fields, such as enzyme engineering and protein engineering. Microbial fermentation, as a mainstream production method of β-galactosidase, still has some problems including long fermentation time and low extraction rate. While using the heterologous expression system of engineering bacteria to produce β-galactosidase shows the advantages of high expression quantity and low cost. This paper focuses on the gene source, expression host bacteria, expression methods of β-galactosidase heterologous expression system and its application value, to be aimed at providing scientific basis and theoretical reference for the development and utilization of novel β-galactosidase products. |
| first_indexed | 2024-03-09T14:15:38Z |
| format | Article |
| id | doaj.art-3fffbd46c2dc465ba80094c72792489a |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-03-09T14:15:38Z |
| publishDate | 2023-12-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-3fffbd46c2dc465ba80094c72792489a2023-11-29T01:58:16ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-12-01442338739310.13386/j.issn1002-0306.20230300972023030097-23Source, Modification, Heterologous Expression of β-Galactosidase and Its Application in FoodYunliang LI0Pengfei XIE1Xiaoshuang LIU2Jiapin CHAO3Xiaoxue ZHAO4Xiaojing WANG5Haile MA6School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212000, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212000, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212000, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212000, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212000, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212000, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212000, Chinaβ-galactosidase, as a safe and nontoxic enzyme preparation, has been not only widely used in food industry and medical fields, but also has great application potential in biotechnology fields, such as enzyme engineering and protein engineering. Microbial fermentation, as a mainstream production method of β-galactosidase, still has some problems including long fermentation time and low extraction rate. While using the heterologous expression system of engineering bacteria to produce β-galactosidase shows the advantages of high expression quantity and low cost. This paper focuses on the gene source, expression host bacteria, expression methods of β-galactosidase heterologous expression system and its application value, to be aimed at providing scientific basis and theoretical reference for the development and utilization of novel β-galactosidase products.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023030097β-galactosidaseapplication valuegene modificationimmobilizationheterologous expression |
| spellingShingle | Yunliang LI Pengfei XIE Xiaoshuang LIU Jiapin CHAO Xiaoxue ZHAO Xiaojing WANG Haile MA Source, Modification, Heterologous Expression of β-Galactosidase and Its Application in Food Shipin gongye ke-ji β-galactosidase application value gene modification immobilization heterologous expression |
| title | Source, Modification, Heterologous Expression of β-Galactosidase and Its Application in Food |
| title_full | Source, Modification, Heterologous Expression of β-Galactosidase and Its Application in Food |
| title_fullStr | Source, Modification, Heterologous Expression of β-Galactosidase and Its Application in Food |
| title_full_unstemmed | Source, Modification, Heterologous Expression of β-Galactosidase and Its Application in Food |
| title_short | Source, Modification, Heterologous Expression of β-Galactosidase and Its Application in Food |
| title_sort | source modification heterologous expression of β galactosidase and its application in food |
| topic | β-galactosidase application value gene modification immobilization heterologous expression |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023030097 |
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