Transcriptional responses of Rosa rugosa to salt stress and salt shock
ABSTRACT Rugosa rugosa has high tolerance to various stresses; however, the molecular mechanisms of this behavior under adverse conditions are unclear. The objective of this study is to investigate expression patterns of stress-related genes in response to salinity stress. Changes in transcript leve...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade Federal de Lavras
2020-11-01
|
| Series: | Ciência e Agrotecnologia |
| Subjects: | |
| Online Access: | http://www.scielo.br/pdf/cagro/v44/1981-1829-cagro-44-e008220.pdf |
| _version_ | 1818753100250349568 |
|---|---|
| author | Michele Valquíria dos Reis Laura Vaughn Rouhana Patrícia Duarte de Oliveira Paiva Diogo Pedrosa Correia da Silva Renato Paiva Schuyler Korban |
| author_facet | Michele Valquíria dos Reis Laura Vaughn Rouhana Patrícia Duarte de Oliveira Paiva Diogo Pedrosa Correia da Silva Renato Paiva Schuyler Korban |
| author_sort | Michele Valquíria dos Reis |
| collection | DOAJ |
| description | ABSTRACT Rugosa rugosa has high tolerance to various stresses; however, the molecular mechanisms of this behavior under adverse conditions are unclear. The objective of this study is to investigate expression patterns of stress-related genes in response to salinity stress. Changes in transcript levels of R. rugose, grown under different salt stress conditions (0, 25, 50, and 100 mM NaCl) over a long exposure period (30 days), have been investigated. In addition, the effects of salt shock stress on seedlings exposed to a high level (200 mM) of NaCl for a relatively short duration (3 h) have also been investigated. Expression levels of selected differentially expressed genes have been determined using relative reverse transcription polymerase chain reaction (RT-PCR). It has been observed that seedlings exposed to salt stress for a long duration exhibited no signs of stress in both leaves and roots. In addition, expression of NHX1 in R. rugosa increased in the presence of NaCl. Furthermore, transcripts of EXP4, GPP, NHX1, NAC, and DREB genes also increased under high levels of NaCl. In contrast, expression levels of MYB and TIR decreased during this salt shock treatment. Of particular interest is the increase in levels of transcripts of NHX1 in leaves of seedlings grown under both salt stress and salt shock conditions, thus suggesting that this gene plays an important role in salt stress tolerance in R. rugosa. These findings will support efforts in enhancing salt tolerance in roses, and perhaps in other members of the Rosaceae family. |
| first_indexed | 2024-12-18T05:01:59Z |
| format | Article |
| id | doaj.art-40099e9ae11e444e9f448afb2957fad6 |
| institution | Directory Open Access Journal |
| issn | 1981-1829 |
| language | English |
| last_indexed | 2024-12-18T05:01:59Z |
| publishDate | 2020-11-01 |
| publisher | Universidade Federal de Lavras |
| record_format | Article |
| series | Ciência e Agrotecnologia |
| spelling | doaj.art-40099e9ae11e444e9f448afb2957fad62022-12-21T21:20:07ZengUniversidade Federal de LavrasCiência e Agrotecnologia1981-18292020-11-014410.1590/1413-7054202044008220Transcriptional responses of Rosa rugosa to salt stress and salt shockMichele Valquíria dos Reishttps://orcid.org/0000-0003-0379-2384Laura Vaughn Rouhanahttps://orcid.org/0000-0003-0152-1791Patrícia Duarte de Oliveira Paivahttps://orcid.org/0000-0001-7997-8420Diogo Pedrosa Correia da Silvahttps://orcid.org/0000-0002-7787-7500Renato Paivahttps://orcid.org/0000-0001-5107-0285Schuyler Korbanhttps://orcid.org/0000-0003-1544-5160ABSTRACT Rugosa rugosa has high tolerance to various stresses; however, the molecular mechanisms of this behavior under adverse conditions are unclear. The objective of this study is to investigate expression patterns of stress-related genes in response to salinity stress. Changes in transcript levels of R. rugose, grown under different salt stress conditions (0, 25, 50, and 100 mM NaCl) over a long exposure period (30 days), have been investigated. In addition, the effects of salt shock stress on seedlings exposed to a high level (200 mM) of NaCl for a relatively short duration (3 h) have also been investigated. Expression levels of selected differentially expressed genes have been determined using relative reverse transcription polymerase chain reaction (RT-PCR). It has been observed that seedlings exposed to salt stress for a long duration exhibited no signs of stress in both leaves and roots. In addition, expression of NHX1 in R. rugosa increased in the presence of NaCl. Furthermore, transcripts of EXP4, GPP, NHX1, NAC, and DREB genes also increased under high levels of NaCl. In contrast, expression levels of MYB and TIR decreased during this salt shock treatment. Of particular interest is the increase in levels of transcripts of NHX1 in leaves of seedlings grown under both salt stress and salt shock conditions, thus suggesting that this gene plays an important role in salt stress tolerance in R. rugosa. These findings will support efforts in enhancing salt tolerance in roses, and perhaps in other members of the Rosaceae family.http://www.scielo.br/pdf/cagro/v44/1981-1829-cagro-44-e008220.pdfRosaceaesalinitysalt toleranceion homeostasis |
| spellingShingle | Michele Valquíria dos Reis Laura Vaughn Rouhana Patrícia Duarte de Oliveira Paiva Diogo Pedrosa Correia da Silva Renato Paiva Schuyler Korban Transcriptional responses of Rosa rugosa to salt stress and salt shock Ciência e Agrotecnologia Rosaceae salinity salt tolerance ion homeostasis |
| title | Transcriptional responses of Rosa rugosa to salt stress and salt shock |
| title_full | Transcriptional responses of Rosa rugosa to salt stress and salt shock |
| title_fullStr | Transcriptional responses of Rosa rugosa to salt stress and salt shock |
| title_full_unstemmed | Transcriptional responses of Rosa rugosa to salt stress and salt shock |
| title_short | Transcriptional responses of Rosa rugosa to salt stress and salt shock |
| title_sort | transcriptional responses of rosa rugosa to salt stress and salt shock |
| topic | Rosaceae salinity salt tolerance ion homeostasis |
| url | http://www.scielo.br/pdf/cagro/v44/1981-1829-cagro-44-e008220.pdf |
| work_keys_str_mv | AT michelevalquiriadosreis transcriptionalresponsesofrosarugosatosaltstressandsaltshock AT lauravaughnrouhana transcriptionalresponsesofrosarugosatosaltstressandsaltshock AT patriciaduartedeoliveirapaiva transcriptionalresponsesofrosarugosatosaltstressandsaltshock AT diogopedrosacorreiadasilva transcriptionalresponsesofrosarugosatosaltstressandsaltshock AT renatopaiva transcriptionalresponsesofrosarugosatosaltstressandsaltshock AT schuylerkorban transcriptionalresponsesofrosarugosatosaltstressandsaltshock |