Label-free-based proteomic analysis reveals differential whey proteins of porcine milk during lactation
In this study, label-free proteomic technology was applied to analyze and compare the whey proteomes of porcine colostrum and mature milk. In total, 2993 and 2906 whey proteins were detected in porcine colostrum and mature milk, respectively. A total of 2745 common proteins were identified in the tw...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-03-01
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| Series: | Food Chemistry: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157523005552 |
| _version_ | 1797244904134410240 |
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| author | Huiwen Zhao Shanshan Zhao Qing Zhu Jiali Chen Zhizhong Quan Xiqing Yue Xueyan Cao |
| author_facet | Huiwen Zhao Shanshan Zhao Qing Zhu Jiali Chen Zhizhong Quan Xiqing Yue Xueyan Cao |
| author_sort | Huiwen Zhao |
| collection | DOAJ |
| description | In this study, label-free proteomic technology was applied to analyze and compare the whey proteomes of porcine colostrum and mature milk. In total, 2993 and 2906 whey proteins were detected in porcine colostrum and mature milk, respectively. A total of 2745 common proteins were identified in the two milk samples, and 280 proteins were found to be significantly differentially expressed whey proteins in porcine milk. Gene Ontology analysis demonstrated that the differentially expressed whey proteins were primarily enriched in lipid homeostasis, oxidoreductase activity, and the collagen trimer. Kyoto Encyclopedia of Genes and Genomes analysis suggested that the phagosome and endocytosis were the crucial pathways. This study provides systematic and in-depth insight into the compositions and functional properties of whey proteins in porcine milk during different periods of lactation, which may be beneficial for the development of porcine whey proteins in the future. |
| first_indexed | 2024-03-08T16:50:54Z |
| format | Article |
| id | doaj.art-40013bf8cd4b4215871266b95059f78d |
| institution | Directory Open Access Journal |
| issn | 2590-1575 |
| language | English |
| last_indexed | 2024-04-24T19:18:25Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj.art-40013bf8cd4b4215871266b95059f78d2024-03-26T04:27:13ZengElsevierFood Chemistry: X2590-15752024-03-0121101112Label-free-based proteomic analysis reveals differential whey proteins of porcine milk during lactationHuiwen Zhao0Shanshan Zhao1Qing Zhu2Jiali Chen3Zhizhong Quan4Xiqing Yue5Xueyan Cao6College of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaLiaoning Complete Biotechnology Co., Ltd., Tieling 112600, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, China; Corresponding author.In this study, label-free proteomic technology was applied to analyze and compare the whey proteomes of porcine colostrum and mature milk. In total, 2993 and 2906 whey proteins were detected in porcine colostrum and mature milk, respectively. A total of 2745 common proteins were identified in the two milk samples, and 280 proteins were found to be significantly differentially expressed whey proteins in porcine milk. Gene Ontology analysis demonstrated that the differentially expressed whey proteins were primarily enriched in lipid homeostasis, oxidoreductase activity, and the collagen trimer. Kyoto Encyclopedia of Genes and Genomes analysis suggested that the phagosome and endocytosis were the crucial pathways. This study provides systematic and in-depth insight into the compositions and functional properties of whey proteins in porcine milk during different periods of lactation, which may be beneficial for the development of porcine whey proteins in the future.http://www.sciencedirect.com/science/article/pii/S2590157523005552Porcine milkProteomicsWhey proteinsLactation |
| spellingShingle | Huiwen Zhao Shanshan Zhao Qing Zhu Jiali Chen Zhizhong Quan Xiqing Yue Xueyan Cao Label-free-based proteomic analysis reveals differential whey proteins of porcine milk during lactation Food Chemistry: X Porcine milk Proteomics Whey proteins Lactation |
| title | Label-free-based proteomic analysis reveals differential whey proteins of porcine milk during lactation |
| title_full | Label-free-based proteomic analysis reveals differential whey proteins of porcine milk during lactation |
| title_fullStr | Label-free-based proteomic analysis reveals differential whey proteins of porcine milk during lactation |
| title_full_unstemmed | Label-free-based proteomic analysis reveals differential whey proteins of porcine milk during lactation |
| title_short | Label-free-based proteomic analysis reveals differential whey proteins of porcine milk during lactation |
| title_sort | label free based proteomic analysis reveals differential whey proteins of porcine milk during lactation |
| topic | Porcine milk Proteomics Whey proteins Lactation |
| url | http://www.sciencedirect.com/science/article/pii/S2590157523005552 |
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