Lipid Profiles of Human Serum Fractions Enhanced with CD9 Antibody-Immobilized Magnetic Beads
Blood samples are minimally invasive and can be collected repeatedly, but they are far from the site of disease and the target molecules are diluted by the large amount of blood. Therefore, we performed lipidomics using immunoprecipitation as a method to enrich specific fractions of serum. In this s...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/12/3/230 |
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| author | Suzumi M. Tokuoka Yoshihiro Kita Masaya Sato Takao Shimizu Yutaka Yatomi Yoshiya Oda |
| author_facet | Suzumi M. Tokuoka Yoshihiro Kita Masaya Sato Takao Shimizu Yutaka Yatomi Yoshiya Oda |
| author_sort | Suzumi M. Tokuoka |
| collection | DOAJ |
| description | Blood samples are minimally invasive and can be collected repeatedly, but they are far from the site of disease and the target molecules are diluted by the large amount of blood. Therefore, we performed lipidomics using immunoprecipitation as a method to enrich specific fractions of serum. In this study, a CD9 antibody was immobilized on magnetic beads to enrich CD9-containing components in the serum for lipidomics. The percentages of phospholipids recovered from serum by methanol and isopropanol extractions were not significantly different, but triglycerides were barely recovered from serum by methanol extraction, requiring the use of isopropanol. However, once the serum was enriched with CD9 magnetic beads, triglycerides, and phospholipids were recovered at similar levels in both methanol and isopropanol extractions. Therefore, it is possible that the triglyceride fraction of the whole serum and the triglyceride fraction were enriched in CD9 magnetic beads differ in localization and properties. In addition, the variation per disease was small in general serum lipidomics; however, the difference per disease appeared larger when CD9 magnetic bead enrichment was employed. |
| first_indexed | 2024-03-09T13:21:12Z |
| format | Article |
| id | doaj.art-40039c8e3fbd4554a3fd590a8376c261 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T13:21:12Z |
| publishDate | 2022-03-01 |
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| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-40039c8e3fbd4554a3fd590a8376c2612023-11-30T21:29:41ZengMDPI AGMetabolites2218-19892022-03-0112323010.3390/metabo12030230Lipid Profiles of Human Serum Fractions Enhanced with CD9 Antibody-Immobilized Magnetic BeadsSuzumi M. Tokuoka0Yoshihiro Kita1Masaya Sato2Takao Shimizu3Yutaka Yatomi4Yoshiya Oda5Department of Lipidomics, Graduate School of Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8654, JapanDepartment of Lipidomics, Graduate School of Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8654, JapanDepartment of Clinical Laboratory Medicine, Graduate School of Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8654, JapanDepartment of Lipidomics, Graduate School of Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8654, JapanDepartment of Clinical Laboratory Medicine, Graduate School of Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8654, JapanDepartment of Lipidomics, Graduate School of Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8654, JapanBlood samples are minimally invasive and can be collected repeatedly, but they are far from the site of disease and the target molecules are diluted by the large amount of blood. Therefore, we performed lipidomics using immunoprecipitation as a method to enrich specific fractions of serum. In this study, a CD9 antibody was immobilized on magnetic beads to enrich CD9-containing components in the serum for lipidomics. The percentages of phospholipids recovered from serum by methanol and isopropanol extractions were not significantly different, but triglycerides were barely recovered from serum by methanol extraction, requiring the use of isopropanol. However, once the serum was enriched with CD9 magnetic beads, triglycerides, and phospholipids were recovered at similar levels in both methanol and isopropanol extractions. Therefore, it is possible that the triglyceride fraction of the whole serum and the triglyceride fraction were enriched in CD9 magnetic beads differ in localization and properties. In addition, the variation per disease was small in general serum lipidomics; however, the difference per disease appeared larger when CD9 magnetic bead enrichment was employed.https://www.mdpi.com/2218-1989/12/3/230immunoprecipitationCD9lipidomicshuman serum |
| spellingShingle | Suzumi M. Tokuoka Yoshihiro Kita Masaya Sato Takao Shimizu Yutaka Yatomi Yoshiya Oda Lipid Profiles of Human Serum Fractions Enhanced with CD9 Antibody-Immobilized Magnetic Beads Metabolites immunoprecipitation CD9 lipidomics human serum |
| title | Lipid Profiles of Human Serum Fractions Enhanced with CD9 Antibody-Immobilized Magnetic Beads |
| title_full | Lipid Profiles of Human Serum Fractions Enhanced with CD9 Antibody-Immobilized Magnetic Beads |
| title_fullStr | Lipid Profiles of Human Serum Fractions Enhanced with CD9 Antibody-Immobilized Magnetic Beads |
| title_full_unstemmed | Lipid Profiles of Human Serum Fractions Enhanced with CD9 Antibody-Immobilized Magnetic Beads |
| title_short | Lipid Profiles of Human Serum Fractions Enhanced with CD9 Antibody-Immobilized Magnetic Beads |
| title_sort | lipid profiles of human serum fractions enhanced with cd9 antibody immobilized magnetic beads |
| topic | immunoprecipitation CD9 lipidomics human serum |
| url | https://www.mdpi.com/2218-1989/12/3/230 |
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