NK-92 cells retain vitality and functionality when grown in standard cell culture conditions
NK-92 cells are an off-the-shelf, cell-based immunotherapy currently in clinical trials for a variety of cancer types. As the most ‘NK-like’ cell line available, it is also an important research tool. To date, NK-92 cells have been cultivated in a costly and time-consumingly prepared specialized med...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8926178/?tool=EBI |
| _version_ | 1828877869330202624 |
|---|---|
| author | Rebecca Kotzur Alexandra Duev-Cohen Inbal Kol Adi Reches Ofer Mandelboim Natan Stein |
| author_facet | Rebecca Kotzur Alexandra Duev-Cohen Inbal Kol Adi Reches Ofer Mandelboim Natan Stein |
| author_sort | Rebecca Kotzur |
| collection | DOAJ |
| description | NK-92 cells are an off-the-shelf, cell-based immunotherapy currently in clinical trials for a variety of cancer types. As the most ‘NK-like’ cell line available, it is also an important research tool. To date, NK-92 cells have been cultivated in a costly and time-consumingly prepared specialized medium, complicating research with these cells. Here we show that NK-92 cells grow in the comparatively user-friendly RPMI medium supplemented with IL-2. We demonstrate that their metabolic activity and replication rates are even improved in RPMI. Furthermore, they can be grown in cell culture dishes and do not need to be expanded in ventilated flasks. We show that in RPMI the cells retain functional characteristics relating to receptor expression, IFN-γ secretion, and killing. Our findings will enable more researchers to work with and manipulate this cell line, hopefully leading to further discoveries and improved therapies. |
| first_indexed | 2024-12-13T08:53:29Z |
| format | Article |
| id | doaj.art-401963b8101f47fc94e660f5469724ec |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T08:53:29Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-401963b8101f47fc94e660f5469724ec2022-12-21T23:53:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01173NK-92 cells retain vitality and functionality when grown in standard cell culture conditionsRebecca KotzurAlexandra Duev-CohenInbal KolAdi RechesOfer MandelboimNatan SteinNK-92 cells are an off-the-shelf, cell-based immunotherapy currently in clinical trials for a variety of cancer types. As the most ‘NK-like’ cell line available, it is also an important research tool. To date, NK-92 cells have been cultivated in a costly and time-consumingly prepared specialized medium, complicating research with these cells. Here we show that NK-92 cells grow in the comparatively user-friendly RPMI medium supplemented with IL-2. We demonstrate that their metabolic activity and replication rates are even improved in RPMI. Furthermore, they can be grown in cell culture dishes and do not need to be expanded in ventilated flasks. We show that in RPMI the cells retain functional characteristics relating to receptor expression, IFN-γ secretion, and killing. Our findings will enable more researchers to work with and manipulate this cell line, hopefully leading to further discoveries and improved therapies.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8926178/?tool=EBI |
| spellingShingle | Rebecca Kotzur Alexandra Duev-Cohen Inbal Kol Adi Reches Ofer Mandelboim Natan Stein NK-92 cells retain vitality and functionality when grown in standard cell culture conditions PLoS ONE |
| title | NK-92 cells retain vitality and functionality when grown in standard cell culture conditions |
| title_full | NK-92 cells retain vitality and functionality when grown in standard cell culture conditions |
| title_fullStr | NK-92 cells retain vitality and functionality when grown in standard cell culture conditions |
| title_full_unstemmed | NK-92 cells retain vitality and functionality when grown in standard cell culture conditions |
| title_short | NK-92 cells retain vitality and functionality when grown in standard cell culture conditions |
| title_sort | nk 92 cells retain vitality and functionality when grown in standard cell culture conditions |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8926178/?tool=EBI |
| work_keys_str_mv | AT rebeccakotzur nk92cellsretainvitalityandfunctionalitywhengrowninstandardcellcultureconditions AT alexandraduevcohen nk92cellsretainvitalityandfunctionalitywhengrowninstandardcellcultureconditions AT inbalkol nk92cellsretainvitalityandfunctionalitywhengrowninstandardcellcultureconditions AT adireches nk92cellsretainvitalityandfunctionalitywhengrowninstandardcellcultureconditions AT ofermandelboim nk92cellsretainvitalityandfunctionalitywhengrowninstandardcellcultureconditions AT natanstein nk92cellsretainvitalityandfunctionalitywhengrowninstandardcellcultureconditions |