Generation and characterization of three fibroblast-derived Rhesus Macaque induced pluripotent stem cell lines
Cross-species comparisons using pluripotent stem cells from primates are crucial to better understand human biology, disease, and evolution. An important primate model is the Rhesus macaque (Macaca mulatta), and we reprogrammed skin fibroblasts from a male individual to generate three induced plurip...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-02-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506123002635 |
| _version_ | 1797356326279446528 |
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| author | Jessica Jocher Fiona C. Edenhofer Philipp Janssen Stefan Müller Dana C. Lopez-Parra Johanna Geuder Wolfgang Enard |
| author_facet | Jessica Jocher Fiona C. Edenhofer Philipp Janssen Stefan Müller Dana C. Lopez-Parra Johanna Geuder Wolfgang Enard |
| author_sort | Jessica Jocher |
| collection | DOAJ |
| description | Cross-species comparisons using pluripotent stem cells from primates are crucial to better understand human biology, disease, and evolution. An important primate model is the Rhesus macaque (Macaca mulatta), and we reprogrammed skin fibroblasts from a male individual to generate three induced pluripotent stem cell (iPSC) lines. These cells exhibit the typical ESC-like colony morphology, express common pluripotency markers, and can differentiate into cells of the three germ layers. All generated iPSC lines can be cultured under feeder-free conditions in commercially available medium and are therefore valuable resources for cross-species comparisons. |
| first_indexed | 2024-03-08T14:25:59Z |
| format | Article |
| id | doaj.art-4009b62e3e9b46d5aba6dcd925c30ecb |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-03-08T14:25:59Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-4009b62e3e9b46d5aba6dcd925c30ecb2024-01-13T04:43:49ZengElsevierStem Cell Research1873-50612024-02-0174103277Generation and characterization of three fibroblast-derived Rhesus Macaque induced pluripotent stem cell linesJessica Jocher0Fiona C. Edenhofer1Philipp Janssen2Stefan Müller3Dana C. Lopez-Parra4Johanna Geuder5Wolfgang Enard6Anthropology & Human Genomics, Faculty of Biology, Ludwig-Maximilians-Universität München, Großhaderner Straße 2, 82152 Martinsried, GermanyAnthropology & Human Genomics, Faculty of Biology, Ludwig-Maximilians-Universität München, Großhaderner Straße 2, 82152 Martinsried, GermanyAnthropology & Human Genomics, Faculty of Biology, Ludwig-Maximilians-Universität München, Großhaderner Straße 2, 82152 Martinsried, GermanyInstitute of Human Genomics, Munich University Hospital, Ludwig-Maximilians-Universität München, 80336 Munich, GermanyAnthropology & Human Genomics, Faculty of Biology, Ludwig-Maximilians-Universität München, Großhaderner Straße 2, 82152 Martinsried, GermanyAnthropology & Human Genomics, Faculty of Biology, Ludwig-Maximilians-Universität München, Großhaderner Straße 2, 82152 Martinsried, GermanyAnthropology & Human Genomics, Faculty of Biology, Ludwig-Maximilians-Universität München, Großhaderner Straße 2, 82152 Martinsried, Germany; Corresponding author.Cross-species comparisons using pluripotent stem cells from primates are crucial to better understand human biology, disease, and evolution. An important primate model is the Rhesus macaque (Macaca mulatta), and we reprogrammed skin fibroblasts from a male individual to generate three induced pluripotent stem cell (iPSC) lines. These cells exhibit the typical ESC-like colony morphology, express common pluripotency markers, and can differentiate into cells of the three germ layers. All generated iPSC lines can be cultured under feeder-free conditions in commercially available medium and are therefore valuable resources for cross-species comparisons.http://www.sciencedirect.com/science/article/pii/S1873506123002635 |
| spellingShingle | Jessica Jocher Fiona C. Edenhofer Philipp Janssen Stefan Müller Dana C. Lopez-Parra Johanna Geuder Wolfgang Enard Generation and characterization of three fibroblast-derived Rhesus Macaque induced pluripotent stem cell lines Stem Cell Research |
| title | Generation and characterization of three fibroblast-derived Rhesus Macaque induced pluripotent stem cell lines |
| title_full | Generation and characterization of three fibroblast-derived Rhesus Macaque induced pluripotent stem cell lines |
| title_fullStr | Generation and characterization of three fibroblast-derived Rhesus Macaque induced pluripotent stem cell lines |
| title_full_unstemmed | Generation and characterization of three fibroblast-derived Rhesus Macaque induced pluripotent stem cell lines |
| title_short | Generation and characterization of three fibroblast-derived Rhesus Macaque induced pluripotent stem cell lines |
| title_sort | generation and characterization of three fibroblast derived rhesus macaque induced pluripotent stem cell lines |
| url | http://www.sciencedirect.com/science/article/pii/S1873506123002635 |
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