Detection and Phylogenetic Characterization of a Novel Herpesvirus in Sooty Terns Onychoprion fuscatus
Since 2005, we have recorded annual episodes of alphaherpesvirus outbreaks in chicks of magnificent frigatebird Fregata magnificens on the Ile du Grand Connétable Nature Reserve in French Guiana. In 2009, we found sooty terns, Onychoprion fuscatus, that live sympatrically with frigatebirds, with vis...
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Frontiers Media S.A.
2020-08-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fvets.2020.00567/full |
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| author | Manrico Sebastiano Daniele Canestrelli Roberta Bisconti Anne Lavergne Kévin Pineau Olivier Chastel Vincent Lacoste Vincent Lacoste David Costantini |
| author_facet | Manrico Sebastiano Daniele Canestrelli Roberta Bisconti Anne Lavergne Kévin Pineau Olivier Chastel Vincent Lacoste Vincent Lacoste David Costantini |
| author_sort | Manrico Sebastiano |
| collection | DOAJ |
| description | Since 2005, we have recorded annual episodes of alphaherpesvirus outbreaks in chicks of magnificent frigatebird Fregata magnificens on the Ile du Grand Connétable Nature Reserve in French Guiana. In 2009, we found sooty terns, Onychoprion fuscatus, that live sympatrically with frigatebirds, with visible clinical signs of a potential viral infection. To determine if the symptoms observed in sooty terns could be associated with an alphaherpesvirus previously identified in frigatebirds, we carried out molecular screening of samples collected from seven individuals. We identified and characterized a novel viral sequence from five birds. BLAST searches, pairwise nucleotide, and amino acid sequence comparisons, as well as phylogenetic analyses confirmed that the sequence belonged to the Herpesviridae family, of the Alphaherpesvirinae subfamily. We observed that it clustered with strains isolated from Podargidae (Caprimulgiformes), Columbiformes, and Falconiformes, but was distinct from the frigatebird herpesvirus. We have tentatively named it Onychoprion fuscatus alphaherpesvirus 1, (OfusAHV1). These two sequences, although found syntopic on the Ile du Grand Connétable, belong to two distinct alphaherpesvirus strains. Thus, the clinical symptoms showed by sooty terns do not likely result from a cross-species transmission event. Future work is needed to better characterize the virus and to investigate herpesvirus prevalence in healthy, free-ranging sooty terns, and to assess the impact of the virus on population viability. |
| first_indexed | 2024-12-10T15:17:36Z |
| format | Article |
| id | doaj.art-4036b63b25e941acb54c8fb6921edcec |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-10T15:17:36Z |
| publishDate | 2020-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-4036b63b25e941acb54c8fb6921edcec2022-12-22T01:43:45ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692020-08-01710.3389/fvets.2020.00567563309Detection and Phylogenetic Characterization of a Novel Herpesvirus in Sooty Terns Onychoprion fuscatusManrico Sebastiano0Daniele Canestrelli1Roberta Bisconti2Anne Lavergne3Kévin Pineau4Olivier Chastel5Vincent Lacoste6Vincent Lacoste7David Costantini8Centre d'Etudes Biologiques de Chizé (CEBC), UMR 7372 CNRS-Univ, La Rochelle, FranceDepartment of Ecological and Biological Science, Tuscia University, Viterbo, ItalyDepartment of Ecological and Biological Science, Tuscia University, Viterbo, ItalyLaboratoire des Interactions Virus-Hôtes, Institut Pasteur de la Guyane, Cayenne, FranceGroupe d'Etude et de Protection des Oiseaux en Guyane (GEPOG), Rémire-Montjoly, FranceCentre d'Etudes Biologiques de Chizé (CEBC), UMR 7372 CNRS-Univ, La Rochelle, FranceLaboratoire des Interactions Virus-Hôtes, Institut Pasteur de la Guyane, Cayenne, FranceUnité de Biologie des Infections Virales Emergentes, Centre International de Recherche en Infectiologie, Institut Pasteur, Lyon, FranceUnité Physiologie moléculaire et adaptation (PhyMA), Muséum National d'Histoire Naturelle, CNRS, CP32, Paris, FranceSince 2005, we have recorded annual episodes of alphaherpesvirus outbreaks in chicks of magnificent frigatebird Fregata magnificens on the Ile du Grand Connétable Nature Reserve in French Guiana. In 2009, we found sooty terns, Onychoprion fuscatus, that live sympatrically with frigatebirds, with visible clinical signs of a potential viral infection. To determine if the symptoms observed in sooty terns could be associated with an alphaherpesvirus previously identified in frigatebirds, we carried out molecular screening of samples collected from seven individuals. We identified and characterized a novel viral sequence from five birds. BLAST searches, pairwise nucleotide, and amino acid sequence comparisons, as well as phylogenetic analyses confirmed that the sequence belonged to the Herpesviridae family, of the Alphaherpesvirinae subfamily. We observed that it clustered with strains isolated from Podargidae (Caprimulgiformes), Columbiformes, and Falconiformes, but was distinct from the frigatebird herpesvirus. We have tentatively named it Onychoprion fuscatus alphaherpesvirus 1, (OfusAHV1). These two sequences, although found syntopic on the Ile du Grand Connétable, belong to two distinct alphaherpesvirus strains. Thus, the clinical symptoms showed by sooty terns do not likely result from a cross-species transmission event. Future work is needed to better characterize the virus and to investigate herpesvirus prevalence in healthy, free-ranging sooty terns, and to assess the impact of the virus on population viability.https://www.frontiersin.org/article/10.3389/fvets.2020.00567/fullalphaherpesvirusFrench GuianaOnychoprion fuscatusseabirdsdisease |
| spellingShingle | Manrico Sebastiano Daniele Canestrelli Roberta Bisconti Anne Lavergne Kévin Pineau Olivier Chastel Vincent Lacoste Vincent Lacoste David Costantini Detection and Phylogenetic Characterization of a Novel Herpesvirus in Sooty Terns Onychoprion fuscatus Frontiers in Veterinary Science alphaherpesvirus French Guiana Onychoprion fuscatus seabirds disease |
| title | Detection and Phylogenetic Characterization of a Novel Herpesvirus in Sooty Terns Onychoprion fuscatus |
| title_full | Detection and Phylogenetic Characterization of a Novel Herpesvirus in Sooty Terns Onychoprion fuscatus |
| title_fullStr | Detection and Phylogenetic Characterization of a Novel Herpesvirus in Sooty Terns Onychoprion fuscatus |
| title_full_unstemmed | Detection and Phylogenetic Characterization of a Novel Herpesvirus in Sooty Terns Onychoprion fuscatus |
| title_short | Detection and Phylogenetic Characterization of a Novel Herpesvirus in Sooty Terns Onychoprion fuscatus |
| title_sort | detection and phylogenetic characterization of a novel herpesvirus in sooty terns onychoprion fuscatus |
| topic | alphaherpesvirus French Guiana Onychoprion fuscatus seabirds disease |
| url | https://www.frontiersin.org/article/10.3389/fvets.2020.00567/full |
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