Transcriptome Analysis Reveals the Gene Expression Changes in the Silkworm (<i>Bombyx mori</i>) in Response to Hydrogen Sulfide Exposure
Hydrogen sulfide (H<sub>2</sub>S) has been recognized for its beneficial influence on physiological alterations. The development (body weight) and economic characteristics (cocoon weight, cocoon shell ratio, and cocoon shell weight) of silkworms were increased after continuous 7.5 µM H&l...
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MDPI AG
2021-12-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/12/12/1110 |
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| author | Rui Zhang Yu-Yao Cao Juan Du Kiran Thakur Shun-Ming Tang Fei Hu Zhao-Jun Wei |
| author_facet | Rui Zhang Yu-Yao Cao Juan Du Kiran Thakur Shun-Ming Tang Fei Hu Zhao-Jun Wei |
| author_sort | Rui Zhang |
| collection | DOAJ |
| description | Hydrogen sulfide (H<sub>2</sub>S) has been recognized for its beneficial influence on physiological alterations. The development (body weight) and economic characteristics (cocoon weight, cocoon shell ratio, and cocoon shell weight) of silkworms were increased after continuous 7.5 µM H<sub>2</sub>S treatment. In the present study, gene expression changes in the fat body of silkworms at the 5th instar larvae in response to the H<sub>2</sub>S were investigated through comparative transcriptome analysis. Moreover, the expression pattern of significant differentially expressed genes (DEGs) at the 5th instar larvae was confirmed by quantitative real-time PCR (qRT-PCR) after H<sub>2</sub>S exposure. A total of 1200 (DEGs) was identified, of which 977 DEGs were up-regulated and 223 DEGs were down-regulated. Most of the DEGs were involved in the transport pathway, cellular community, carbohydrate metabolism, and immune-associated signal transduction. The up regulated genes under H<sub>2</sub>S exposure were involved in endocytosis, glycolysis/gluconeogenesis, the citrate cycle (TCA cycle), and the synthesis of fibroin, while genes related to inflammation were down-regulated, indicating that H<sub>2</sub>S could promote energy metabolism, the transport pathway, silk synthesis, and inhibit inflammation in the silkworm. In addition, the expression levels of these genes were increased or decreased in a time-dependent manner during the 5th instar larvae. These results provided insight into the effects of H<sub>2</sub>S on silkworms at the transcriptional level and a substantial foundation for understanding H<sub>2</sub>S function. |
| first_indexed | 2024-03-10T03:52:32Z |
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| language | English |
| last_indexed | 2024-03-10T03:52:32Z |
| publishDate | 2021-12-01 |
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| series | Insects |
| spelling | doaj.art-402ee1cc2fc8461593901408decf8d352023-11-23T08:53:01ZengMDPI AGInsects2075-44502021-12-011212111010.3390/insects12121110Transcriptome Analysis Reveals the Gene Expression Changes in the Silkworm (<i>Bombyx mori</i>) in Response to Hydrogen Sulfide ExposureRui Zhang0Yu-Yao Cao1Juan Du2Kiran Thakur3Shun-Ming Tang4Fei Hu5Zhao-Jun Wei6School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaSchool of Biological Science and Engineering, North Minzu University, Yinchuan 750021, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaJiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212003, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaHydrogen sulfide (H<sub>2</sub>S) has been recognized for its beneficial influence on physiological alterations. The development (body weight) and economic characteristics (cocoon weight, cocoon shell ratio, and cocoon shell weight) of silkworms were increased after continuous 7.5 µM H<sub>2</sub>S treatment. In the present study, gene expression changes in the fat body of silkworms at the 5th instar larvae in response to the H<sub>2</sub>S were investigated through comparative transcriptome analysis. Moreover, the expression pattern of significant differentially expressed genes (DEGs) at the 5th instar larvae was confirmed by quantitative real-time PCR (qRT-PCR) after H<sub>2</sub>S exposure. A total of 1200 (DEGs) was identified, of which 977 DEGs were up-regulated and 223 DEGs were down-regulated. Most of the DEGs were involved in the transport pathway, cellular community, carbohydrate metabolism, and immune-associated signal transduction. The up regulated genes under H<sub>2</sub>S exposure were involved in endocytosis, glycolysis/gluconeogenesis, the citrate cycle (TCA cycle), and the synthesis of fibroin, while genes related to inflammation were down-regulated, indicating that H<sub>2</sub>S could promote energy metabolism, the transport pathway, silk synthesis, and inhibit inflammation in the silkworm. In addition, the expression levels of these genes were increased or decreased in a time-dependent manner during the 5th instar larvae. These results provided insight into the effects of H<sub>2</sub>S on silkworms at the transcriptional level and a substantial foundation for understanding H<sub>2</sub>S function.https://www.mdpi.com/2075-4450/12/12/1110silkwormhydrogen sulfidetranscriptomedifferentially expressed genesexpression pattern |
| spellingShingle | Rui Zhang Yu-Yao Cao Juan Du Kiran Thakur Shun-Ming Tang Fei Hu Zhao-Jun Wei Transcriptome Analysis Reveals the Gene Expression Changes in the Silkworm (<i>Bombyx mori</i>) in Response to Hydrogen Sulfide Exposure Insects silkworm hydrogen sulfide transcriptome differentially expressed genes expression pattern |
| title | Transcriptome Analysis Reveals the Gene Expression Changes in the Silkworm (<i>Bombyx mori</i>) in Response to Hydrogen Sulfide Exposure |
| title_full | Transcriptome Analysis Reveals the Gene Expression Changes in the Silkworm (<i>Bombyx mori</i>) in Response to Hydrogen Sulfide Exposure |
| title_fullStr | Transcriptome Analysis Reveals the Gene Expression Changes in the Silkworm (<i>Bombyx mori</i>) in Response to Hydrogen Sulfide Exposure |
| title_full_unstemmed | Transcriptome Analysis Reveals the Gene Expression Changes in the Silkworm (<i>Bombyx mori</i>) in Response to Hydrogen Sulfide Exposure |
| title_short | Transcriptome Analysis Reveals the Gene Expression Changes in the Silkworm (<i>Bombyx mori</i>) in Response to Hydrogen Sulfide Exposure |
| title_sort | transcriptome analysis reveals the gene expression changes in the silkworm i bombyx mori i in response to hydrogen sulfide exposure |
| topic | silkworm hydrogen sulfide transcriptome differentially expressed genes expression pattern |
| url | https://www.mdpi.com/2075-4450/12/12/1110 |
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