Validation of a new method for the detection of Ethyl glucuronide in larvae of Lucilia sericata as a marker of ante-mortem alcohol consumption
Larvae and insects are an important and alternative biological matrix in the development anpost-mortem forensic toxicology. They are very useful when conventional matrices are not available, in particular when the loss of biological fluids, due to the decomposition of corpses or to a traumatic death...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023080106 |
| _version_ | 1797646438659784704 |
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| author | Alice Cerioni Erika Buratti Gianmario Mietti Marta Cippitelli Mariano Cingolani Rino Froldi Roberto Scendoni |
| author_facet | Alice Cerioni Erika Buratti Gianmario Mietti Marta Cippitelli Mariano Cingolani Rino Froldi Roberto Scendoni |
| author_sort | Alice Cerioni |
| collection | DOAJ |
| description | Larvae and insects are an important and alternative biological matrix in the development anpost-mortem forensic toxicology. They are very useful when conventional matrices are not available, in particular when the loss of biological fluids, due to the decomposition of corpses or to a traumatic death, occurs. The purpose of this study is to develop and validate an analytical method in Ultra High-Performance Liquid Chromatography at High Resolution Mass Spectrometry (HPLC/HR-MS) for the research and quantification of Ethyl glucuronide (EtG) on larvae. The criteria taken into consideration for the validation are linearity, quantitation limits (LOD and LLOQ), accuracy, precision, carryover, interferences and ionization suppression/enhancement. The method was shown to be linear within the tested range, with a coefficient of determination higher than 0.99. LOD was 2 pg mg−1, while LLOQ was 5 pg mg-1. Accuracy, precision and ionization/suppression enhancement fulfilled the criteria indicated in the guidelines used for the validation. The establishment and validation of this method allowed the identification of Ethyl glucuronide on the larvae of Lucilia sericata (Calliphoridae) of a subject found death in an advanced state of decomposition. |
| first_indexed | 2024-03-11T15:02:37Z |
| format | Article |
| id | doaj.art-4029f7dc2a424592846e914f4a64702f |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-11T15:02:37Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-4029f7dc2a424592846e914f4a64702f2023-10-30T06:07:28ZengElsevierHeliyon2405-84402023-10-01910e20802Validation of a new method for the detection of Ethyl glucuronide in larvae of Lucilia sericata as a marker of ante-mortem alcohol consumptionAlice Cerioni0Erika Buratti1Gianmario Mietti2Marta Cippitelli3Mariano Cingolani4Rino Froldi5Roberto Scendoni6Forensic Medicine Laboratory, Institute of Legal Medicine, University of Macerata, Via Don Minzoni 9, 62100 Macerata, ItalyForensic Medicine Laboratory, Institute of Legal Medicine, University of Macerata, Via Don Minzoni 9, 62100 Macerata, ItalyCorresponding author. Via Giovanni XXII, 45, 62100 Macerata, Italy.; Forensic Medicine Laboratory, Institute of Legal Medicine, University of Macerata, Via Don Minzoni 9, 62100 Macerata, ItalyForensic Medicine Laboratory, Institute of Legal Medicine, University of Macerata, Via Don Minzoni 9, 62100 Macerata, ItalyForensic Medicine Laboratory, Institute of Legal Medicine, University of Macerata, Via Don Minzoni 9, 62100 Macerata, ItalyForensic Medicine Laboratory, Institute of Legal Medicine, University of Macerata, Via Don Minzoni 9, 62100 Macerata, ItalyForensic Medicine Laboratory, Institute of Legal Medicine, University of Macerata, Via Don Minzoni 9, 62100 Macerata, ItalyLarvae and insects are an important and alternative biological matrix in the development anpost-mortem forensic toxicology. They are very useful when conventional matrices are not available, in particular when the loss of biological fluids, due to the decomposition of corpses or to a traumatic death, occurs. The purpose of this study is to develop and validate an analytical method in Ultra High-Performance Liquid Chromatography at High Resolution Mass Spectrometry (HPLC/HR-MS) for the research and quantification of Ethyl glucuronide (EtG) on larvae. The criteria taken into consideration for the validation are linearity, quantitation limits (LOD and LLOQ), accuracy, precision, carryover, interferences and ionization suppression/enhancement. The method was shown to be linear within the tested range, with a coefficient of determination higher than 0.99. LOD was 2 pg mg−1, while LLOQ was 5 pg mg-1. Accuracy, precision and ionization/suppression enhancement fulfilled the criteria indicated in the guidelines used for the validation. The establishment and validation of this method allowed the identification of Ethyl glucuronide on the larvae of Lucilia sericata (Calliphoridae) of a subject found death in an advanced state of decomposition.http://www.sciencedirect.com/science/article/pii/S2405844023080106EntomotoxicologyEthanolForensic toxicologyEthyl glucuronideUltra-high-performance liquid cromatographyLarvae |
| spellingShingle | Alice Cerioni Erika Buratti Gianmario Mietti Marta Cippitelli Mariano Cingolani Rino Froldi Roberto Scendoni Validation of a new method for the detection of Ethyl glucuronide in larvae of Lucilia sericata as a marker of ante-mortem alcohol consumption Heliyon Entomotoxicology Ethanol Forensic toxicology Ethyl glucuronide Ultra-high-performance liquid cromatography Larvae |
| title | Validation of a new method for the detection of Ethyl glucuronide in larvae of Lucilia sericata as a marker of ante-mortem alcohol consumption |
| title_full | Validation of a new method for the detection of Ethyl glucuronide in larvae of Lucilia sericata as a marker of ante-mortem alcohol consumption |
| title_fullStr | Validation of a new method for the detection of Ethyl glucuronide in larvae of Lucilia sericata as a marker of ante-mortem alcohol consumption |
| title_full_unstemmed | Validation of a new method for the detection of Ethyl glucuronide in larvae of Lucilia sericata as a marker of ante-mortem alcohol consumption |
| title_short | Validation of a new method for the detection of Ethyl glucuronide in larvae of Lucilia sericata as a marker of ante-mortem alcohol consumption |
| title_sort | validation of a new method for the detection of ethyl glucuronide in larvae of lucilia sericata as a marker of ante mortem alcohol consumption |
| topic | Entomotoxicology Ethanol Forensic toxicology Ethyl glucuronide Ultra-high-performance liquid cromatography Larvae |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023080106 |
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