Lactate transport and metabolism in rumen epithelial cells in SARA condition
Lactate, a main product of rumen microbial fermentation from dietary starch, has been recognised as an important mediator of rumen microbiota regulation in rumen acidosis. However, the mechanism of lactate transport and metabolism in the rumen remains unclear. This study aimed to evaluate the signif...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Italian Journal of Animal Science |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/1828051X.2023.2178340 |
| _version_ | 1827276561793941504 |
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| author | Banglin He Yaotian Fan Xiujuan Zhao Hongrong Wang |
| author_facet | Banglin He Yaotian Fan Xiujuan Zhao Hongrong Wang |
| author_sort | Banglin He |
| collection | DOAJ |
| description | Lactate, a main product of rumen microbial fermentation from dietary starch, has been recognised as an important mediator of rumen microbiota regulation in rumen acidosis. However, the mechanism of lactate transport and metabolism in the rumen remains unclear. This study aimed to evaluate the significance of the absorption, transport, and metabolism of lactic acid in the pH and LPS-induced subacute ruminal acidosis (SARA) model of rumen epithelial cells (RECs). In the current study, RECs were obtained from two adult Saanen goats (44.5 ± 4.6 kg BW) and using Lipopolysaccharide (LPS) and low pH to co-stimulate RECs to construct a microenvironment under SARA conditions, to further analyse the lactate transport and metabolism by RECs. The results showed that the LPS and low pH could significantly reduce cell viability by 29.36% (p < 0.05), induce inflammation in RECs, destroy tight junctions between RECs, and increase cell permeability. In addition, stimulation leads to changes in cellular metabolic activity and inhibits the expression of lactate transporters (p < 0.05), promoting an increased risk of lactate accumulation in the intracellular and intestinal lumen. This study hopefully provides a better understanding of lactate metabolic regulation and new perspectives for the nutritional prevention and treatment of SARA.HIGHLIGHTS Lactic acid is an important fermentation product in the rumen of ruminants, and its transport and metabolic regulation mechanisms are still unclear. To understand transport uptake in the rumen epithelium, a SARA model was therefore used. Low pH and high LPS trigger epithelial inflammation, increasing the risk of lactic acid entering the bloodstream. |
| first_indexed | 2024-03-08T16:59:46Z |
| format | Article |
| id | doaj.art-401390a08f6640288037c51fee1727fe |
| institution | Directory Open Access Journal |
| issn | 1594-4077 1828-051X |
| language | English |
| last_indexed | 2024-04-24T07:00:49Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Italian Journal of Animal Science |
| spelling | doaj.art-401390a08f6640288037c51fee1727fe2024-04-22T08:52:28ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2023-12-0122123924910.1080/1828051X.2023.21783402178340Lactate transport and metabolism in rumen epithelial cells in SARA conditionBanglin He0Yaotian Fan1Xiujuan Zhao2Hongrong Wang3Laboratory of Metabolic Manipulation of Herbivorous Animal Nutrition, College of Animal Science and Technology, Yangzhou UniversityLaboratory of Metabolic Manipulation of Herbivorous Animal Nutrition, College of Animal Science and Technology, Yangzhou UniversityLaboratory of Metabolic Manipulation of Herbivorous Animal Nutrition, College of Animal Science and Technology, Yangzhou UniversityLaboratory of Metabolic Manipulation of Herbivorous Animal Nutrition, College of Animal Science and Technology, Yangzhou UniversityLactate, a main product of rumen microbial fermentation from dietary starch, has been recognised as an important mediator of rumen microbiota regulation in rumen acidosis. However, the mechanism of lactate transport and metabolism in the rumen remains unclear. This study aimed to evaluate the significance of the absorption, transport, and metabolism of lactic acid in the pH and LPS-induced subacute ruminal acidosis (SARA) model of rumen epithelial cells (RECs). In the current study, RECs were obtained from two adult Saanen goats (44.5 ± 4.6 kg BW) and using Lipopolysaccharide (LPS) and low pH to co-stimulate RECs to construct a microenvironment under SARA conditions, to further analyse the lactate transport and metabolism by RECs. The results showed that the LPS and low pH could significantly reduce cell viability by 29.36% (p < 0.05), induce inflammation in RECs, destroy tight junctions between RECs, and increase cell permeability. In addition, stimulation leads to changes in cellular metabolic activity and inhibits the expression of lactate transporters (p < 0.05), promoting an increased risk of lactate accumulation in the intracellular and intestinal lumen. This study hopefully provides a better understanding of lactate metabolic regulation and new perspectives for the nutritional prevention and treatment of SARA.HIGHLIGHTS Lactic acid is an important fermentation product in the rumen of ruminants, and its transport and metabolic regulation mechanisms are still unclear. To understand transport uptake in the rumen epithelium, a SARA model was therefore used. Low pH and high LPS trigger epithelial inflammation, increasing the risk of lactic acid entering the bloodstream.http://dx.doi.org/10.1080/1828051X.2023.2178340subacute rumen acidosistransformationrumen epithelial cellsmonocarboxylate transportersgoats |
| spellingShingle | Banglin He Yaotian Fan Xiujuan Zhao Hongrong Wang Lactate transport and metabolism in rumen epithelial cells in SARA condition Italian Journal of Animal Science subacute rumen acidosis transformation rumen epithelial cells monocarboxylate transporters goats |
| title | Lactate transport and metabolism in rumen epithelial cells in SARA condition |
| title_full | Lactate transport and metabolism in rumen epithelial cells in SARA condition |
| title_fullStr | Lactate transport and metabolism in rumen epithelial cells in SARA condition |
| title_full_unstemmed | Lactate transport and metabolism in rumen epithelial cells in SARA condition |
| title_short | Lactate transport and metabolism in rumen epithelial cells in SARA condition |
| title_sort | lactate transport and metabolism in rumen epithelial cells in sara condition |
| topic | subacute rumen acidosis transformation rumen epithelial cells monocarboxylate transporters goats |
| url | http://dx.doi.org/10.1080/1828051X.2023.2178340 |
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