Inhibition of IRE1 modifies hypoxic regulation of G6PD, GPI, TKT, TALDO1, PGLS and RPIA genes expression in U87 glioma cells
We have studied the effect of hypoxia on the expression level of mRNA of the basic enzymes of pentose-phosphate cycle (G6PD, TKT, TALDO1, PGLS and RPIA) and glucose-6-phosphate isomerase (GPI) in U87 glioma cells in relation to inhibition of IRE1 (inositol requiring enzyme 1). It was shown that hypo...
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2017-02-01
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Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2017/01/Minchenko_1_17.pdf |
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author | O. H. Minchenko I. A. Garmash D. O. Minchenko A. Y. Kuznetsova O. O. Ratushna |
author_facet | O. H. Minchenko I. A. Garmash D. O. Minchenko A. Y. Kuznetsova O. O. Ratushna |
author_sort | O. H. Minchenko |
collection | DOAJ |
description | We have studied the effect of hypoxia on the expression level of mRNA of the basic enzymes of pentose-phosphate cycle (G6PD, TKT, TALDO1, PGLS and RPIA) and glucose-6-phosphate isomerase (GPI) in U87 glioma cells in relation to inhibition of IRE1 (inositol requiring enzyme 1). It was shown that hypoxia leads to up-regulation of the expression of GPI and PGLS genes and to down-regulation of TALDO1 and RPIA genes in control glioma cells. Changes for GPI gene were more significant than for other genes. At the same time, inhibition of IRE1 modified the effect of hypoxia on the expression of all studied genes. In particular, it increased sensitivity to hypoxia of G6PD and TKT genes expression and suppressed the effect of hypoxia on the expression of GPI and RPIA genes. Additionally, inhibition of IRE1 eliminated hypoxic regulation of PGLS gene and did not change significantly effect of hypoxia on the expression of TALDO1 gene in glioma cells. Present study demonstrated that hypoxia, which often contributes to tumor growth, affects the expression of most studied genes and inhibition of IRE1 modified the hypoxic regulation of pentose-phosphate cycle gene expressions in a gene specific manner and thus possibly contributes to slower glioma growth, but several aspects of this regulation warrant further investigation. |
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issn | 2409-4943 2413-5003 |
language | English |
last_indexed | 2024-03-11T13:49:59Z |
publishDate | 2017-02-01 |
publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
record_format | Article |
series | The Ukrainian Biochemical Journal |
spelling | doaj.art-4006cdfdcc244cbc85c50b14427b151c2023-11-02T09:10:47ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032017-02-01891384910.15407/ubj89.01.038Inhibition of IRE1 modifies hypoxic regulation of G6PD, GPI, TKT, TALDO1, PGLS and RPIA genes expression in U87 glioma cellsO. H. Minchenko0I. A. Garmash1D. O. Minchenko2A. Y. Kuznetsova3O. O. Ratushna41Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv1Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, KyivPalladin Institute of Biochemistry, National Academy of Sciences of Ukraine, KyivPalladin Institute of Biochemistry, National Academy of Sciences of Ukraine, KyivPalladin Institute of Biochemistry, National Academy of Sciences of Ukraine, KyivWe have studied the effect of hypoxia on the expression level of mRNA of the basic enzymes of pentose-phosphate cycle (G6PD, TKT, TALDO1, PGLS and RPIA) and glucose-6-phosphate isomerase (GPI) in U87 glioma cells in relation to inhibition of IRE1 (inositol requiring enzyme 1). It was shown that hypoxia leads to up-regulation of the expression of GPI and PGLS genes and to down-regulation of TALDO1 and RPIA genes in control glioma cells. Changes for GPI gene were more significant than for other genes. At the same time, inhibition of IRE1 modified the effect of hypoxia on the expression of all studied genes. In particular, it increased sensitivity to hypoxia of G6PD and TKT genes expression and suppressed the effect of hypoxia on the expression of GPI and RPIA genes. Additionally, inhibition of IRE1 eliminated hypoxic regulation of PGLS gene and did not change significantly effect of hypoxia on the expression of TALDO1 gene in glioma cells. Present study demonstrated that hypoxia, which often contributes to tumor growth, affects the expression of most studied genes and inhibition of IRE1 modified the hypoxic regulation of pentose-phosphate cycle gene expressions in a gene specific manner and thus possibly contributes to slower glioma growth, but several aspects of this regulation warrant further investigation.http://ukrbiochemjournal.org/wp-content/uploads/2017/01/Minchenko_1_17.pdfG6PDIRE1 inhibitionGPIhypoxia |
spellingShingle | O. H. Minchenko I. A. Garmash D. O. Minchenko A. Y. Kuznetsova O. O. Ratushna Inhibition of IRE1 modifies hypoxic regulation of G6PD, GPI, TKT, TALDO1, PGLS and RPIA genes expression in U87 glioma cells The Ukrainian Biochemical Journal G6PD IRE1 inhibition GPI hypoxia |
title | Inhibition of IRE1 modifies hypoxic regulation of G6PD, GPI, TKT, TALDO1, PGLS and RPIA genes expression in U87 glioma cells |
title_full | Inhibition of IRE1 modifies hypoxic regulation of G6PD, GPI, TKT, TALDO1, PGLS and RPIA genes expression in U87 glioma cells |
title_fullStr | Inhibition of IRE1 modifies hypoxic regulation of G6PD, GPI, TKT, TALDO1, PGLS and RPIA genes expression in U87 glioma cells |
title_full_unstemmed | Inhibition of IRE1 modifies hypoxic regulation of G6PD, GPI, TKT, TALDO1, PGLS and RPIA genes expression in U87 glioma cells |
title_short | Inhibition of IRE1 modifies hypoxic regulation of G6PD, GPI, TKT, TALDO1, PGLS and RPIA genes expression in U87 glioma cells |
title_sort | inhibition of ire1 modifies hypoxic regulation of g6pd gpi tkt taldo1 pgls and rpia genes expression in u87 glioma cells |
topic | G6PD IRE1 inhibition GPI hypoxia |
url | http://ukrbiochemjournal.org/wp-content/uploads/2017/01/Minchenko_1_17.pdf |
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