Candida biome of severe early childhood caries (S-ECC) and its cariogenic virulence traits
The protected niche of deep-caries lesions is a distinctive ecosystem. We assessed the Candida biome and its cariogenic traits from dentin samples of 50 children with severe-early childhood caries (S-ECC). Asymptomatic, primary molars belonging to International Caries Detection and Assessment-ICDAS...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Journal of Oral Microbiology |
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| Online Access: | http://dx.doi.org/10.1080/20002297.2020.1724484 |
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| author | Kausar Sadia Fakhruddin Lakshman Perera Samaranayake Hiroshi Egusa Hien Chi Ngo Chamila Panduwawala Thenmozhi Venkatachalam Allagappan Kumarappan Siripen Pesee |
| author_facet | Kausar Sadia Fakhruddin Lakshman Perera Samaranayake Hiroshi Egusa Hien Chi Ngo Chamila Panduwawala Thenmozhi Venkatachalam Allagappan Kumarappan Siripen Pesee |
| author_sort | Kausar Sadia Fakhruddin |
| collection | DOAJ |
| description | The protected niche of deep-caries lesions is a distinctive ecosystem. We assessed the Candida biome and its cariogenic traits from dentin samples of 50 children with severe-early childhood caries (S-ECC). Asymptomatic, primary molars belonging to International Caries Detection and Assessment-ICDAS caries-code 5 and 6 were analyzed, and C. albicans (10-isolates), C. tropicalis (10), C. krusei (10), and C. glabrata (5) isolated from the lesions were then evaluated for their biofilm formation, acidogenicity, and the production of secreted hydrolases: hemolysins, phospholipase, proteinase and DNase. Candida were isolated from 14/43 ICDAS-5 lesions (32.5%) and 44/57 ICDAS-6 lesions (77.2%). Compared to, ICDAS-5, a significantly higher frequency of multi-species infestation was observed in ICDAS-6 lesions (p=0.001). All four candidal species (above) showed prolific biofilm growth, and an equal potency for tooth demineralization. A significant interspecies difference in the mean phospholipase, as well as proteinase activity was noted (p < 0.05), with C. albicans being the predominant hydrolase producer. Further, a positive correlation between phospholipase and proteinase activity of Candida-isolates was noted (r = 0.818, p < 0.001). Our data suggest that candidal mycobiota with their potent cariogenic traits may significantly contribute to the development and progression of S-ECC. |
| first_indexed | 2024-12-14T22:59:56Z |
| format | Article |
| id | doaj.art-4030d799c0f247878a9379154e57a5d0 |
| institution | Directory Open Access Journal |
| issn | 2000-2297 |
| language | English |
| last_indexed | 2024-12-14T22:59:56Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj.art-4030d799c0f247878a9379154e57a5d02022-12-21T22:44:29ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972020-01-0112110.1080/20002297.2020.17244841724484Candida biome of severe early childhood caries (S-ECC) and its cariogenic virulence traitsKausar Sadia Fakhruddin0Lakshman Perera Samaranayake1Hiroshi Egusa2Hien Chi Ngo3Chamila Panduwawala4Thenmozhi Venkatachalam5Allagappan Kumarappan6Siripen Pesee7University of SharjahUniversity of SharjahTohoku University Graduate School of DentistryUniversity of SharjahUniversity of SharjahUniversity of SharjahUniversity of SharjahThammasat UniversityThe protected niche of deep-caries lesions is a distinctive ecosystem. We assessed the Candida biome and its cariogenic traits from dentin samples of 50 children with severe-early childhood caries (S-ECC). Asymptomatic, primary molars belonging to International Caries Detection and Assessment-ICDAS caries-code 5 and 6 were analyzed, and C. albicans (10-isolates), C. tropicalis (10), C. krusei (10), and C. glabrata (5) isolated from the lesions were then evaluated for their biofilm formation, acidogenicity, and the production of secreted hydrolases: hemolysins, phospholipase, proteinase and DNase. Candida were isolated from 14/43 ICDAS-5 lesions (32.5%) and 44/57 ICDAS-6 lesions (77.2%). Compared to, ICDAS-5, a significantly higher frequency of multi-species infestation was observed in ICDAS-6 lesions (p=0.001). All four candidal species (above) showed prolific biofilm growth, and an equal potency for tooth demineralization. A significant interspecies difference in the mean phospholipase, as well as proteinase activity was noted (p < 0.05), with C. albicans being the predominant hydrolase producer. Further, a positive correlation between phospholipase and proteinase activity of Candida-isolates was noted (r = 0.818, p < 0.001). Our data suggest that candidal mycobiota with their potent cariogenic traits may significantly contribute to the development and progression of S-ECC.http://dx.doi.org/10.1080/20002297.2020.1724484candida speciessevere early childhood caries (s-ecc)dentin carieshydrolasesbiofilmcalcium-releaseacidogenicityhaemolysinphopholipaseprotease |
| spellingShingle | Kausar Sadia Fakhruddin Lakshman Perera Samaranayake Hiroshi Egusa Hien Chi Ngo Chamila Panduwawala Thenmozhi Venkatachalam Allagappan Kumarappan Siripen Pesee Candida biome of severe early childhood caries (S-ECC) and its cariogenic virulence traits Journal of Oral Microbiology candida species severe early childhood caries (s-ecc) dentin caries hydrolases biofilm calcium-release acidogenicity haemolysin phopholipase protease |
| title | Candida biome of severe early childhood caries (S-ECC) and its cariogenic virulence traits |
| title_full | Candida biome of severe early childhood caries (S-ECC) and its cariogenic virulence traits |
| title_fullStr | Candida biome of severe early childhood caries (S-ECC) and its cariogenic virulence traits |
| title_full_unstemmed | Candida biome of severe early childhood caries (S-ECC) and its cariogenic virulence traits |
| title_short | Candida biome of severe early childhood caries (S-ECC) and its cariogenic virulence traits |
| title_sort | candida biome of severe early childhood caries s ecc and its cariogenic virulence traits |
| topic | candida species severe early childhood caries (s-ecc) dentin caries hydrolases biofilm calcium-release acidogenicity haemolysin phopholipase protease |
| url | http://dx.doi.org/10.1080/20002297.2020.1724484 |
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