Genome-wide analysis of factors affecting transcription elongation and DNA repair: a new role for PAF and Ccr4-not in transcription-coupled repair.
RNA polymerases frequently deal with a number of obstacles during transcription elongation that need to be removed for transcription resumption. One important type of hindrance consists of DNA lesions, which are removed by transcription-coupled repair (TC-NER), a specific sub-pathway of nucleotide e...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2009-02-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC2629578?pdf=render |
| _version_ | 1818850185245097984 |
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| author | Hélène Gaillard Cristina Tous Javier Botet Cristina González-Aguilera Maria José Quintero Laia Viladevall María L García-Rubio Alfonso Rodríguez-Gil Antonio Marín Joaquín Ariño José Luis Revuelta Sebastián Chávez Andrés Aguilera |
| author_facet | Hélène Gaillard Cristina Tous Javier Botet Cristina González-Aguilera Maria José Quintero Laia Viladevall María L García-Rubio Alfonso Rodríguez-Gil Antonio Marín Joaquín Ariño José Luis Revuelta Sebastián Chávez Andrés Aguilera |
| author_sort | Hélène Gaillard |
| collection | DOAJ |
| description | RNA polymerases frequently deal with a number of obstacles during transcription elongation that need to be removed for transcription resumption. One important type of hindrance consists of DNA lesions, which are removed by transcription-coupled repair (TC-NER), a specific sub-pathway of nucleotide excision repair. To improve our knowledge of transcription elongation and its coupling to TC-NER, we used the yeast library of non-essential knock-out mutations to screen for genes conferring resistance to the transcription-elongation inhibitor mycophenolic acid and the DNA-damaging agent 4-nitroquinoline-N-oxide. Our data provide evidence that subunits of the SAGA and Ccr4-Not complexes, Mediator, Bre1, Bur2, and Fun12 affect transcription elongation to different extents. Given the dependency of TC-NER on RNA Polymerase II transcription and the fact that the few proteins known to be involved in TC-NER are related to transcription, we performed an in-depth TC-NER analysis of a selection of mutants. We found that mutants of the PAF and Ccr4-Not complexes are impaired in TC-NER. This study provides evidence that PAF and Ccr4-Not are required for efficient TC-NER in yeast, unraveling a novel function for these transcription complexes and opening new perspectives for the understanding of TC-NER and its functional interconnection with transcription elongation. |
| first_indexed | 2024-12-19T06:45:07Z |
| format | Article |
| id | doaj.art-40303625978e46cfb6a75602b0e5a290 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-19T06:45:07Z |
| publishDate | 2009-02-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-40303625978e46cfb6a75602b0e5a2902022-12-21T20:31:54ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042009-02-0152e100036410.1371/journal.pgen.1000364Genome-wide analysis of factors affecting transcription elongation and DNA repair: a new role for PAF and Ccr4-not in transcription-coupled repair.Hélène GaillardCristina TousJavier BotetCristina González-AguileraMaria José QuinteroLaia ViladevallMaría L García-RubioAlfonso Rodríguez-GilAntonio MarínJoaquín AriñoJosé Luis RevueltaSebastián ChávezAndrés AguileraRNA polymerases frequently deal with a number of obstacles during transcription elongation that need to be removed for transcription resumption. One important type of hindrance consists of DNA lesions, which are removed by transcription-coupled repair (TC-NER), a specific sub-pathway of nucleotide excision repair. To improve our knowledge of transcription elongation and its coupling to TC-NER, we used the yeast library of non-essential knock-out mutations to screen for genes conferring resistance to the transcription-elongation inhibitor mycophenolic acid and the DNA-damaging agent 4-nitroquinoline-N-oxide. Our data provide evidence that subunits of the SAGA and Ccr4-Not complexes, Mediator, Bre1, Bur2, and Fun12 affect transcription elongation to different extents. Given the dependency of TC-NER on RNA Polymerase II transcription and the fact that the few proteins known to be involved in TC-NER are related to transcription, we performed an in-depth TC-NER analysis of a selection of mutants. We found that mutants of the PAF and Ccr4-Not complexes are impaired in TC-NER. This study provides evidence that PAF and Ccr4-Not are required for efficient TC-NER in yeast, unraveling a novel function for these transcription complexes and opening new perspectives for the understanding of TC-NER and its functional interconnection with transcription elongation.http://europepmc.org/articles/PMC2629578?pdf=render |
| spellingShingle | Hélène Gaillard Cristina Tous Javier Botet Cristina González-Aguilera Maria José Quintero Laia Viladevall María L García-Rubio Alfonso Rodríguez-Gil Antonio Marín Joaquín Ariño José Luis Revuelta Sebastián Chávez Andrés Aguilera Genome-wide analysis of factors affecting transcription elongation and DNA repair: a new role for PAF and Ccr4-not in transcription-coupled repair. PLoS Genetics |
| title | Genome-wide analysis of factors affecting transcription elongation and DNA repair: a new role for PAF and Ccr4-not in transcription-coupled repair. |
| title_full | Genome-wide analysis of factors affecting transcription elongation and DNA repair: a new role for PAF and Ccr4-not in transcription-coupled repair. |
| title_fullStr | Genome-wide analysis of factors affecting transcription elongation and DNA repair: a new role for PAF and Ccr4-not in transcription-coupled repair. |
| title_full_unstemmed | Genome-wide analysis of factors affecting transcription elongation and DNA repair: a new role for PAF and Ccr4-not in transcription-coupled repair. |
| title_short | Genome-wide analysis of factors affecting transcription elongation and DNA repair: a new role for PAF and Ccr4-not in transcription-coupled repair. |
| title_sort | genome wide analysis of factors affecting transcription elongation and dna repair a new role for paf and ccr4 not in transcription coupled repair |
| url | http://europepmc.org/articles/PMC2629578?pdf=render |
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