The Influence of Polyamines on the Expression of Escherichia Coli Ribosome Hibernation Factor Raia
RaiA is one of the main ribosome hibernation factors in Escherichia coli. Like other ribosome hibernation factors, this protein reversibly inhibits translation under stress conditions. According to published data, being induced by indole, RaiA is involved in bacterial persistence, which is considere...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2023-01-01
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| Series: | BIO Web of Conferences |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/pdf/2023/02/bioconf_itsm2023_02001.pdf |
| _version_ | 1797951222235267072 |
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| author | Khaova Elena A. Tkachenko Alexander G. |
| author_facet | Khaova Elena A. Tkachenko Alexander G. |
| author_sort | Khaova Elena A. |
| collection | DOAJ |
| description | RaiA is one of the main ribosome hibernation factors in Escherichia coli. Like other ribosome hibernation factors, this protein reversibly inhibits translation under stress conditions. According to published data, being induced by indole, RaiA is involved in bacterial persistence, which is considered to play important role in the recalcitrance of chronic infections to antibiotics. Previously, we showed that the raiA expression on the transcriptional level is stimulated by polyamines, in addition to indole. In this work, we investigated the influence of polyamines on the raiA expression on the translational level. We obtained the predicted secondary structures of raiA mRNA, the analysis of which showed the presence of the bulged-out region in the initiation site with a high probability. This may be a sign of gene involvement in the polyamine modulon. We constructed translational raiA::lacZ reporter fusion. Using this genetic construct, we studied the effects of polyamines on the raiA expression through an addition of putrescine, cadaverine or spermidine at concentrations of 1 mM and 2 mM. According to the results, the raiA expression is primarily stimulated by cadaverine at the stationary phase. |
| first_indexed | 2024-04-10T22:27:13Z |
| format | Article |
| id | doaj.art-4018759c540448a589ce249377b02b22 |
| institution | Directory Open Access Journal |
| issn | 2117-4458 |
| language | English |
| last_indexed | 2024-04-10T22:27:13Z |
| publishDate | 2023-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | BIO Web of Conferences |
| spelling | doaj.art-4018759c540448a589ce249377b02b222023-01-17T09:11:28ZengEDP SciencesBIO Web of Conferences2117-44582023-01-01570200110.1051/bioconf/20235702001bioconf_itsm2023_02001The Influence of Polyamines on the Expression of Escherichia Coli Ribosome Hibernation Factor RaiaKhaova Elena A.0Tkachenko Alexander G.1Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Center, the Ural Branch of Russian Academy of SciencesInstitute of Ecology and Genetics of Microorganisms, Perm Federal Research Center, the Ural Branch of Russian Academy of SciencesRaiA is one of the main ribosome hibernation factors in Escherichia coli. Like other ribosome hibernation factors, this protein reversibly inhibits translation under stress conditions. According to published data, being induced by indole, RaiA is involved in bacterial persistence, which is considered to play important role in the recalcitrance of chronic infections to antibiotics. Previously, we showed that the raiA expression on the transcriptional level is stimulated by polyamines, in addition to indole. In this work, we investigated the influence of polyamines on the raiA expression on the translational level. We obtained the predicted secondary structures of raiA mRNA, the analysis of which showed the presence of the bulged-out region in the initiation site with a high probability. This may be a sign of gene involvement in the polyamine modulon. We constructed translational raiA::lacZ reporter fusion. Using this genetic construct, we studied the effects of polyamines on the raiA expression through an addition of putrescine, cadaverine or spermidine at concentrations of 1 mM and 2 mM. According to the results, the raiA expression is primarily stimulated by cadaverine at the stationary phase.https://www.bio-conferences.org/articles/bioconf/pdf/2023/02/bioconf_itsm2023_02001.pdf |
| spellingShingle | Khaova Elena A. Tkachenko Alexander G. The Influence of Polyamines on the Expression of Escherichia Coli Ribosome Hibernation Factor Raia BIO Web of Conferences |
| title | The Influence of Polyamines on the Expression of Escherichia Coli Ribosome Hibernation Factor Raia |
| title_full | The Influence of Polyamines on the Expression of Escherichia Coli Ribosome Hibernation Factor Raia |
| title_fullStr | The Influence of Polyamines on the Expression of Escherichia Coli Ribosome Hibernation Factor Raia |
| title_full_unstemmed | The Influence of Polyamines on the Expression of Escherichia Coli Ribosome Hibernation Factor Raia |
| title_short | The Influence of Polyamines on the Expression of Escherichia Coli Ribosome Hibernation Factor Raia |
| title_sort | influence of polyamines on the expression of escherichia coli ribosome hibernation factor raia |
| url | https://www.bio-conferences.org/articles/bioconf/pdf/2023/02/bioconf_itsm2023_02001.pdf |
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