Multi-tissue scRNA-seq reveals immune cell landscape of turbot (Scophthalmus maximus)
In vertebrates, bony fishes possess not only innate immune cells but also T and B cells that are equivalent to those in mammals. However, the precise sub-cluster of immune cells in teleost fish remains largely unknown. Herein, we developed a dynamic bacterial infection model in turbot (Scophthalmus...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co. Ltd.
2022-07-01
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| Series: | Fundamental Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667325822000668 |
| _version_ | 1828085990361661440 |
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| author | Weijie Chen Jianchang Huang Wei Wang Ying Wang Hao Chen Qiyao Wang Yuanxing Zhang Qin Liu Dahai Yang |
| author_facet | Weijie Chen Jianchang Huang Wei Wang Ying Wang Hao Chen Qiyao Wang Yuanxing Zhang Qin Liu Dahai Yang |
| author_sort | Weijie Chen |
| collection | DOAJ |
| description | In vertebrates, bony fishes possess not only innate immune cells but also T and B cells that are equivalent to those in mammals. However, the precise sub-cluster of immune cells in teleost fish remains largely unknown. Herein, we developed a dynamic bacterial infection model in turbot (Scophthalmus maximus) and created a fish immune cell landscape (FICL) for a primary lymphoid organ (head kidney), a secondary lymphoid organ (spleen), and barrier tissues (gills and posterior intestine). Moreover, through comprehensive characterization of the expression profiles of 16 clusters, including dendritic cells-like (DCs-like), macrophages (MΦs), neutrophils, NK cells, as well as 12 sub-clusters of T and B cells, we found that CD8+ CTLs, CD4−CD8− T, Th17 and ILC3-2 like cells possess a bifunctional role associated with cytotoxicity and immunoregulation during bacterial infection. To our knowledge, these results could provide a useful resource for a better understanding of immune cells in teleost fish and could act as a comprehensive knowledge base for assessing the evolutionary mechanism of adaptive immunity in vertebrates. |
| first_indexed | 2024-04-11T04:48:40Z |
| format | Article |
| id | doaj.art-4027b603b7d74cd490c40eae79ab2adc |
| institution | Directory Open Access Journal |
| issn | 2667-3258 |
| language | English |
| last_indexed | 2024-04-11T04:48:40Z |
| publishDate | 2022-07-01 |
| publisher | KeAi Communications Co. Ltd. |
| record_format | Article |
| series | Fundamental Research |
| spelling | doaj.art-4027b603b7d74cd490c40eae79ab2adc2022-12-27T04:43:11ZengKeAi Communications Co. Ltd.Fundamental Research2667-32582022-07-0124550561Multi-tissue scRNA-seq reveals immune cell landscape of turbot (Scophthalmus maximus)Weijie Chen0Jianchang Huang1Wei Wang2Ying Wang3Hao Chen4Qiyao Wang5Yuanxing Zhang6Qin Liu7Dahai Yang8State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, ChinaState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, ChinaState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, ChinaState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, ChinaState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, ChinaState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, ChinaSouthern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519000, China; Shanghai Engineering Research Center of Maricultured Animal Vaccines, Shanghai 200237, ChinaState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China; Corresponding authors.State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; Shanghai Engineering Research Center of Maricultured Animal Vaccines, Shanghai 200237, China; Corresponding authors.In vertebrates, bony fishes possess not only innate immune cells but also T and B cells that are equivalent to those in mammals. However, the precise sub-cluster of immune cells in teleost fish remains largely unknown. Herein, we developed a dynamic bacterial infection model in turbot (Scophthalmus maximus) and created a fish immune cell landscape (FICL) for a primary lymphoid organ (head kidney), a secondary lymphoid organ (spleen), and barrier tissues (gills and posterior intestine). Moreover, through comprehensive characterization of the expression profiles of 16 clusters, including dendritic cells-like (DCs-like), macrophages (MΦs), neutrophils, NK cells, as well as 12 sub-clusters of T and B cells, we found that CD8+ CTLs, CD4−CD8− T, Th17 and ILC3-2 like cells possess a bifunctional role associated with cytotoxicity and immunoregulation during bacterial infection. To our knowledge, these results could provide a useful resource for a better understanding of immune cells in teleost fish and could act as a comprehensive knowledge base for assessing the evolutionary mechanism of adaptive immunity in vertebrates.http://www.sciencedirect.com/science/article/pii/S2667325822000668ScRNA-seqFish immune cell landscapeDynamic immune cell responsesEdwardsiella piscicida infectionTurbot |
| spellingShingle | Weijie Chen Jianchang Huang Wei Wang Ying Wang Hao Chen Qiyao Wang Yuanxing Zhang Qin Liu Dahai Yang Multi-tissue scRNA-seq reveals immune cell landscape of turbot (Scophthalmus maximus) Fundamental Research ScRNA-seq Fish immune cell landscape Dynamic immune cell responses Edwardsiella piscicida infection Turbot |
| title | Multi-tissue scRNA-seq reveals immune cell landscape of turbot (Scophthalmus maximus) |
| title_full | Multi-tissue scRNA-seq reveals immune cell landscape of turbot (Scophthalmus maximus) |
| title_fullStr | Multi-tissue scRNA-seq reveals immune cell landscape of turbot (Scophthalmus maximus) |
| title_full_unstemmed | Multi-tissue scRNA-seq reveals immune cell landscape of turbot (Scophthalmus maximus) |
| title_short | Multi-tissue scRNA-seq reveals immune cell landscape of turbot (Scophthalmus maximus) |
| title_sort | multi tissue scrna seq reveals immune cell landscape of turbot scophthalmus maximus |
| topic | ScRNA-seq Fish immune cell landscape Dynamic immune cell responses Edwardsiella piscicida infection Turbot |
| url | http://www.sciencedirect.com/science/article/pii/S2667325822000668 |
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