Antibody-Conjugated Magnetic Beads for Sperm Sexing Using a Multi-Wall Carbon Nanotube Microfluidic Device
This study proposes a microfluidic device used for X-/Y-sperm separation based on monoclonal antibody-conjugated magnetic beads, which become positively charged in the flow system. Y-sperms were selectively captured via a monoclonal antibody and transferred onto the microfluidic device and were disc...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/13/3/426 |
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| author | Chalinee Phiphattanaphiphop Komgrit Leksakul Thananut Wanta Trisadee Khamlor Rungrueang Phattanakun |
| author_facet | Chalinee Phiphattanaphiphop Komgrit Leksakul Thananut Wanta Trisadee Khamlor Rungrueang Phattanakun |
| author_sort | Chalinee Phiphattanaphiphop |
| collection | DOAJ |
| description | This study proposes a microfluidic device used for X-/Y-sperm separation based on monoclonal antibody-conjugated magnetic beads, which become positively charged in the flow system. Y-sperms were selectively captured via a monoclonal antibody and transferred onto the microfluidic device and were discarded, so that X-sperms can be isolated and commercially exploited for fertilization demands of female cattle in dairy industry. Therefore, the research team used monoclonal antibody-conjugated magnetic beads to increase the force that causes the Y-sperm to be pulled out of the system, leaving only the X-sperm for further use. The experimental design was divided into the following: Model 1, the microfluid system for sorting positive magnetic beads, which yielded 100% separation; Model 2, the sorting of monoclonal antibody-conjugated magnetic beads in the fluid system, yielding 98.84% microcirculation; Model 3, the sorting of monoclonal antibody-conjugated magnetic beads with sperm in the microfluid system, yielding 80.12% microcirculation. Moreover, the fabrication microfluidic system had thin film electrodes created via UV lithography and MWCNTs electrode structure capable of erecting an electrode wall 1500 µm above the floor with a flow channel width of only 100 µm. The system was tested using a constant flow rate of 2 µL/min and X-/Y-sperm were separated using carbon nanotube electrodes at 2.5 V. The structure created with the use of vertical electrodes and monoclonal antibody-conjugated magnetic beads technique produced a higher effective rejection effect and was able to remove a large number of unwanted sperm from the system with 80.12% efficiency. |
| first_indexed | 2024-03-09T13:17:17Z |
| format | Article |
| id | doaj.art-4032d2141b7a40a5878b83f4fb6c5a60 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-09T13:17:17Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-4032d2141b7a40a5878b83f4fb6c5a602023-11-30T21:34:00ZengMDPI AGMicromachines2072-666X2022-03-0113342610.3390/mi13030426Antibody-Conjugated Magnetic Beads for Sperm Sexing Using a Multi-Wall Carbon Nanotube Microfluidic DeviceChalinee Phiphattanaphiphop0Komgrit Leksakul1Thananut Wanta2Trisadee Khamlor3Rungrueang Phattanakun4Industrial Engineering Department, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, ThailandIndustrial Engineering Department, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, ThailandIndustrial Engineering Department, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, ThailandAnimal and Aquatic Science Department, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, ThailandSynchrotron Light Research Institute, Nakhon Ratchasima 30000, ThailandThis study proposes a microfluidic device used for X-/Y-sperm separation based on monoclonal antibody-conjugated magnetic beads, which become positively charged in the flow system. Y-sperms were selectively captured via a monoclonal antibody and transferred onto the microfluidic device and were discarded, so that X-sperms can be isolated and commercially exploited for fertilization demands of female cattle in dairy industry. Therefore, the research team used monoclonal antibody-conjugated magnetic beads to increase the force that causes the Y-sperm to be pulled out of the system, leaving only the X-sperm for further use. The experimental design was divided into the following: Model 1, the microfluid system for sorting positive magnetic beads, which yielded 100% separation; Model 2, the sorting of monoclonal antibody-conjugated magnetic beads in the fluid system, yielding 98.84% microcirculation; Model 3, the sorting of monoclonal antibody-conjugated magnetic beads with sperm in the microfluid system, yielding 80.12% microcirculation. Moreover, the fabrication microfluidic system had thin film electrodes created via UV lithography and MWCNTs electrode structure capable of erecting an electrode wall 1500 µm above the floor with a flow channel width of only 100 µm. The system was tested using a constant flow rate of 2 µL/min and X-/Y-sperm were separated using carbon nanotube electrodes at 2.5 V. The structure created with the use of vertical electrodes and monoclonal antibody-conjugated magnetic beads technique produced a higher effective rejection effect and was able to remove a large number of unwanted sperm from the system with 80.12% efficiency.https://www.mdpi.com/2072-666X/13/3/426monoclonal antibody conjugatedsperm sexingmulti-wall carbon nanotubesmagnetic beadsmicrofluidic |
| spellingShingle | Chalinee Phiphattanaphiphop Komgrit Leksakul Thananut Wanta Trisadee Khamlor Rungrueang Phattanakun Antibody-Conjugated Magnetic Beads for Sperm Sexing Using a Multi-Wall Carbon Nanotube Microfluidic Device Micromachines monoclonal antibody conjugated sperm sexing multi-wall carbon nanotubes magnetic beads microfluidic |
| title | Antibody-Conjugated Magnetic Beads for Sperm Sexing Using a Multi-Wall Carbon Nanotube Microfluidic Device |
| title_full | Antibody-Conjugated Magnetic Beads for Sperm Sexing Using a Multi-Wall Carbon Nanotube Microfluidic Device |
| title_fullStr | Antibody-Conjugated Magnetic Beads for Sperm Sexing Using a Multi-Wall Carbon Nanotube Microfluidic Device |
| title_full_unstemmed | Antibody-Conjugated Magnetic Beads for Sperm Sexing Using a Multi-Wall Carbon Nanotube Microfluidic Device |
| title_short | Antibody-Conjugated Magnetic Beads for Sperm Sexing Using a Multi-Wall Carbon Nanotube Microfluidic Device |
| title_sort | antibody conjugated magnetic beads for sperm sexing using a multi wall carbon nanotube microfluidic device |
| topic | monoclonal antibody conjugated sperm sexing multi-wall carbon nanotubes magnetic beads microfluidic |
| url | https://www.mdpi.com/2072-666X/13/3/426 |
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