Study of Antibacterial Activities of Seeds Extract of Fenugreek (Trigonella foenum-graecum)
Background: The antibacterial activity of seeds methanol extract and hot aqueous extract of fenugreek (Trigonella foenum-graecum) showed the presence of activity against four species of bacteria and the methanol extract have higher activity than other extract. Objective: To examine the anti...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
College of medicine/ University of Diyala
2018-09-01
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| Series: | Diyala Journal of Medicine |
| Subjects: | |
| Online Access: | http://djm.uodiyala.edu.iq/index.php/djm/article/view/117 |
| Summary: | Background: The antibacterial activity of seeds methanol extract and hot aqueous extract of fenugreek (Trigonella foenum-graecum) showed the presence of activity against four species of bacteria and the methanol extract have higher activity than other extract.
Objective: To examine the antimicrobial activities of the crude methanolic extract and hot aqueous extract of seeds of Trigonella foenum-graecum.
Patients and Methods: We prepared three different concentrations for all extracts ( 75% ,50%, 25%, v/v ). The study detected antibacterial activity by using well diffusion in agar technique .The study applied on the Four species of bacteria includes :Staphylococcus aureus , Bacillus subtilus, Pseudomonas aeruginosa and Escherichia coli. The study continued from September 2016 to January 2017 in the Laboratory of Biotechnology in College of Science - University of Diyala.
Results: The study showed highest antibacterial activity of methanolic extract of seeds of Trigonella foenum –graecum that attained highest zone of inhibition (30 mm) against P. aeruginosa when using (75%) concentration,While attained lowest zone of inhibition (10 mm) against E.coli when using (25%) concentration. Whereas the hot aqueous extract of seeds of Trigonella foenum –graecum that attained highest zone of inhibition (24 mm) against P. aeruginosa when using (75%) concentration, while don't give any effect against bacterial spp. when using (25%)concentration.
Conclusion: Antibacterial activity of methanol extract of seeds of Trigonella foenum-graecum was higher than the hot aqueous extract also higher than commercial antibiotic (Amikacin).
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| ISSN: | 2219-9764 2617-8982 |