Digestive α-L-fucosidase activity in Rhodnius prolixus after blood feeding: effect of secretagogue and nutritional stimuli
Introduction:Rhodnius prolixus (Hemiptera: Reduviidae) is an important vector of Trypanosoma cruzi, the causative agent of Chagas Disease. This insect is a model for the study of insect physiology, especially concerning the digestion of blood. Among the enzymes produced in the midgut of R. prolixus...
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2023.1123414/full |
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| author | Maiara do Valle Faria Gama Yasmim do Nascimento Alexandre João Mario Pereira da Silva Daniele Pereira Castro Daniele Pereira Castro Fernando Ariel Genta Fernando Ariel Genta |
| author_facet | Maiara do Valle Faria Gama Yasmim do Nascimento Alexandre João Mario Pereira da Silva Daniele Pereira Castro Daniele Pereira Castro Fernando Ariel Genta Fernando Ariel Genta |
| author_sort | Maiara do Valle Faria Gama |
| collection | DOAJ |
| description | Introduction:Rhodnius prolixus (Hemiptera: Reduviidae) is an important vector of Trypanosoma cruzi, the causative agent of Chagas Disease. This insect is a model for the study of insect physiology, especially concerning the digestion of blood. Among the enzymes produced in the midgut of R. prolixus after blood feeding there is a α-L-fucosidase activity. There are very few studies on α-L-fucosidase of insects, and the role of R. prolixus α-L-fucosidase is still not clear. In this work, we tested if the mechanism for production of this enzyme is similar to the observed for proteases, a secretatogue mechanism that respond to the protein contents of the meal.Methods: We tested if specific proteins or sugars elicit this response, which may help to understand the nature of the physiological substrate for this enzyme.Results: In general, our results showed that the Anterior Midgut was the only midgut fraction that responds to the blood meal in terms of α-L-fucosidase production. Besides that, this response was not triggered by midgut distension or by ingestion of the blood cell fraction. Conversely, the enzyme was produced after feeding with the plasma fraction. However, the production of α-L-fucosidase was also triggered by different biochemical stimuli, as protein or fucoidan ingestion.Discussion: This suggested that the production of the enzyme in the anterior midgut was a general physiological response under control of different convergent signals. Besides that, the comparison between different treatments for artificial blood feeding showed that heparinated blood was the choice with minor side effects for the study of the midgut α-L-fucosidase, when compared to defibrinated or citrated blood. |
| first_indexed | 2024-03-12T23:03:35Z |
| format | Article |
| id | doaj.art-402fc9709d474e78a9c53cb32accc61c |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-03-12T23:03:35Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-402fc9709d474e78a9c53cb32accc61c2023-07-19T06:31:54ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2023-07-011410.3389/fphys.2023.11234141123414Digestive α-L-fucosidase activity in Rhodnius prolixus after blood feeding: effect of secretagogue and nutritional stimuliMaiara do Valle Faria Gama0Yasmim do Nascimento Alexandre1João Mario Pereira da Silva2Daniele Pereira Castro3Daniele Pereira Castro4Fernando Ariel Genta5Fernando Ariel Genta6Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, BrazilInstituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, BrazilInstituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, BrazilInstituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, BrazilInstituto Nacional de Ciência e Tecnologia em Entomologia Molecular, Rio de Janeiro, BrazilInstituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, BrazilInstituto Nacional de Ciência e Tecnologia em Entomologia Molecular, Rio de Janeiro, BrazilIntroduction:Rhodnius prolixus (Hemiptera: Reduviidae) is an important vector of Trypanosoma cruzi, the causative agent of Chagas Disease. This insect is a model for the study of insect physiology, especially concerning the digestion of blood. Among the enzymes produced in the midgut of R. prolixus after blood feeding there is a α-L-fucosidase activity. There are very few studies on α-L-fucosidase of insects, and the role of R. prolixus α-L-fucosidase is still not clear. In this work, we tested if the mechanism for production of this enzyme is similar to the observed for proteases, a secretatogue mechanism that respond to the protein contents of the meal.Methods: We tested if specific proteins or sugars elicit this response, which may help to understand the nature of the physiological substrate for this enzyme.Results: In general, our results showed that the Anterior Midgut was the only midgut fraction that responds to the blood meal in terms of α-L-fucosidase production. Besides that, this response was not triggered by midgut distension or by ingestion of the blood cell fraction. Conversely, the enzyme was produced after feeding with the plasma fraction. However, the production of α-L-fucosidase was also triggered by different biochemical stimuli, as protein or fucoidan ingestion.Discussion: This suggested that the production of the enzyme in the anterior midgut was a general physiological response under control of different convergent signals. Besides that, the comparison between different treatments for artificial blood feeding showed that heparinated blood was the choice with minor side effects for the study of the midgut α-L-fucosidase, when compared to defibrinated or citrated blood.https://www.frontiersin.org/articles/10.3389/fphys.2023.1123414/fullα-fucosidaseRhodnius prolixusblood digestioncarbohydrasevector physiology |
| spellingShingle | Maiara do Valle Faria Gama Yasmim do Nascimento Alexandre João Mario Pereira da Silva Daniele Pereira Castro Daniele Pereira Castro Fernando Ariel Genta Fernando Ariel Genta Digestive α-L-fucosidase activity in Rhodnius prolixus after blood feeding: effect of secretagogue and nutritional stimuli Frontiers in Physiology α-fucosidase Rhodnius prolixus blood digestion carbohydrase vector physiology |
| title | Digestive α-L-fucosidase activity in Rhodnius prolixus after blood feeding: effect of secretagogue and nutritional stimuli |
| title_full | Digestive α-L-fucosidase activity in Rhodnius prolixus after blood feeding: effect of secretagogue and nutritional stimuli |
| title_fullStr | Digestive α-L-fucosidase activity in Rhodnius prolixus after blood feeding: effect of secretagogue and nutritional stimuli |
| title_full_unstemmed | Digestive α-L-fucosidase activity in Rhodnius prolixus after blood feeding: effect of secretagogue and nutritional stimuli |
| title_short | Digestive α-L-fucosidase activity in Rhodnius prolixus after blood feeding: effect of secretagogue and nutritional stimuli |
| title_sort | digestive α l fucosidase activity in rhodnius prolixus after blood feeding effect of secretagogue and nutritional stimuli |
| topic | α-fucosidase Rhodnius prolixus blood digestion carbohydrase vector physiology |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2023.1123414/full |
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