Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production
Product yield on carbohydrate feedstocks is a key performance indicator for industrial ethanol production with the yeast Saccharomyces cerevisiae. This paper reviews pathway engineering strategies for improving ethanol yield on glucose and/or sucrose in anaerobic cultures of this yeast by altering t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2022-03-01
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| Series: | Synthetic and Systems Biotechnology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405805X21000995 |
| _version_ | 1797205345509048320 |
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| author | Aafke C.A. van Aalst Sophie C. de Valk Walter M. van Gulik Mickel L.A. Jansen Jack T. Pronk Robert Mans |
| author_facet | Aafke C.A. van Aalst Sophie C. de Valk Walter M. van Gulik Mickel L.A. Jansen Jack T. Pronk Robert Mans |
| author_sort | Aafke C.A. van Aalst |
| collection | DOAJ |
| description | Product yield on carbohydrate feedstocks is a key performance indicator for industrial ethanol production with the yeast Saccharomyces cerevisiae. This paper reviews pathway engineering strategies for improving ethanol yield on glucose and/or sucrose in anaerobic cultures of this yeast by altering the ratio of ethanol production, yeast growth and glycerol formation. Particular attention is paid to strategies aimed at altering energy coupling of alcoholic fermentation and to strategies for altering redox-cofactor coupling in carbon and nitrogen metabolism that aim to reduce or eliminate the role of glycerol formation in anaerobic redox metabolism. In addition to providing an overview of scientific advances we discuss context dependency, theoretical impact and potential for industrial application of different proposed and developed strategies. |
| first_indexed | 2024-04-24T08:49:39Z |
| format | Article |
| id | doaj.art-4028fff629bf44c2b97cdda63b541213 |
| institution | Directory Open Access Journal |
| issn | 2405-805X |
| language | English |
| last_indexed | 2024-04-24T08:49:39Z |
| publishDate | 2022-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Synthetic and Systems Biotechnology |
| spelling | doaj.art-4028fff629bf44c2b97cdda63b5412132024-04-16T12:29:06ZengKeAi Communications Co., Ltd.Synthetic and Systems Biotechnology2405-805X2022-03-0171554566Pathway engineering strategies for improved product yield in yeast-based industrial ethanol productionAafke C.A. van Aalst0Sophie C. de Valk1Walter M. van Gulik2Mickel L.A. Jansen3Jack T. Pronk4Robert Mans5Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629, HZ Delft, the NetherlandsDepartment of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629, HZ Delft, the NetherlandsDepartment of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629, HZ Delft, the NetherlandsDSM Biotechnology Centre, Alexander Fleminglaan 1, 2613, AX Delft, the NetherlandsDepartment of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629, HZ Delft, the NetherlandsDepartment of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629, HZ Delft, the Netherlands; Corresponding author.Product yield on carbohydrate feedstocks is a key performance indicator for industrial ethanol production with the yeast Saccharomyces cerevisiae. This paper reviews pathway engineering strategies for improving ethanol yield on glucose and/or sucrose in anaerobic cultures of this yeast by altering the ratio of ethanol production, yeast growth and glycerol formation. Particular attention is paid to strategies aimed at altering energy coupling of alcoholic fermentation and to strategies for altering redox-cofactor coupling in carbon and nitrogen metabolism that aim to reduce or eliminate the role of glycerol formation in anaerobic redox metabolism. In addition to providing an overview of scientific advances we discuss context dependency, theoretical impact and potential for industrial application of different proposed and developed strategies.http://www.sciencedirect.com/science/article/pii/S2405805X21000995Saccharomyces cerevisiaeSynthetic biologyMetabolic engineeringBiofuelsRedox metabolismEnergy metabolism |
| spellingShingle | Aafke C.A. van Aalst Sophie C. de Valk Walter M. van Gulik Mickel L.A. Jansen Jack T. Pronk Robert Mans Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production Synthetic and Systems Biotechnology Saccharomyces cerevisiae Synthetic biology Metabolic engineering Biofuels Redox metabolism Energy metabolism |
| title | Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production |
| title_full | Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production |
| title_fullStr | Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production |
| title_full_unstemmed | Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production |
| title_short | Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production |
| title_sort | pathway engineering strategies for improved product yield in yeast based industrial ethanol production |
| topic | Saccharomyces cerevisiae Synthetic biology Metabolic engineering Biofuels Redox metabolism Energy metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S2405805X21000995 |
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