The recovery of intestinal barrier function and changes in oral microbiota after radiation therapy injury
IntroductionColorectal cancer (CRC) is the third most common malignant tumor, and neoadjuvant chemo-radiotherapy is usually recommended for advanced stage colorectal cancer. Radiotherapy can cause damage to intestinal mucosal barrier, which may be related to perioperative complications. Intestinal m...
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| Format: | Article |
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1288666/full |
| _version_ | 1827356649906503680 |
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| author | Kun Wang Jingjing Zhang Yihao Zhang Junze Xue He Wang Xiaojie Tan Xuelong Jiao Haitao Jiang |
| author_facet | Kun Wang Jingjing Zhang Yihao Zhang Junze Xue He Wang Xiaojie Tan Xuelong Jiao Haitao Jiang |
| author_sort | Kun Wang |
| collection | DOAJ |
| description | IntroductionColorectal cancer (CRC) is the third most common malignant tumor, and neoadjuvant chemo-radiotherapy is usually recommended for advanced stage colorectal cancer. Radiotherapy can cause damage to intestinal mucosal barrier, which may be related to perioperative complications. Intestinal microbiota is one of the constituents of the intestinal mucosal biological barrier, and literature reports that patients with CRC have changes in corresponding oral microbiota. This study aims to analyze the levels of immunoglobulin SIgA, inflammatory factors, lymphocyte subsets quantity, and proportion in surgical specimens of intestinal mucosa at different time intervals after radiotherapy, in order to seek investigation for the optimal surgical time after radiotherapy and to provide evidence for finding probiotics or immunomodulators through high-throughput sequencing of bacterial 16s rRNA in patients' saliva microbiota. Ultimately, this may provide new ideas for reducing perioperative complications caused by radiotherapy-induced intestinal damage.MethodsWe selected intestinal mucosal tissue and saliva samples from over 40 patients in our center who did not undergo radiotherapy and underwent surgery at different time intervals after radiotherapy. Detection of SIgA was performed using ELISA assay. Western Blotting was used to detect IL-1β, IL-6, and IL-17 in the intestinal mucosal tissue. Flow cytometry was used to detect CD4 and CD8. And the microbial community changes in saliva samples were detected through 16s rRNA sequencing.ResultsAfter radiotherapy, changes in SIgA, various cytokines, CD4CD8 lymphocyte subsets, and oral microbiota in the intestinal mucosal tissue of rectal cancer patients may occur. Over time, this change may gradually recover.DiscussionIn colorectal cancer, oncological aspects often receive more attention, while studies focusing on the intestinal mucosal barrier are less common. This study aims to understand the repair mechanisms of the intestinal mucosal barrier and reduce complications arising from radiotherapy-induced damage. The relationship between oral microbiota and systemic diseases has gained interest in recent years. However, the literature on the oral microbiota after radiotherapy for rectal cancer remains scarce. This study addresses this gap by analysing changes in the salivary microbiota of rectal cancer patients before and after radiotherapy, shedding light on microbiota changes. It aims to lay the groundwork for identifying suitable probiotics or immunomodulators to alleviate perioperative complications and improve the prognosis of CRC. |
| first_indexed | 2024-03-08T05:11:57Z |
| format | Article |
| id | doaj.art-40119c456a0f47288c9f0e5fe6cfd46b |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-03-08T05:11:57Z |
| publishDate | 2024-02-01 |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-40119c456a0f47288c9f0e5fe6cfd46b2024-02-07T05:33:51ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882024-02-011310.3389/fcimb.2023.12886661288666The recovery of intestinal barrier function and changes in oral microbiota after radiation therapy injuryKun Wang0Jingjing Zhang1Yihao Zhang2Junze Xue3He Wang4Xiaojie Tan5Xuelong Jiao6Haitao Jiang7Department of Gastrointestinal Surgery, Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Pathology, Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Gastrointestinal Surgery, Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Gastrointestinal Surgery, Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Gastrointestinal Surgery, Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Gastrointestinal Surgery, Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Gastrointestinal Surgery, Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Gastrointestinal Surgery, Affiliated Hospital of Qingdao University, Qingdao, ChinaIntroductionColorectal cancer (CRC) is the third most common malignant tumor, and neoadjuvant chemo-radiotherapy is usually recommended for advanced stage colorectal cancer. Radiotherapy can cause damage to intestinal mucosal barrier, which may be related to perioperative complications. Intestinal microbiota is one of the constituents of the intestinal mucosal biological barrier, and literature reports that patients with CRC have changes in corresponding oral microbiota. This study aims to analyze the levels of immunoglobulin SIgA, inflammatory factors, lymphocyte subsets quantity, and proportion in surgical specimens of intestinal mucosa at different time intervals after radiotherapy, in order to seek investigation for the optimal surgical time after radiotherapy and to provide evidence for finding probiotics or immunomodulators through high-throughput sequencing of bacterial 16s rRNA in patients' saliva microbiota. Ultimately, this may provide new ideas for reducing perioperative complications caused by radiotherapy-induced intestinal damage.MethodsWe selected intestinal mucosal tissue and saliva samples from over 40 patients in our center who did not undergo radiotherapy and underwent surgery at different time intervals after radiotherapy. Detection of SIgA was performed using ELISA assay. Western Blotting was used to detect IL-1β, IL-6, and IL-17 in the intestinal mucosal tissue. Flow cytometry was used to detect CD4 and CD8. And the microbial community changes in saliva samples were detected through 16s rRNA sequencing.ResultsAfter radiotherapy, changes in SIgA, various cytokines, CD4CD8 lymphocyte subsets, and oral microbiota in the intestinal mucosal tissue of rectal cancer patients may occur. Over time, this change may gradually recover.DiscussionIn colorectal cancer, oncological aspects often receive more attention, while studies focusing on the intestinal mucosal barrier are less common. This study aims to understand the repair mechanisms of the intestinal mucosal barrier and reduce complications arising from radiotherapy-induced damage. The relationship between oral microbiota and systemic diseases has gained interest in recent years. However, the literature on the oral microbiota after radiotherapy for rectal cancer remains scarce. This study addresses this gap by analysing changes in the salivary microbiota of rectal cancer patients before and after radiotherapy, shedding light on microbiota changes. It aims to lay the groundwork for identifying suitable probiotics or immunomodulators to alleviate perioperative complications and improve the prognosis of CRC.https://www.frontiersin.org/articles/10.3389/fcimb.2023.1288666/full16s rRNA gene sequencingrectal cancer radiotherapyoral floraintestinal mucosal barrierrectal cancer |
| spellingShingle | Kun Wang Jingjing Zhang Yihao Zhang Junze Xue He Wang Xiaojie Tan Xuelong Jiao Haitao Jiang The recovery of intestinal barrier function and changes in oral microbiota after radiation therapy injury Frontiers in Cellular and Infection Microbiology 16s rRNA gene sequencing rectal cancer radiotherapy oral flora intestinal mucosal barrier rectal cancer |
| title | The recovery of intestinal barrier function and changes in oral microbiota after radiation therapy injury |
| title_full | The recovery of intestinal barrier function and changes in oral microbiota after radiation therapy injury |
| title_fullStr | The recovery of intestinal barrier function and changes in oral microbiota after radiation therapy injury |
| title_full_unstemmed | The recovery of intestinal barrier function and changes in oral microbiota after radiation therapy injury |
| title_short | The recovery of intestinal barrier function and changes in oral microbiota after radiation therapy injury |
| title_sort | recovery of intestinal barrier function and changes in oral microbiota after radiation therapy injury |
| topic | 16s rRNA gene sequencing rectal cancer radiotherapy oral flora intestinal mucosal barrier rectal cancer |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1288666/full |
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