The New <i>Klebsiella</i> <i>pneumoniae</i> ST152 Variants with Hypermucoviscous Phenotype Isolated from Renal Transplant Recipients with Asymptomatic Bacteriuria—Genetic Characteristics by WGS
<i>Klebsiella pneumoniae</i> (Kp) is one of the most important etiological factors of urinary tract infections in renal transplant (RTx) recipients. We described the antimicrobial susceptibility phenotypes and genomic features of two hypermucoviscous (HM) Kp isolates recovered from RTx r...
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MDPI AG
2020-10-01
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| author | Magdalena Wysocka Roxana Zamudio Marco R Oggioni Justyna Gołębiewska Aleksandra Dudziak Beata Krawczyk |
| author_facet | Magdalena Wysocka Roxana Zamudio Marco R Oggioni Justyna Gołębiewska Aleksandra Dudziak Beata Krawczyk |
| author_sort | Magdalena Wysocka |
| collection | DOAJ |
| description | <i>Klebsiella pneumoniae</i> (Kp) is one of the most important etiological factors of urinary tract infections in renal transplant (RTx) recipients. We described the antimicrobial susceptibility phenotypes and genomic features of two hypermucoviscous (HM) Kp isolates recovered from RTx recipients with asymptomatic bacteriuria (ABU). Using whole genome sequencing (WGS) data, we showed that the strains belong to the ST152 lineage with the KL149 capsular serotype, but without <i>rmp</i>A/<i>mag</i>A genes, which is typical for HM+ hypervirulent Kp. These new strains carried virulence-associated genes that predispose for urinary tract infections (UTIs). Likewise, both strains carried the <i>ecp</i> gene encoding pilus common for extended-spectrum β-lactamase (ESBL) <i>Escherichia coli</i>. Although the two ST152 isolates were closely related and differed by only nine single nucleotide polymorphisms (SNPs) in their chromosomes, they had different plasmid compositions and chromosomal elements, with isolate KP28872 carrying an ESBL plasmid and an integrative conjugative element. These two isolates are an example of the high plasticity of the <i>K. pneumoniae</i> accessory genome. The identification of patients with ABU matched with the correct epidemiological profiling of isolates could facilitate interventions to prevent or rapidly treat <i>K. pneumoniae</i> infections. |
| first_indexed | 2024-03-10T15:40:17Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T15:40:17Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-40201bde8c4e4c6d8b6fc06135b9725a2023-11-20T16:53:46ZengMDPI AGGenes2073-44252020-10-011110118910.3390/genes11101189The New <i>Klebsiella</i> <i>pneumoniae</i> ST152 Variants with Hypermucoviscous Phenotype Isolated from Renal Transplant Recipients with Asymptomatic Bacteriuria—Genetic Characteristics by WGSMagdalena Wysocka0Roxana Zamudio1Marco R Oggioni2Justyna Gołębiewska3Aleksandra Dudziak4Beata Krawczyk5Department of Molecular Biotechnology and Microbiology, Faculty of Chemistry, Gdańsk University of Technology, ul. Narutowicza 11/12, 80-233 Gdańsk, PolandDepartment of Genetics and Genome Biology, University of Leicester, University Road, Leicester LE1 7RH, UKDepartment of Genetics and Genome Biology, University of Leicester, University Road, Leicester LE1 7RH, UKDepartment of Nephrology, Transplantology and Internal Medicine, Medical University of Gdańsk, ul. Dębinki 7, 80-952 Gdańsk, PolandLaboratory of Clinical Microbiology, University Centre for Laboratory Diagnostics, Medical University of Gdańsk Clinical Centre, ul. Dębinki 7, 80-952 Gdańsk, PolandDepartment of Molecular Biotechnology and Microbiology, Faculty of Chemistry, Gdańsk University of Technology, ul. Narutowicza 11/12, 80-233 Gdańsk, Poland<i>Klebsiella pneumoniae</i> (Kp) is one of the most important etiological factors of urinary tract infections in renal transplant (RTx) recipients. We described the antimicrobial susceptibility phenotypes and genomic features of two hypermucoviscous (HM) Kp isolates recovered from RTx recipients with asymptomatic bacteriuria (ABU). Using whole genome sequencing (WGS) data, we showed that the strains belong to the ST152 lineage with the KL149 capsular serotype, but without <i>rmp</i>A/<i>mag</i>A genes, which is typical for HM+ hypervirulent Kp. These new strains carried virulence-associated genes that predispose for urinary tract infections (UTIs). Likewise, both strains carried the <i>ecp</i> gene encoding pilus common for extended-spectrum β-lactamase (ESBL) <i>Escherichia coli</i>. Although the two ST152 isolates were closely related and differed by only nine single nucleotide polymorphisms (SNPs) in their chromosomes, they had different plasmid compositions and chromosomal elements, with isolate KP28872 carrying an ESBL plasmid and an integrative conjugative element. These two isolates are an example of the high plasticity of the <i>K. pneumoniae</i> accessory genome. The identification of patients with ABU matched with the correct epidemiological profiling of isolates could facilitate interventions to prevent or rapidly treat <i>K. pneumoniae</i> infections.https://www.mdpi.com/2073-4425/11/10/1189renal transplantABU<i>Klebsiella pneumoniae</i>urinary tract infectionwhole genome sequencinghorizontal gene transfer |
| spellingShingle | Magdalena Wysocka Roxana Zamudio Marco R Oggioni Justyna Gołębiewska Aleksandra Dudziak Beata Krawczyk The New <i>Klebsiella</i> <i>pneumoniae</i> ST152 Variants with Hypermucoviscous Phenotype Isolated from Renal Transplant Recipients with Asymptomatic Bacteriuria—Genetic Characteristics by WGS Genes renal transplant ABU <i>Klebsiella pneumoniae</i> urinary tract infection whole genome sequencing horizontal gene transfer |
| title | The New <i>Klebsiella</i> <i>pneumoniae</i> ST152 Variants with Hypermucoviscous Phenotype Isolated from Renal Transplant Recipients with Asymptomatic Bacteriuria—Genetic Characteristics by WGS |
| title_full | The New <i>Klebsiella</i> <i>pneumoniae</i> ST152 Variants with Hypermucoviscous Phenotype Isolated from Renal Transplant Recipients with Asymptomatic Bacteriuria—Genetic Characteristics by WGS |
| title_fullStr | The New <i>Klebsiella</i> <i>pneumoniae</i> ST152 Variants with Hypermucoviscous Phenotype Isolated from Renal Transplant Recipients with Asymptomatic Bacteriuria—Genetic Characteristics by WGS |
| title_full_unstemmed | The New <i>Klebsiella</i> <i>pneumoniae</i> ST152 Variants with Hypermucoviscous Phenotype Isolated from Renal Transplant Recipients with Asymptomatic Bacteriuria—Genetic Characteristics by WGS |
| title_short | The New <i>Klebsiella</i> <i>pneumoniae</i> ST152 Variants with Hypermucoviscous Phenotype Isolated from Renal Transplant Recipients with Asymptomatic Bacteriuria—Genetic Characteristics by WGS |
| title_sort | new i klebsiella i i pneumoniae i st152 variants with hypermucoviscous phenotype isolated from renal transplant recipients with asymptomatic bacteriuria genetic characteristics by wgs |
| topic | renal transplant ABU <i>Klebsiella pneumoniae</i> urinary tract infection whole genome sequencing horizontal gene transfer |
| url | https://www.mdpi.com/2073-4425/11/10/1189 |
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