Effect of green alga Planktochlorella nurekis on selected bacteria revealed antibacterial activity in vitro
The green alga Planktochlorella nurekis (Chlorellaceae, Chlorophyta) is considered a producer of antibacterial mixture of long-chain fatty acids, which has possibly similar composition and mode of action as chlorellin produced by another green alga, Chlorella vulgaris. Although the antibacterial pro...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2015-10-01
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| Series: | Czech Journal of Animal Science |
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| Online Access: | https://cjas.agriculturejournals.cz/artkey/cjs-201510-0001_effect-of-green-alga-planktochlorella-nurekis-on-selected-bacteria-revealed-antibacterial-activity-in-vitro.php |
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| author | L. Čermák Š. Pražáková M. Marounek M. Skřivan E. Skřivanová |
| author_facet | L. Čermák Š. Pražáková M. Marounek M. Skřivan E. Skřivanová |
| author_sort | L. Čermák |
| collection | DOAJ |
| description | The green alga Planktochlorella nurekis (Chlorellaceae, Chlorophyta) is considered a producer of antibacterial mixture of long-chain fatty acids, which has possibly similar composition and mode of action as chlorellin produced by another green alga, Chlorella vulgaris. Although the antibacterial properties of C. vulgaris have been reported, the interactions of P. nurekis with bacteria have not been determined yet. The aim of this study was to elucidate the effect of P. nurekis water suspension on growth of selected gastrointestinal bacteria in vitro so that it could be used as a suitable feed supplement in animal farming. Unknown bacterial populations occurring in the algal suspension were identified using 16S rRNA sequencing assay. Selected strains were cultivated with lyophilized P. nurekis and the antibacterial effect was monitored. The composition of fatty acids and heat sensitivity of antibacterial substances were also examined. Sequencing analysis of 71 bacterial 16S rRNA genes in xenic algal suspension identified common environmental microbiota, one strain belonging to the class Alphaproteobacteria, 17 to Betaproteobacteria, 44 to Gammaproteobacteria (dominated by Pseudomonas putida strains), and nine to Sphingobacteria. The antimicrobial activity of P. nurekis suspension was tested at a concentration range of 0.75-6 mg/ml. The highest inhibitory effect was observed on bifidobacteria. Statistically significant reductions in bacterial counts were also observed for Escherichia coli, Salmonella enterica var. Enteritidis, S. enterica var. Infantis, Campylobacter jejuni, and Arcobacter butzleri. The growth of Lactobacillus johnsonii was significantly stimulated. The relative proportions of C14-C22 fatty acids in P. nurekis were found as follows: saturated 54.28%, monounsaturated 30.40%, and polyunsaturated 7.16%. The antibacterial compounds present in P. nurekis suspension exhibited thermostability. The results indicate that P. nurekis can inhibit some pathogenic gastrointestinal bacteria and seems to be a promising essential nutrients source in animal nutrition. |
| first_indexed | 2024-04-10T08:25:58Z |
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| id | doaj.art-40324decb7d44781927a6dda5251c665 |
| institution | Directory Open Access Journal |
| issn | 1212-1819 1805-9309 |
| language | English |
| last_indexed | 2024-04-10T08:25:58Z |
| publishDate | 2015-10-01 |
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| series | Czech Journal of Animal Science |
| spelling | doaj.art-40324decb7d44781927a6dda5251c6652023-02-23T03:33:19ZengCzech Academy of Agricultural SciencesCzech Journal of Animal Science1212-18191805-93092015-10-01601042743510.17221/8522-CJAScjs-201510-0001Effect of green alga Planktochlorella nurekis on selected bacteria revealed antibacterial activity in vitroL. Čermák0Š. Pražáková1M. Marounek2M. Skřivan3E. Skřivanová4Institute of Animal Science, Prague-Uhříněves, Czech RepublicInstitute of Animal Science, Prague-Uhříněves, Czech RepublicInstitute of Animal Science, Prague-Uhříněves, Czech RepublicInstitute of Animal Science, Prague-Uhříněves, Czech RepublicInstitute of Animal Science, Prague-Uhříněves, Czech RepublicThe green alga Planktochlorella nurekis (Chlorellaceae, Chlorophyta) is considered a producer of antibacterial mixture of long-chain fatty acids, which has possibly similar composition and mode of action as chlorellin produced by another green alga, Chlorella vulgaris. Although the antibacterial properties of C. vulgaris have been reported, the interactions of P. nurekis with bacteria have not been determined yet. The aim of this study was to elucidate the effect of P. nurekis water suspension on growth of selected gastrointestinal bacteria in vitro so that it could be used as a suitable feed supplement in animal farming. Unknown bacterial populations occurring in the algal suspension were identified using 16S rRNA sequencing assay. Selected strains were cultivated with lyophilized P. nurekis and the antibacterial effect was monitored. The composition of fatty acids and heat sensitivity of antibacterial substances were also examined. Sequencing analysis of 71 bacterial 16S rRNA genes in xenic algal suspension identified common environmental microbiota, one strain belonging to the class Alphaproteobacteria, 17 to Betaproteobacteria, 44 to Gammaproteobacteria (dominated by Pseudomonas putida strains), and nine to Sphingobacteria. The antimicrobial activity of P. nurekis suspension was tested at a concentration range of 0.75-6 mg/ml. The highest inhibitory effect was observed on bifidobacteria. Statistically significant reductions in bacterial counts were also observed for Escherichia coli, Salmonella enterica var. Enteritidis, S. enterica var. Infantis, Campylobacter jejuni, and Arcobacter butzleri. The growth of Lactobacillus johnsonii was significantly stimulated. The relative proportions of C14-C22 fatty acids in P. nurekis were found as follows: saturated 54.28%, monounsaturated 30.40%, and polyunsaturated 7.16%. The antibacterial compounds present in P. nurekis suspension exhibited thermostability. The results indicate that P. nurekis can inhibit some pathogenic gastrointestinal bacteria and seems to be a promising essential nutrients source in animal nutrition.https://cjas.agriculturejournals.cz/artkey/cjs-201510-0001_effect-of-green-alga-planktochlorella-nurekis-on-selected-bacteria-revealed-antibacterial-activity-in-vitro.phpchlorellinfatty acidsgastrointestinal bacteriainteractionmicroalgaethermostability |
| spellingShingle | L. Čermák Š. Pražáková M. Marounek M. Skřivan E. Skřivanová Effect of green alga Planktochlorella nurekis on selected bacteria revealed antibacterial activity in vitro Czech Journal of Animal Science chlorellin fatty acids gastrointestinal bacteria interaction microalgae thermostability |
| title | Effect of green alga Planktochlorella nurekis on selected bacteria revealed antibacterial activity in vitro |
| title_full | Effect of green alga Planktochlorella nurekis on selected bacteria revealed antibacterial activity in vitro |
| title_fullStr | Effect of green alga Planktochlorella nurekis on selected bacteria revealed antibacterial activity in vitro |
| title_full_unstemmed | Effect of green alga Planktochlorella nurekis on selected bacteria revealed antibacterial activity in vitro |
| title_short | Effect of green alga Planktochlorella nurekis on selected bacteria revealed antibacterial activity in vitro |
| title_sort | effect of green alga planktochlorella nurekis on selected bacteria revealed antibacterial activity in vitro |
| topic | chlorellin fatty acids gastrointestinal bacteria interaction microalgae thermostability |
| url | https://cjas.agriculturejournals.cz/artkey/cjs-201510-0001_effect-of-green-alga-planktochlorella-nurekis-on-selected-bacteria-revealed-antibacterial-activity-in-vitro.php |
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