PAIP2 is a potential diagnostic and prognostic biomarker of breast cancer and is associated with immune infiltration
Breast cancer is the second highest incidence of cancer in the world. It is of great significance to find biomarkers to diagnose breast cancer and predict the prognosis of breast cancer patients. PAIP2 is a poly (A) -binding protein interacting protein that regulates the expression of VEGF. However,...
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| Format: | Article |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2022.1009056/full |
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| author | Chenyu Wang Chenyu Wang Xianglai Jiang Jiaojiao Qi Jiaojiao Qi Jiachao Xu Jiachao Xu Guangfei Yang Chengrong Mi |
| author_facet | Chenyu Wang Chenyu Wang Xianglai Jiang Jiaojiao Qi Jiaojiao Qi Jiachao Xu Jiachao Xu Guangfei Yang Chengrong Mi |
| author_sort | Chenyu Wang |
| collection | DOAJ |
| description | Breast cancer is the second highest incidence of cancer in the world. It is of great significance to find biomarkers to diagnose breast cancer and predict the prognosis of breast cancer patients. PAIP2 is a poly (A) -binding protein interacting protein that regulates the expression of VEGF. However, the possible role of PAIP2 in the progression of breast cancer is still unknown. RT-qRCR and Western blotting were used to verify the expression of PAIP2 in breast cancer cells and normal breast cells. The data of breast cancer samples were obtained in the TCGA database and the HPA database to analyze the expression of PAIP2 in breast cancer samples. Transwell experiment and CCK8 experiment confirmed the changes in the invasion and proliferation ability of PAIP2 after siRNA was down-regulated. Using bioinformatics technology to explore the prognostic value of PAIP2 and its possible biological function, and its effect on tumor immunity and immunotherapy. Studies have shown that PAIP2 has higher expression in breast cancer tissues and breast cancer cells. PAIP2 can promote the proliferation and invasion of breast cancer cells and has significantly high expression in higher tumor stages. The high expression of PAIP2 is associated with better OS in breast cancer patients and is negatively correlated with most chemotherapeutic drug sensitivity and IPS in cancer immunotherapy. Our study explored the potential value of PAIP2 as a biomarker for diagnosis and prognosis and may predict the efficacy of immunotherapy, providing reference for the follow-up study on the role of PAIP2 in breast cancer. |
| first_indexed | 2024-04-11T17:39:41Z |
| format | Article |
| id | doaj.art-402fae47856248d8ba70bf75c343566d |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-04-11T17:39:41Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-402fae47856248d8ba70bf75c343566d2022-12-22T04:11:31ZengFrontiers Media S.A.Frontiers in Genetics1664-80212022-11-011310.3389/fgene.2022.10090561009056PAIP2 is a potential diagnostic and prognostic biomarker of breast cancer and is associated with immune infiltrationChenyu Wang0Chenyu Wang1Xianglai Jiang2Jiaojiao Qi3Jiaojiao Qi4Jiachao Xu5Jiachao Xu6Guangfei Yang7Chengrong Mi8School of Clinical Medicine, Ningxia Medical University, Yinchuan, ChinaDepartment of Ultrasound, General Hospital of Ningxia Medical University, Yinchuan, ChinaSchool of Clinical Medicine, Ningxia Medical University, Yinchuan, ChinaSchool of Clinical Medicine, Ningxia Medical University, Yinchuan, ChinaDepartment of Ultrasound, General Hospital of Ningxia Medical University, Yinchuan, ChinaSchool of Clinical Medicine, Ningxia Medical University, Yinchuan, ChinaDepartment of Ultrasound, General Hospital of Ningxia Medical University, Yinchuan, ChinaDepartment of Ultrasound, General Hospital of Ningxia Medical University, Yinchuan, ChinaDepartment of Ultrasound, General Hospital of Ningxia Medical University, Yinchuan, ChinaBreast cancer is the second highest incidence of cancer in the world. It is of great significance to find biomarkers to diagnose breast cancer and predict the prognosis of breast cancer patients. PAIP2 is a poly (A) -binding protein interacting protein that regulates the expression of VEGF. However, the possible role of PAIP2 in the progression of breast cancer is still unknown. RT-qRCR and Western blotting were used to verify the expression of PAIP2 in breast cancer cells and normal breast cells. The data of breast cancer samples were obtained in the TCGA database and the HPA database to analyze the expression of PAIP2 in breast cancer samples. Transwell experiment and CCK8 experiment confirmed the changes in the invasion and proliferation ability of PAIP2 after siRNA was down-regulated. Using bioinformatics technology to explore the prognostic value of PAIP2 and its possible biological function, and its effect on tumor immunity and immunotherapy. Studies have shown that PAIP2 has higher expression in breast cancer tissues and breast cancer cells. PAIP2 can promote the proliferation and invasion of breast cancer cells and has significantly high expression in higher tumor stages. The high expression of PAIP2 is associated with better OS in breast cancer patients and is negatively correlated with most chemotherapeutic drug sensitivity and IPS in cancer immunotherapy. Our study explored the potential value of PAIP2 as a biomarker for diagnosis and prognosis and may predict the efficacy of immunotherapy, providing reference for the follow-up study on the role of PAIP2 in breast cancer.https://www.frontiersin.org/articles/10.3389/fgene.2022.1009056/fullPaip2TCGAbiomarkersbreast cancerimmune |
| spellingShingle | Chenyu Wang Chenyu Wang Xianglai Jiang Jiaojiao Qi Jiaojiao Qi Jiachao Xu Jiachao Xu Guangfei Yang Chengrong Mi PAIP2 is a potential diagnostic and prognostic biomarker of breast cancer and is associated with immune infiltration Frontiers in Genetics Paip2 TCGA biomarkers breast cancer immune |
| title | PAIP2 is a potential diagnostic and prognostic biomarker of breast cancer and is associated with immune infiltration |
| title_full | PAIP2 is a potential diagnostic and prognostic biomarker of breast cancer and is associated with immune infiltration |
| title_fullStr | PAIP2 is a potential diagnostic and prognostic biomarker of breast cancer and is associated with immune infiltration |
| title_full_unstemmed | PAIP2 is a potential diagnostic and prognostic biomarker of breast cancer and is associated with immune infiltration |
| title_short | PAIP2 is a potential diagnostic and prognostic biomarker of breast cancer and is associated with immune infiltration |
| title_sort | paip2 is a potential diagnostic and prognostic biomarker of breast cancer and is associated with immune infiltration |
| topic | Paip2 TCGA biomarkers breast cancer immune |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2022.1009056/full |
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