Effect of docosahexaenoic acid as a chemopreventive agent on experimentally induced hamster buccal pouch carcinogenesis
Purpose: The current study was directed to investigate the effectiveness of docosahexaenoic acid (DHA) as a chemopreventive agent on experimentally induced hamster buccal pouch (HBP) carcinogenesis. Material and methods: In this study we used 40 Syrian male hamsters, five weeks old, were divided int...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-01-01
|
| Series: | Cancer Treatment and Research Communications |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S246829422200048X |
| _version_ | 1811255170364866560 |
|---|---|
| author | Emad Mohamed Alqalshy Amr Mohamed Ibrahim Ahmed Abdel-Shakour Abdel-Hafiz Kamal Abd El-Rahman Kamal Magdy Alabasiry Alazzazi Mohamed Refaat Omar Amr Saad Abdel-Wahab Saher Sayed Mohammed |
| author_facet | Emad Mohamed Alqalshy Amr Mohamed Ibrahim Ahmed Abdel-Shakour Abdel-Hafiz Kamal Abd El-Rahman Kamal Magdy Alabasiry Alazzazi Mohamed Refaat Omar Amr Saad Abdel-Wahab Saher Sayed Mohammed |
| author_sort | Emad Mohamed Alqalshy |
| collection | DOAJ |
| description | Purpose: The current study was directed to investigate the effectiveness of docosahexaenoic acid (DHA) as a chemopreventive agent on experimentally induced hamster buccal pouch (HBP) carcinogenesis. Material and methods: In this study we used 40 Syrian male hamsters, five weeks old, were divided into 4 groups (GI, GII, GIII, and GIV) of 10 animals in each as follows, GI: Topical application of liquid paraffin alone (thrice a week for 14 weeks), GII: Topical application of 7, 12 dimethyl benz[a]anthracene (DMBA) alone (0.5% in liquid paraffin, thrice a week for 14 weeks), GIII: Topical application of DMBA (0.5% in liquid paraffin, thrice a week for 14 weeks) + Oral administration of DHA (125 mg/kg b.w. in 1 ml distilled water by oral gavage, thrice a week for 14 weeks on alternative days of DMBA application), GIV: Oral administration of DHA alone (125 mg/kg b.w. in 1 ml distilled water by oral gavage, thrice a week for 14 weeks). Results: Gross observations and histopathological findings revealed that, in GI: normal stratified squamous epithelium, in GII: well and moderately differentiated squamous cell carcinoma (SCC), in GIII: variable results ranges from hyperkeratosis, hyperkeratosis and focal hyperplasia, mild dysplasia, and well differentiated SCC with superficial invasion of tumor cells not extended to deeper areas, while in GIV: normal similar to GI. Immunohistochemical results indicated that oral DHA treatment to DMBA treated hamsters restored the normal expression of bcl-2. Conclusion: Our results indicated that DHA has the potential to be a dietary chemopreventive agent due to its capacity to improve carcinogen detoxification and to block/suppress the initiation and promotion stages of experimentally produced HBP carcinogenesis. |
| first_indexed | 2024-04-12T17:19:43Z |
| format | Article |
| id | doaj.art-40380c4799e04a8199f09283ea20b175 |
| institution | Directory Open Access Journal |
| issn | 2468-2942 |
| language | English |
| last_indexed | 2024-04-12T17:19:43Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cancer Treatment and Research Communications |
| spelling | doaj.art-40380c4799e04a8199f09283ea20b1752022-12-22T03:23:31ZengElsevierCancer Treatment and Research Communications2468-29422022-01-0131100558Effect of docosahexaenoic acid as a chemopreventive agent on experimentally induced hamster buccal pouch carcinogenesisEmad Mohamed Alqalshy0Amr Mohamed Ibrahim1Ahmed Abdel-Shakour Abdel-Hafiz2Kamal Abd El-Rahman Kamal3Magdy Alabasiry Alazzazi4Mohamed Refaat Omar5Amr Saad Abdel-Wahab6Saher Sayed Mohammed7Faculty of Dental Medicine (Boys-Cairo), AL- Azhar University, EgyptFaculty of Dental Medicine (Boys-Cairo), AL- Azhar University, Basic Dental Sciences Department, Faculty of Dentistry, Deraya University, New Miya, Minya, Egypt; Corresponding author.Faculty of Dental Medicine (Boys-Cairo), AL- Azhar University, EgyptFaculty of Dental Medicine (Boys-Cairo), AL- Azhar University, EgyptFaculty of Dental Medicine (Boys-Cairo), AL- Azhar University, Egypt; Oral Biology, College of Dentistry, The Islamic University, Najaf, IraqFaculty of Dentistry, Deraya University, New Miya, Minya, EgyptFaculty of Dental Medicine (Boys-Cairo), AL- Azhar University, EgyptFaculty of Dentistry, Minia University, EgyptPurpose: The current study was directed to investigate the effectiveness of docosahexaenoic acid (DHA) as a chemopreventive agent on experimentally induced hamster buccal pouch (HBP) carcinogenesis. Material and methods: In this study we used 40 Syrian male hamsters, five weeks old, were divided into 4 groups (GI, GII, GIII, and GIV) of 10 animals in each as follows, GI: Topical application of liquid paraffin alone (thrice a week for 14 weeks), GII: Topical application of 7, 12 dimethyl benz[a]anthracene (DMBA) alone (0.5% in liquid paraffin, thrice a week for 14 weeks), GIII: Topical application of DMBA (0.5% in liquid paraffin, thrice a week for 14 weeks) + Oral administration of DHA (125 mg/kg b.w. in 1 ml distilled water by oral gavage, thrice a week for 14 weeks on alternative days of DMBA application), GIV: Oral administration of DHA alone (125 mg/kg b.w. in 1 ml distilled water by oral gavage, thrice a week for 14 weeks). Results: Gross observations and histopathological findings revealed that, in GI: normal stratified squamous epithelium, in GII: well and moderately differentiated squamous cell carcinoma (SCC), in GIII: variable results ranges from hyperkeratosis, hyperkeratosis and focal hyperplasia, mild dysplasia, and well differentiated SCC with superficial invasion of tumor cells not extended to deeper areas, while in GIV: normal similar to GI. Immunohistochemical results indicated that oral DHA treatment to DMBA treated hamsters restored the normal expression of bcl-2. Conclusion: Our results indicated that DHA has the potential to be a dietary chemopreventive agent due to its capacity to improve carcinogen detoxification and to block/suppress the initiation and promotion stages of experimentally produced HBP carcinogenesis.http://www.sciencedirect.com/science/article/pii/S246829422200048XDocosahexaenoic acid (DHA)ChemopreventionBcl-2 familyExperimental studyHamster buccal pouch (HBP) Carcinogenesis |
| spellingShingle | Emad Mohamed Alqalshy Amr Mohamed Ibrahim Ahmed Abdel-Shakour Abdel-Hafiz Kamal Abd El-Rahman Kamal Magdy Alabasiry Alazzazi Mohamed Refaat Omar Amr Saad Abdel-Wahab Saher Sayed Mohammed Effect of docosahexaenoic acid as a chemopreventive agent on experimentally induced hamster buccal pouch carcinogenesis Cancer Treatment and Research Communications Docosahexaenoic acid (DHA) Chemoprevention Bcl-2 family Experimental study Hamster buccal pouch (HBP) Carcinogenesis |
| title | Effect of docosahexaenoic acid as a chemopreventive agent on experimentally induced hamster buccal pouch carcinogenesis |
| title_full | Effect of docosahexaenoic acid as a chemopreventive agent on experimentally induced hamster buccal pouch carcinogenesis |
| title_fullStr | Effect of docosahexaenoic acid as a chemopreventive agent on experimentally induced hamster buccal pouch carcinogenesis |
| title_full_unstemmed | Effect of docosahexaenoic acid as a chemopreventive agent on experimentally induced hamster buccal pouch carcinogenesis |
| title_short | Effect of docosahexaenoic acid as a chemopreventive agent on experimentally induced hamster buccal pouch carcinogenesis |
| title_sort | effect of docosahexaenoic acid as a chemopreventive agent on experimentally induced hamster buccal pouch carcinogenesis |
| topic | Docosahexaenoic acid (DHA) Chemoprevention Bcl-2 family Experimental study Hamster buccal pouch (HBP) Carcinogenesis |
| url | http://www.sciencedirect.com/science/article/pii/S246829422200048X |
| work_keys_str_mv | AT emadmohamedalqalshy effectofdocosahexaenoicacidasachemopreventiveagentonexperimentallyinducedhamsterbuccalpouchcarcinogenesis AT amrmohamedibrahim effectofdocosahexaenoicacidasachemopreventiveagentonexperimentallyinducedhamsterbuccalpouchcarcinogenesis AT ahmedabdelshakourabdelhafiz effectofdocosahexaenoicacidasachemopreventiveagentonexperimentallyinducedhamsterbuccalpouchcarcinogenesis AT kamalabdelrahmankamal effectofdocosahexaenoicacidasachemopreventiveagentonexperimentallyinducedhamsterbuccalpouchcarcinogenesis AT magdyalabasiryalazzazi effectofdocosahexaenoicacidasachemopreventiveagentonexperimentallyinducedhamsterbuccalpouchcarcinogenesis AT mohamedrefaatomar effectofdocosahexaenoicacidasachemopreventiveagentonexperimentallyinducedhamsterbuccalpouchcarcinogenesis AT amrsaadabdelwahab effectofdocosahexaenoicacidasachemopreventiveagentonexperimentallyinducedhamsterbuccalpouchcarcinogenesis AT sahersayedmohammed effectofdocosahexaenoicacidasachemopreventiveagentonexperimentallyinducedhamsterbuccalpouchcarcinogenesis |