Hyperoside and Quercitrin in <i>Houttuynia cordata</i> Extract Attenuate UVB-Induced Human Keratinocyte Cell Damage and Oxidative Stress via Modulation of MAPKs and Akt Signaling Pathway
Ultraviolet radiation is a major environmental harmful factor on human skin. In this paper, we investigate the potential mechanism of <i>Houttuynia cordata</i> extract on UVB-induced HaCaT keratinocyte cell death and inflammation. We found that <i>Houttuynia cordata</i> ethyl...
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MDPI AG
2022-01-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/2/221 |
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| author | Nattakan Charachit Amonnat Sukhamwang Pornngarm Dejkriengkraikul Supachai Yodkeeree |
| author_facet | Nattakan Charachit Amonnat Sukhamwang Pornngarm Dejkriengkraikul Supachai Yodkeeree |
| author_sort | Nattakan Charachit |
| collection | DOAJ |
| description | Ultraviolet radiation is a major environmental harmful factor on human skin. In this paper, we investigate the potential mechanism of <i>Houttuynia cordata</i> extract on UVB-induced HaCaT keratinocyte cell death and inflammation. We found that <i>Houttuynia cordata</i> ethyl acetate extract fraction (HC-EA) protected against UVB-induced cell damage. The HPLC results indicate that quercitrin and hyperoside are the major polyphenolics in HC-EA and are responsible for providing protection against UVB-induced cell death. These responses were associated with the regulation of caspase-9 and caspase-3 activation, which rescued HaCaT cells from UVB-induced apoptosis. In addition, HC-EA, quercitrin, and hyperoside attenuated UVB-induced inflammatory mediators, including IL-6, IL-8, COX-2, and iNOS. Furthermore, the treatment of cells with HC-EA and its active compounds abolished intracellular ROS and increased levels of heme oxygenase-1 and superoxide dismutase. UVB-induced ROS production mediated Akt and mitogen activated protein kinases (MAPKs) pathways, including p38, ERK, and JNK. Our results show HC-EA, quercitrin, and hyperoside decreased UVB-induced p38 and JNK phosphorylation, while increasing ERK and Akt phosphorylation. MAPKs and Akt mediated cell survival and death were confirmed by specific inhibitors to Akt and MAPKs. Thus, HC-EA, which contains quercitrin and hyperoside, protected keratinocyte from UVB-induced oxidative damage and inflammation through the modulation of MAPKs and Akt signaling. |
| first_indexed | 2024-03-09T22:45:20Z |
| format | Article |
| id | doaj.art-402332df7324488ebc885dae0e1f3c54 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T22:45:20Z |
| publishDate | 2022-01-01 |
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| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-402332df7324488ebc885dae0e1f3c542023-11-23T18:30:18ZengMDPI AGAntioxidants2076-39212022-01-0111222110.3390/antiox11020221Hyperoside and Quercitrin in <i>Houttuynia cordata</i> Extract Attenuate UVB-Induced Human Keratinocyte Cell Damage and Oxidative Stress via Modulation of MAPKs and Akt Signaling PathwayNattakan Charachit0Amonnat Sukhamwang1Pornngarm Dejkriengkraikul2Supachai Yodkeeree3Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandUltraviolet radiation is a major environmental harmful factor on human skin. In this paper, we investigate the potential mechanism of <i>Houttuynia cordata</i> extract on UVB-induced HaCaT keratinocyte cell death and inflammation. We found that <i>Houttuynia cordata</i> ethyl acetate extract fraction (HC-EA) protected against UVB-induced cell damage. The HPLC results indicate that quercitrin and hyperoside are the major polyphenolics in HC-EA and are responsible for providing protection against UVB-induced cell death. These responses were associated with the regulation of caspase-9 and caspase-3 activation, which rescued HaCaT cells from UVB-induced apoptosis. In addition, HC-EA, quercitrin, and hyperoside attenuated UVB-induced inflammatory mediators, including IL-6, IL-8, COX-2, and iNOS. Furthermore, the treatment of cells with HC-EA and its active compounds abolished intracellular ROS and increased levels of heme oxygenase-1 and superoxide dismutase. UVB-induced ROS production mediated Akt and mitogen activated protein kinases (MAPKs) pathways, including p38, ERK, and JNK. Our results show HC-EA, quercitrin, and hyperoside decreased UVB-induced p38 and JNK phosphorylation, while increasing ERK and Akt phosphorylation. MAPKs and Akt mediated cell survival and death were confirmed by specific inhibitors to Akt and MAPKs. Thus, HC-EA, which contains quercitrin and hyperoside, protected keratinocyte from UVB-induced oxidative damage and inflammation through the modulation of MAPKs and Akt signaling.https://www.mdpi.com/2076-3921/11/2/221<i>Houttuynia cordata</i>UVBantioxidantanti-apoptosisHaCaT keratinocyte |
| spellingShingle | Nattakan Charachit Amonnat Sukhamwang Pornngarm Dejkriengkraikul Supachai Yodkeeree Hyperoside and Quercitrin in <i>Houttuynia cordata</i> Extract Attenuate UVB-Induced Human Keratinocyte Cell Damage and Oxidative Stress via Modulation of MAPKs and Akt Signaling Pathway Antioxidants <i>Houttuynia cordata</i> UVB antioxidant anti-apoptosis HaCaT keratinocyte |
| title | Hyperoside and Quercitrin in <i>Houttuynia cordata</i> Extract Attenuate UVB-Induced Human Keratinocyte Cell Damage and Oxidative Stress via Modulation of MAPKs and Akt Signaling Pathway |
| title_full | Hyperoside and Quercitrin in <i>Houttuynia cordata</i> Extract Attenuate UVB-Induced Human Keratinocyte Cell Damage and Oxidative Stress via Modulation of MAPKs and Akt Signaling Pathway |
| title_fullStr | Hyperoside and Quercitrin in <i>Houttuynia cordata</i> Extract Attenuate UVB-Induced Human Keratinocyte Cell Damage and Oxidative Stress via Modulation of MAPKs and Akt Signaling Pathway |
| title_full_unstemmed | Hyperoside and Quercitrin in <i>Houttuynia cordata</i> Extract Attenuate UVB-Induced Human Keratinocyte Cell Damage and Oxidative Stress via Modulation of MAPKs and Akt Signaling Pathway |
| title_short | Hyperoside and Quercitrin in <i>Houttuynia cordata</i> Extract Attenuate UVB-Induced Human Keratinocyte Cell Damage and Oxidative Stress via Modulation of MAPKs and Akt Signaling Pathway |
| title_sort | hyperoside and quercitrin in i houttuynia cordata i extract attenuate uvb induced human keratinocyte cell damage and oxidative stress via modulation of mapks and akt signaling pathway |
| topic | <i>Houttuynia cordata</i> UVB antioxidant anti-apoptosis HaCaT keratinocyte |
| url | https://www.mdpi.com/2076-3921/11/2/221 |
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