Generation of a NKX2.1 – p63 double transgenic knock-in reporter cell line from human induced pluripotent stem cells (MHHi006-A-4)
Tumor protein p63 (p63) encodes for a transcription factor of the p53 family and is a marker for respiratory basal cells. Based on a NKX2.1 knock-in reporter cell line from human induced pluripotent stem cells (hiPSCs) (MHHi06-A-2) we established a NKX2.1/p63 double transgenic knock-in reporter cell...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-01-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506119302892 |
| _version_ | 1818512657343315968 |
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| author | Nora Drick Anais Sahabian Praeploy Pongpamorn Sylvia Merkert Gudrun Göhring Tobias Welte Ulrich Martin Ruth Olmer |
| author_facet | Nora Drick Anais Sahabian Praeploy Pongpamorn Sylvia Merkert Gudrun Göhring Tobias Welte Ulrich Martin Ruth Olmer |
| author_sort | Nora Drick |
| collection | DOAJ |
| description | Tumor protein p63 (p63) encodes for a transcription factor of the p53 family and is a marker for respiratory basal cells. Based on a NKX2.1 knock-in reporter cell line from human induced pluripotent stem cells (hiPSCs) (MHHi06-A-2) we established a NKX2.1/p63 double transgenic knock-in reporter cell line using TALEN technology. The reporter enables the optimization and monitoring of hiPSC differentiation towards NKX2.1/p63 double positive cells as well as enrichment for single or double positive cells. |
| first_indexed | 2024-12-10T23:49:57Z |
| format | Article |
| id | doaj.art-400d3f190718443f8c8f9c7988bac840 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-10T23:49:57Z |
| publishDate | 2020-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-400d3f190718443f8c8f9c7988bac8402022-12-22T01:28:47ZengElsevierStem Cell Research1873-50612020-01-0142Generation of a NKX2.1 – p63 double transgenic knock-in reporter cell line from human induced pluripotent stem cells (MHHi006-A-4)Nora Drick0Anais Sahabian1Praeploy Pongpamorn2Sylvia Merkert3Gudrun Göhring4Tobias Welte5Ulrich Martin6Ruth Olmer7Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, 30625 Hannover, Germany; Biomedical Research in Endstage and Obstructive Lung Disease (BREATH), Member of the German Center for Lung Research (DZL), Germany; Department of Pneumology and German Center for Lung Research, Hannover Medical School, 30625 Hannover, GermanyLeibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, 30625 Hannover, Germany; REBIRTH-Cluster of Excellence, Hannover Medical School, 30625 Hannover, Germany; Biomedical Research in Endstage and Obstructive Lung Disease (BREATH), Member of the German Center for Lung Research (DZL), GermanyLeibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, 30625 Hannover, Germany; REBIRTH-Cluster of Excellence, Hannover Medical School, 30625 Hannover, Germany; Biomedical Research in Endstage and Obstructive Lung Disease (BREATH), Member of the German Center for Lung Research (DZL), GermanyLeibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, 30625 Hannover, Germany; REBIRTH-Cluster of Excellence, Hannover Medical School, 30625 Hannover, Germany; Biomedical Research in Endstage and Obstructive Lung Disease (BREATH), Member of the German Center for Lung Research (DZL), GermanyDepartment of Human Genetics, Hannover Medical School, 30625 Hannover, GermanyBiomedical Research in Endstage and Obstructive Lung Disease (BREATH), Member of the German Center for Lung Research (DZL), Germany; Department of Pneumology and German Center for Lung Research, Hannover Medical School, 30625 Hannover, GermanyLeibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, 30625 Hannover, Germany; REBIRTH-Cluster of Excellence, Hannover Medical School, 30625 Hannover, Germany; Biomedical Research in Endstage and Obstructive Lung Disease (BREATH), Member of the German Center for Lung Research (DZL), GermanyLeibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, 30625 Hannover, Germany; REBIRTH-Cluster of Excellence, Hannover Medical School, 30625 Hannover, Germany; Biomedical Research in Endstage and Obstructive Lung Disease (BREATH), Member of the German Center for Lung Research (DZL), Germany; Corresponding author.Tumor protein p63 (p63) encodes for a transcription factor of the p53 family and is a marker for respiratory basal cells. Based on a NKX2.1 knock-in reporter cell line from human induced pluripotent stem cells (hiPSCs) (MHHi06-A-2) we established a NKX2.1/p63 double transgenic knock-in reporter cell line using TALEN technology. The reporter enables the optimization and monitoring of hiPSC differentiation towards NKX2.1/p63 double positive cells as well as enrichment for single or double positive cells.http://www.sciencedirect.com/science/article/pii/S1873506119302892 |
| spellingShingle | Nora Drick Anais Sahabian Praeploy Pongpamorn Sylvia Merkert Gudrun Göhring Tobias Welte Ulrich Martin Ruth Olmer Generation of a NKX2.1 – p63 double transgenic knock-in reporter cell line from human induced pluripotent stem cells (MHHi006-A-4) Stem Cell Research |
| title | Generation of a NKX2.1 – p63 double transgenic knock-in reporter cell line from human induced pluripotent stem cells (MHHi006-A-4) |
| title_full | Generation of a NKX2.1 – p63 double transgenic knock-in reporter cell line from human induced pluripotent stem cells (MHHi006-A-4) |
| title_fullStr | Generation of a NKX2.1 – p63 double transgenic knock-in reporter cell line from human induced pluripotent stem cells (MHHi006-A-4) |
| title_full_unstemmed | Generation of a NKX2.1 – p63 double transgenic knock-in reporter cell line from human induced pluripotent stem cells (MHHi006-A-4) |
| title_short | Generation of a NKX2.1 – p63 double transgenic knock-in reporter cell line from human induced pluripotent stem cells (MHHi006-A-4) |
| title_sort | generation of a nkx2 1 p63 double transgenic knock in reporter cell line from human induced pluripotent stem cells mhhi006 a 4 |
| url | http://www.sciencedirect.com/science/article/pii/S1873506119302892 |
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