HIV-1 Tat interacts with LIS1 protein
<p>Abstract</p> <p>Background</p> <p>HIV-1 Tat activates transcription of HIV-1 viral genes by inducing phosphorylation of the C-terminal domain (CTD) of RNA polymerase II (RNAPII). Tat can also disturb cellular metabolism by inhibiting proliferation of antigen-specific...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2005-02-01
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| Series: | Retrovirology |
| Online Access: | http://www.retrovirology.com/content/2/1/6 |
| _version_ | 1818002163496910848 |
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| author | Turner Willie Lane William S Voloshin Yaroslav Sapir Tamar Ammosova Tatyana Epie Nicolas Reiner Orly Nekhai Sergei |
| author_facet | Turner Willie Lane William S Voloshin Yaroslav Sapir Tamar Ammosova Tatyana Epie Nicolas Reiner Orly Nekhai Sergei |
| author_sort | Turner Willie |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>HIV-1 Tat activates transcription of HIV-1 viral genes by inducing phosphorylation of the C-terminal domain (CTD) of RNA polymerase II (RNAPII). Tat can also disturb cellular metabolism by inhibiting proliferation of antigen-specific T lymphocytes and by inducing cellular apoptosis. Tat-induced apoptosis of T-cells is attributed, in part, to the distortion of microtubules polymerization. LIS1 is a microtubule-associated protein that facilitates microtubule polymerization.</p> <p>Results</p> <p>We identified here LIS1 as a Tat-interacting protein during extensive biochemical fractionation of T-cell extracts. We found several proteins to co-purify with a Tat-associated RNAPII CTD kinase activity including LIS1, CDK7, cyclin H, and MAT1. Tat interacted with LIS1 but not with CDK7, cyclin H or MAT1 <it>in vitro</it>. LIS1 also co-immunoprecipitated with Tat expressed in HeLa cells. Further, LIS1 interacted with Tat in a yeast two-hybrid system.</p> <p>Conclusion</p> <p>Our results indicate that Tat interacts with LIS1 <it>in vitro </it>and <it>in vivo </it>and that this interaction might contribute to the effect of Tat on microtubule formation.</p> |
| first_indexed | 2024-04-14T03:42:09Z |
| format | Article |
| id | doaj.art-400693229c3047b086b90fcd48f69499 |
| institution | Directory Open Access Journal |
| issn | 1742-4690 |
| language | English |
| last_indexed | 2024-04-14T03:42:09Z |
| publishDate | 2005-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Retrovirology |
| spelling | doaj.art-400693229c3047b086b90fcd48f694992022-12-22T02:14:27ZengBMCRetrovirology1742-46902005-02-0121610.1186/1742-4690-2-6HIV-1 Tat interacts with LIS1 proteinTurner WillieLane William SVoloshin YaroslavSapir TamarAmmosova TatyanaEpie NicolasReiner OrlyNekhai Sergei<p>Abstract</p> <p>Background</p> <p>HIV-1 Tat activates transcription of HIV-1 viral genes by inducing phosphorylation of the C-terminal domain (CTD) of RNA polymerase II (RNAPII). Tat can also disturb cellular metabolism by inhibiting proliferation of antigen-specific T lymphocytes and by inducing cellular apoptosis. Tat-induced apoptosis of T-cells is attributed, in part, to the distortion of microtubules polymerization. LIS1 is a microtubule-associated protein that facilitates microtubule polymerization.</p> <p>Results</p> <p>We identified here LIS1 as a Tat-interacting protein during extensive biochemical fractionation of T-cell extracts. We found several proteins to co-purify with a Tat-associated RNAPII CTD kinase activity including LIS1, CDK7, cyclin H, and MAT1. Tat interacted with LIS1 but not with CDK7, cyclin H or MAT1 <it>in vitro</it>. LIS1 also co-immunoprecipitated with Tat expressed in HeLa cells. Further, LIS1 interacted with Tat in a yeast two-hybrid system.</p> <p>Conclusion</p> <p>Our results indicate that Tat interacts with LIS1 <it>in vitro </it>and <it>in vivo </it>and that this interaction might contribute to the effect of Tat on microtubule formation.</p>http://www.retrovirology.com/content/2/1/6 |
| spellingShingle | Turner Willie Lane William S Voloshin Yaroslav Sapir Tamar Ammosova Tatyana Epie Nicolas Reiner Orly Nekhai Sergei HIV-1 Tat interacts with LIS1 protein Retrovirology |
| title | HIV-1 Tat interacts with LIS1 protein |
| title_full | HIV-1 Tat interacts with LIS1 protein |
| title_fullStr | HIV-1 Tat interacts with LIS1 protein |
| title_full_unstemmed | HIV-1 Tat interacts with LIS1 protein |
| title_short | HIV-1 Tat interacts with LIS1 protein |
| title_sort | hiv 1 tat interacts with lis1 protein |
| url | http://www.retrovirology.com/content/2/1/6 |
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