Radiotherapy Increases aMMP-8-Levels and Neutrophil/Lymphocyte Ratio Rapidly in Head and Neck Cancer Patients: A Pilot Study
Radiotherapy for head and neck carcinoma (HNC) has both curative and palliative purposes. This study investigated mouthrinse aMMP-8 levels, molecular forms of MMP-8, blood neutrophil counts and neurophil/lymphocyte ratios before and 3 weeks after HNC radiotherapy started. Thirteen HNC patients under...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2023-03-01
|
| Series: | Cancer Control |
| Online Access: | https://doi.org/10.1177/10732748231163653 |
| _version_ | 1827820386843099136 |
|---|---|
| author | Ella Brandt DDS Mutlu Keskin DDS, PhD Taina Tervahartiala DDS, PhD Mustafa Yılmaz DDS, PhD İlknur Harmankaya MD Didem Karaçetin MD Turgut İpek MD Ulvi Kahraman Gürsoy DDS, PhD Jaana Rautava DDS, PhD Shipra Gupta MDS, PhD Jaana Hagström DDS, PhD Ismo T. Räisänen DDS Timo Sorsa DDS, PhD |
| author_facet | Ella Brandt DDS Mutlu Keskin DDS, PhD Taina Tervahartiala DDS, PhD Mustafa Yılmaz DDS, PhD İlknur Harmankaya MD Didem Karaçetin MD Turgut İpek MD Ulvi Kahraman Gürsoy DDS, PhD Jaana Rautava DDS, PhD Shipra Gupta MDS, PhD Jaana Hagström DDS, PhD Ismo T. Räisänen DDS Timo Sorsa DDS, PhD |
| author_sort | Ella Brandt DDS |
| collection | DOAJ |
| description | Radiotherapy for head and neck carcinoma (HNC) has both curative and palliative purposes. This study investigated mouthrinse aMMP-8 levels, molecular forms of MMP-8, blood neutrophil counts and neurophil/lymphocyte ratios before and 3 weeks after HNC radiotherapy started. Thirteen HNC patients undergoing radiotherapy were included. Mouthrinse samples (before and 3 weeks after HNC radiotherapy had started) were assayed quantitatively by aMMP-8 point-of-care-kit (PerioSafe®/ORALyzer®) and by western immunoblot. Total neutrophil counts and neutrophil/lymphocyte ratios were evaluated in the hemogram results. Three weeks after HNC radiotherapy started, significant increases in aMMP-8 levels and neutrophil/lymphocyte ratios were observed. No significant difference was found in total neutrophil counts. Elevations of the activated and fragmented MMP-8 levels after HNC radiotherapy application were observed on western immunoblot analysis. The increase in the aMMP-8 levels and neutrophil/lymphocyte ratios indicate inflammation both locally and systemically suggesting increased risk for periodontitis due to the HNC radiotherapy. |
| first_indexed | 2024-03-12T01:27:06Z |
| format | Article |
| id | doaj.art-4001f2de7df8444b9449d8a1529cbec6 |
| institution | Directory Open Access Journal |
| issn | 1526-2359 |
| language | English |
| last_indexed | 2024-03-12T01:27:06Z |
| publishDate | 2023-03-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cancer Control |
| spelling | doaj.art-4001f2de7df8444b9449d8a1529cbec62023-09-12T13:33:55ZengSAGE PublishingCancer Control1526-23592023-03-013010.1177/10732748231163653Radiotherapy Increases aMMP-8-Levels and Neutrophil/Lymphocyte Ratio Rapidly in Head and Neck Cancer Patients: A Pilot StudyElla Brandt DDSMutlu Keskin DDS, PhDTaina Tervahartiala DDS, PhDMustafa Yılmaz DDS, PhDİlknur Harmankaya MDDidem Karaçetin MDTurgut İpek MDUlvi Kahraman Gürsoy DDS, PhDJaana Rautava DDS, PhDShipra Gupta MDS, PhDJaana Hagström DDS, PhDIsmo T. Räisänen DDSTimo Sorsa DDS, PhDRadiotherapy for head and neck carcinoma (HNC) has both curative and palliative purposes. This study investigated mouthrinse aMMP-8 levels, molecular forms of MMP-8, blood neutrophil counts and neurophil/lymphocyte ratios before and 3 weeks after HNC radiotherapy started. Thirteen HNC patients undergoing radiotherapy were included. Mouthrinse samples (before and 3 weeks after HNC radiotherapy had started) were assayed quantitatively by aMMP-8 point-of-care-kit (PerioSafe®/ORALyzer®) and by western immunoblot. Total neutrophil counts and neutrophil/lymphocyte ratios were evaluated in the hemogram results. Three weeks after HNC radiotherapy started, significant increases in aMMP-8 levels and neutrophil/lymphocyte ratios were observed. No significant difference was found in total neutrophil counts. Elevations of the activated and fragmented MMP-8 levels after HNC radiotherapy application were observed on western immunoblot analysis. The increase in the aMMP-8 levels and neutrophil/lymphocyte ratios indicate inflammation both locally and systemically suggesting increased risk for periodontitis due to the HNC radiotherapy.https://doi.org/10.1177/10732748231163653 |
| spellingShingle | Ella Brandt DDS Mutlu Keskin DDS, PhD Taina Tervahartiala DDS, PhD Mustafa Yılmaz DDS, PhD İlknur Harmankaya MD Didem Karaçetin MD Turgut İpek MD Ulvi Kahraman Gürsoy DDS, PhD Jaana Rautava DDS, PhD Shipra Gupta MDS, PhD Jaana Hagström DDS, PhD Ismo T. Räisänen DDS Timo Sorsa DDS, PhD Radiotherapy Increases aMMP-8-Levels and Neutrophil/Lymphocyte Ratio Rapidly in Head and Neck Cancer Patients: A Pilot Study Cancer Control |
| title | Radiotherapy Increases aMMP-8-Levels and Neutrophil/Lymphocyte Ratio Rapidly in Head and Neck Cancer Patients: A Pilot Study |
| title_full | Radiotherapy Increases aMMP-8-Levels and Neutrophil/Lymphocyte Ratio Rapidly in Head and Neck Cancer Patients: A Pilot Study |
| title_fullStr | Radiotherapy Increases aMMP-8-Levels and Neutrophil/Lymphocyte Ratio Rapidly in Head and Neck Cancer Patients: A Pilot Study |
| title_full_unstemmed | Radiotherapy Increases aMMP-8-Levels and Neutrophil/Lymphocyte Ratio Rapidly in Head and Neck Cancer Patients: A Pilot Study |
| title_short | Radiotherapy Increases aMMP-8-Levels and Neutrophil/Lymphocyte Ratio Rapidly in Head and Neck Cancer Patients: A Pilot Study |
| title_sort | radiotherapy increases ammp 8 levels and neutrophil lymphocyte ratio rapidly in head and neck cancer patients a pilot study |
| url | https://doi.org/10.1177/10732748231163653 |
| work_keys_str_mv | AT ellabrandtdds radiotherapyincreasesammp8levelsandneutrophillymphocyteratiorapidlyinheadandneckcancerpatientsapilotstudy AT mutlukeskinddsphd radiotherapyincreasesammp8levelsandneutrophillymphocyteratiorapidlyinheadandneckcancerpatientsapilotstudy AT tainatervahartialaddsphd radiotherapyincreasesammp8levelsandneutrophillymphocyteratiorapidlyinheadandneckcancerpatientsapilotstudy AT mustafayılmazddsphd radiotherapyincreasesammp8levelsandneutrophillymphocyteratiorapidlyinheadandneckcancerpatientsapilotstudy AT ilknurharmankayamd radiotherapyincreasesammp8levelsandneutrophillymphocyteratiorapidlyinheadandneckcancerpatientsapilotstudy AT didemkaracetinmd radiotherapyincreasesammp8levelsandneutrophillymphocyteratiorapidlyinheadandneckcancerpatientsapilotstudy AT turgutipekmd radiotherapyincreasesammp8levelsandneutrophillymphocyteratiorapidlyinheadandneckcancerpatientsapilotstudy AT ulvikahramangursoyddsphd radiotherapyincreasesammp8levelsandneutrophillymphocyteratiorapidlyinheadandneckcancerpatientsapilotstudy AT jaanarautavaddsphd radiotherapyincreasesammp8levelsandneutrophillymphocyteratiorapidlyinheadandneckcancerpatientsapilotstudy AT shipraguptamdsphd radiotherapyincreasesammp8levelsandneutrophillymphocyteratiorapidlyinheadandneckcancerpatientsapilotstudy AT jaanahagstromddsphd radiotherapyincreasesammp8levelsandneutrophillymphocyteratiorapidlyinheadandneckcancerpatientsapilotstudy AT ismotraisanendds radiotherapyincreasesammp8levelsandneutrophillymphocyteratiorapidlyinheadandneckcancerpatientsapilotstudy AT timosorsaddsphd radiotherapyincreasesammp8levelsandneutrophillymphocyteratiorapidlyinheadandneckcancerpatientsapilotstudy |