A mutation in the cytosolic O-acetylserine (thiol) lyase induces a genome-dependent early leaf death phenotype in <it>Arabidopsis</it>
<p>Abstract</p> <p>Background</p> <p>Cysteine is a component in organic compounds including glutathione that have been implicated in the adaptation of plants to stresses. <it>O</it>-acetylserine (thiol) lyase (OAS-TL) catalyses the final step of cysteine bio...
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| Format: | Article |
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BMC
2010-04-01
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| Series: | BMC Plant Biology |
| Online Access: | http://www.biomedcentral.com/1471-2229/10/80 |
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| author | Schippers Jos HM Everts Gerja E Jing Hai-Chun Shirzadian-Khorramabad Reza Hille Jacques Dijkwel Paul P |
| author_facet | Schippers Jos HM Everts Gerja E Jing Hai-Chun Shirzadian-Khorramabad Reza Hille Jacques Dijkwel Paul P |
| author_sort | Schippers Jos HM |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Cysteine is a component in organic compounds including glutathione that have been implicated in the adaptation of plants to stresses. <it>O</it>-acetylserine (thiol) lyase (OAS-TL) catalyses the final step of cysteine biosynthesis. OAS-TL enzyme isoforms are localised in the cytoplasm, the plastids and mitochondria but the contribution of individual OAS-TL isoforms to plant sulphur metabolism has not yet been fully clarified.</p> <p>Results</p> <p>The seedling lethal phenotype of the <it>Arabidopsis onset of leaf death3-1 </it>(<it>old3-1</it>) mutant is due to a point mutation in the <it>OAS-A1 </it>gene, encoding the cytosolic OAS-TL. The mutation causes a single amino acid substitution from Gly<sup>162 </sup>to Glu<sup>162</sup>, abolishing old3-1 OAS-TL activity <it>in vitro</it>. The <it>old3-1 </it>mutation segregates as a monogenic semi-dominant trait when backcrossed to its wild type accession Landsberg <it>erecta </it>(L<it>er</it>-0) and the Di-2 accession. Consistent with its semi-dominant behaviour, wild type L<it>er</it>-0 plants transformed with the mutated <it>old3-1 </it>gene, displayed the early leaf death phenotype. However, the <it>old3-1 </it>mutation segregates in an 11:4:1 (wild type: semi-dominant: mutant) ratio when backcrossed to the Colombia-0 and Wassilewskija accessions. Thus, the early leaf death phenotype depends on two semi-dominant loci. The second locus that determines the <it>old3-1 </it>early leaf death phenotype is referred to as <it>odd-ler </it>(for <it>old3 </it>determinant in the L<it>er </it>accession) and is located on chromosome 3. The early leaf death phenotype is temperature dependent and is associated with increased expression of defence-response and oxidative-stress marker genes. Independent of the presence of the <it>odd-ler </it>gene, <it>OAS-A1 </it>is involved in maintaining sulphur and thiol levels and is required for resistance against cadmium stress.</p> <p>Conclusions</p> <p>The cytosolic OAS-TL is involved in maintaining organic sulphur levels. The <it>old3-1 </it>mutation causes genome-dependent and independent phenotypes and uncovers a novel function for the mutated OAS-TL in cell death regulation.</p> |
| first_indexed | 2024-12-11T14:27:59Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-11T14:27:59Z |
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| spelling | doaj.art-400e0235e95147b4aaad15c71a60cf4e2022-12-22T01:02:35ZengBMCBMC Plant Biology1471-22292010-04-011018010.1186/1471-2229-10-80A mutation in the cytosolic O-acetylserine (thiol) lyase induces a genome-dependent early leaf death phenotype in <it>Arabidopsis</it>Schippers Jos HMEverts Gerja EJing Hai-ChunShirzadian-Khorramabad RezaHille JacquesDijkwel Paul P<p>Abstract</p> <p>Background</p> <p>Cysteine is a component in organic compounds including glutathione that have been implicated in the adaptation of plants to stresses. <it>O</it>-acetylserine (thiol) lyase (OAS-TL) catalyses the final step of cysteine biosynthesis. OAS-TL enzyme isoforms are localised in the cytoplasm, the plastids and mitochondria but the contribution of individual OAS-TL isoforms to plant sulphur metabolism has not yet been fully clarified.</p> <p>Results</p> <p>The seedling lethal phenotype of the <it>Arabidopsis onset of leaf death3-1 </it>(<it>old3-1</it>) mutant is due to a point mutation in the <it>OAS-A1 </it>gene, encoding the cytosolic OAS-TL. The mutation causes a single amino acid substitution from Gly<sup>162 </sup>to Glu<sup>162</sup>, abolishing old3-1 OAS-TL activity <it>in vitro</it>. The <it>old3-1 </it>mutation segregates as a monogenic semi-dominant trait when backcrossed to its wild type accession Landsberg <it>erecta </it>(L<it>er</it>-0) and the Di-2 accession. Consistent with its semi-dominant behaviour, wild type L<it>er</it>-0 plants transformed with the mutated <it>old3-1 </it>gene, displayed the early leaf death phenotype. However, the <it>old3-1 </it>mutation segregates in an 11:4:1 (wild type: semi-dominant: mutant) ratio when backcrossed to the Colombia-0 and Wassilewskija accessions. Thus, the early leaf death phenotype depends on two semi-dominant loci. The second locus that determines the <it>old3-1 </it>early leaf death phenotype is referred to as <it>odd-ler </it>(for <it>old3 </it>determinant in the L<it>er </it>accession) and is located on chromosome 3. The early leaf death phenotype is temperature dependent and is associated with increased expression of defence-response and oxidative-stress marker genes. Independent of the presence of the <it>odd-ler </it>gene, <it>OAS-A1 </it>is involved in maintaining sulphur and thiol levels and is required for resistance against cadmium stress.</p> <p>Conclusions</p> <p>The cytosolic OAS-TL is involved in maintaining organic sulphur levels. The <it>old3-1 </it>mutation causes genome-dependent and independent phenotypes and uncovers a novel function for the mutated OAS-TL in cell death regulation.</p>http://www.biomedcentral.com/1471-2229/10/80 |
| spellingShingle | Schippers Jos HM Everts Gerja E Jing Hai-Chun Shirzadian-Khorramabad Reza Hille Jacques Dijkwel Paul P A mutation in the cytosolic O-acetylserine (thiol) lyase induces a genome-dependent early leaf death phenotype in <it>Arabidopsis</it> BMC Plant Biology |
| title | A mutation in the cytosolic O-acetylserine (thiol) lyase induces a genome-dependent early leaf death phenotype in <it>Arabidopsis</it> |
| title_full | A mutation in the cytosolic O-acetylserine (thiol) lyase induces a genome-dependent early leaf death phenotype in <it>Arabidopsis</it> |
| title_fullStr | A mutation in the cytosolic O-acetylserine (thiol) lyase induces a genome-dependent early leaf death phenotype in <it>Arabidopsis</it> |
| title_full_unstemmed | A mutation in the cytosolic O-acetylserine (thiol) lyase induces a genome-dependent early leaf death phenotype in <it>Arabidopsis</it> |
| title_short | A mutation in the cytosolic O-acetylserine (thiol) lyase induces a genome-dependent early leaf death phenotype in <it>Arabidopsis</it> |
| title_sort | mutation in the cytosolic o acetylserine thiol lyase induces a genome dependent early leaf death phenotype in it arabidopsis it |
| url | http://www.biomedcentral.com/1471-2229/10/80 |
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