Identification and assessment of variable single-copy orthologous (SCO) nuclear loci for low-level phylogenomics: a case study in the genus Rosa (Rosaceae)
Abstract Background With an ever-growing number of published genomes, many low levels of the Tree of Life now contain several species with enough molecular data to perform shallow-scale phylogenomic studies. Moving away from using just a few universal phylogenetic markers, we can now target thousand...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-07-01
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| Series: | BMC Evolutionary Biology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12862-019-1479-z |
| _version_ | 1818583513817939968 |
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| author | Kevin Debray Jordan Marie-Magdelaine Tom Ruttink Jérémy Clotault Fabrice Foucher Valéry Malécot |
| author_facet | Kevin Debray Jordan Marie-Magdelaine Tom Ruttink Jérémy Clotault Fabrice Foucher Valéry Malécot |
| author_sort | Kevin Debray |
| collection | DOAJ |
| description | Abstract Background With an ever-growing number of published genomes, many low levels of the Tree of Life now contain several species with enough molecular data to perform shallow-scale phylogenomic studies. Moving away from using just a few universal phylogenetic markers, we can now target thousands of other loci to decipher taxa relationships. Making the best possible selection of informative sequences regarding the taxa studied has emerged as a new issue. Here, we developed a general procedure to mine genomic data, looking for orthologous single-copy loci capable of deciphering phylogenetic relationships below the generic rank. To develop our strategy, we chose the genus Rosa, a rapid-evolving lineage of the Rosaceae family in which several species genomes have recently been sequenced. We also compared our loci to conventional plastid markers, commonly used for phylogenetic inference in this genus. Results We generated 1856 sequence tags in putative single-copy orthologous nuclear loci. Associated in silico primer pairs can potentially amplify fragments able to resolve a wide range of speciation events within the genus Rosa. Analysis of parsimony-informative site content showed the value of non-coding genomic regions to obtain variable sequences despite the fact that they may be more difficult to target in less related species. Dozens of nuclear loci outperform the conventional plastid phylogenetic markers in terms of phylogenetic informativeness, for both recent and ancient evolutionary divergences. However, conflicting phylogenetic signals were found between nuclear gene tree topologies and the species-tree topology, shedding light on the many patterns of hybridization and/or incomplete lineage sorting that occur in the genus Rosa. Conclusions With recently published genome sequence data, we developed a set of single-copy orthologous nuclear loci to resolve species-level phylogenomics in the genus Rosa. This genome-wide scale dataset contains hundreds of highly variable loci which phylogenetic interest was assessed in terms of phylogenetic informativeness and topological conflict. Our target identification procedure can easily be reproduced to identify new highly informative loci for other taxonomic groups and ranks. |
| first_indexed | 2024-12-16T08:06:29Z |
| format | Article |
| id | doaj.art-400cd2c6b9c8487d856009fb32e9c58f |
| institution | Directory Open Access Journal |
| issn | 1471-2148 |
| language | English |
| last_indexed | 2024-12-16T08:06:29Z |
| publishDate | 2019-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Evolutionary Biology |
| spelling | doaj.art-400cd2c6b9c8487d856009fb32e9c58f2022-12-21T22:38:27ZengBMCBMC Evolutionary Biology1471-21482019-07-0119111910.1186/s12862-019-1479-zIdentification and assessment of variable single-copy orthologous (SCO) nuclear loci for low-level phylogenomics: a case study in the genus Rosa (Rosaceae)Kevin Debray0Jordan Marie-Magdelaine1Tom Ruttink2Jérémy Clotault3Fabrice Foucher4Valéry Malécot5IRHS, Agrocampus-Ouest, INRA, UNIV Angers, SFR 4207 QuaSaVIRHS, Agrocampus-Ouest, INRA, UNIV Angers, SFR 4207 QuaSaVILVO, Flanders Research Institute for Agriculture, Fisheries and Food, Plant Sciences UnitIRHS, Agrocampus-Ouest, INRA, UNIV Angers, SFR 4207 QuaSaVIRHS, Agrocampus-Ouest, INRA, UNIV Angers, SFR 4207 QuaSaVIRHS, Agrocampus-Ouest, INRA, UNIV Angers, SFR 4207 QuaSaVAbstract Background With an ever-growing number of published genomes, many low levels of the Tree of Life now contain several species with enough molecular data to perform shallow-scale phylogenomic studies. Moving away from using just a few universal phylogenetic markers, we can now target thousands of other loci to decipher taxa relationships. Making the best possible selection of informative sequences regarding the taxa studied has emerged as a new issue. Here, we developed a general procedure to mine genomic data, looking for orthologous single-copy loci capable of deciphering phylogenetic relationships below the generic rank. To develop our strategy, we chose the genus Rosa, a rapid-evolving lineage of the Rosaceae family in which several species genomes have recently been sequenced. We also compared our loci to conventional plastid markers, commonly used for phylogenetic inference in this genus. Results We generated 1856 sequence tags in putative single-copy orthologous nuclear loci. Associated in silico primer pairs can potentially amplify fragments able to resolve a wide range of speciation events within the genus Rosa. Analysis of parsimony-informative site content showed the value of non-coding genomic regions to obtain variable sequences despite the fact that they may be more difficult to target in less related species. Dozens of nuclear loci outperform the conventional plastid phylogenetic markers in terms of phylogenetic informativeness, for both recent and ancient evolutionary divergences. However, conflicting phylogenetic signals were found between nuclear gene tree topologies and the species-tree topology, shedding light on the many patterns of hybridization and/or incomplete lineage sorting that occur in the genus Rosa. Conclusions With recently published genome sequence data, we developed a set of single-copy orthologous nuclear loci to resolve species-level phylogenomics in the genus Rosa. This genome-wide scale dataset contains hundreds of highly variable loci which phylogenetic interest was assessed in terms of phylogenetic informativeness and topological conflict. Our target identification procedure can easily be reproduced to identify new highly informative loci for other taxonomic groups and ranks.http://link.springer.com/article/10.1186/s12862-019-1479-zSpecies-level phylogenomicsNuclear single-copy orthologsPhylogenetic informativenessConflicting topologies |
| spellingShingle | Kevin Debray Jordan Marie-Magdelaine Tom Ruttink Jérémy Clotault Fabrice Foucher Valéry Malécot Identification and assessment of variable single-copy orthologous (SCO) nuclear loci for low-level phylogenomics: a case study in the genus Rosa (Rosaceae) BMC Evolutionary Biology Species-level phylogenomics Nuclear single-copy orthologs Phylogenetic informativeness Conflicting topologies |
| title | Identification and assessment of variable single-copy orthologous (SCO) nuclear loci for low-level phylogenomics: a case study in the genus Rosa (Rosaceae) |
| title_full | Identification and assessment of variable single-copy orthologous (SCO) nuclear loci for low-level phylogenomics: a case study in the genus Rosa (Rosaceae) |
| title_fullStr | Identification and assessment of variable single-copy orthologous (SCO) nuclear loci for low-level phylogenomics: a case study in the genus Rosa (Rosaceae) |
| title_full_unstemmed | Identification and assessment of variable single-copy orthologous (SCO) nuclear loci for low-level phylogenomics: a case study in the genus Rosa (Rosaceae) |
| title_short | Identification and assessment of variable single-copy orthologous (SCO) nuclear loci for low-level phylogenomics: a case study in the genus Rosa (Rosaceae) |
| title_sort | identification and assessment of variable single copy orthologous sco nuclear loci for low level phylogenomics a case study in the genus rosa rosaceae |
| topic | Species-level phylogenomics Nuclear single-copy orthologs Phylogenetic informativeness Conflicting topologies |
| url | http://link.springer.com/article/10.1186/s12862-019-1479-z |
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