Anti-Inflammatory Effects of <i>Mitrephora sirikitiae</i> Leaf Extract and Isolated Lignans in RAW 264.7 Cells
<i>Mitrephora sirikitiae</i> Weeras., Chalermglin & R.M.K. Saunders has been reported as a rich source of lignans that contribute to biological activities and health benefits. However, cellular anti-inflammatory effects of <i>M. sirikitiae</i> leaves and their lignan comp...
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| Format: | Article |
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MDPI AG
2022-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/10/3313 |
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| author | Supachoke Mangmool Chayaporn Limpichai Khine Kyi Han Vichai Reutrakul Natthinee Anantachoke |
| author_facet | Supachoke Mangmool Chayaporn Limpichai Khine Kyi Han Vichai Reutrakul Natthinee Anantachoke |
| author_sort | Supachoke Mangmool |
| collection | DOAJ |
| description | <i>Mitrephora sirikitiae</i> Weeras., Chalermglin & R.M.K. Saunders has been reported as a rich source of lignans that contribute to biological activities and health benefits. However, cellular anti-inflammatory effects of <i>M. sirikitiae</i> leaves and their lignan compounds have not been fully elucidated. Therefore, this study aimed to investigate the anti-inflammatory activities of methanol extract of <i>M. sirikitiae</i> leaves and their lignan constituents on lipopolysaccharide (LPS)-induced inflammation in RAW 264.7 mouse macrophage cells. Treatment of RAW 264.7 cells with the methanol extract of <i>M. sirikitiae</i> leaves and its isolated lignans, including (−)-phylligenin (<b>2</b>) and 3′,4-<i>O</i>-dimethylcedrusin (<b>6</b>) significantly decreased LPS-induced prostaglandin E<sub>2</sub> (PGE<sub>2</sub>) and nitric oxide (NO) productions. These inhibitory effects of the extract and isolated lignans on LPS-induced upregulation of PGE<sub>2</sub> and NO productions were derived from the suppression of cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS) production, respectively. In addition, treatment with 2-(3,4-dimethoxyphenyl)-6-(3,5-dimethoxyphenyl)-3,7-dioxabicyclo[3.3.0]octane (<b>3</b>) and mitrephoran (<b>5</b>) was able to suppress LPS-induced tumor necrosis factor alpha (TNF-α) secretion and synthesis in RAW 264.7 cells. These results demonstrated that <i>M. sirikitiae</i> leaves and some isolated lignans exhibited potent anti-inflammatory activity through the inhibition of secretion and synthesis of PGE<sub>2</sub>, NO, and TNF-α. |
| first_indexed | 2024-03-10T03:18:16Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T03:18:16Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-40402beaffcf4c12b03301c61d1e678c2023-11-23T12:24:27ZengMDPI AGMolecules1420-30492022-05-012710331310.3390/molecules27103313Anti-Inflammatory Effects of <i>Mitrephora sirikitiae</i> Leaf Extract and Isolated Lignans in RAW 264.7 CellsSupachoke Mangmool0Chayaporn Limpichai1Khine Kyi Han2Vichai Reutrakul3Natthinee Anantachoke4Department of Pharmacology, Faculty of Science, Mahidol University, Bangkok 10400, ThailandDepartment of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, ThailandDepartment of Pharmacology, University of Pharmacy, Yangon 11031, MyanmarDepartment of Chemistry, Faculty of Science, Mahidol University, Bangkok 10400, ThailandDepartment of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand<i>Mitrephora sirikitiae</i> Weeras., Chalermglin & R.M.K. Saunders has been reported as a rich source of lignans that contribute to biological activities and health benefits. However, cellular anti-inflammatory effects of <i>M. sirikitiae</i> leaves and their lignan compounds have not been fully elucidated. Therefore, this study aimed to investigate the anti-inflammatory activities of methanol extract of <i>M. sirikitiae</i> leaves and their lignan constituents on lipopolysaccharide (LPS)-induced inflammation in RAW 264.7 mouse macrophage cells. Treatment of RAW 264.7 cells with the methanol extract of <i>M. sirikitiae</i> leaves and its isolated lignans, including (−)-phylligenin (<b>2</b>) and 3′,4-<i>O</i>-dimethylcedrusin (<b>6</b>) significantly decreased LPS-induced prostaglandin E<sub>2</sub> (PGE<sub>2</sub>) and nitric oxide (NO) productions. These inhibitory effects of the extract and isolated lignans on LPS-induced upregulation of PGE<sub>2</sub> and NO productions were derived from the suppression of cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS) production, respectively. In addition, treatment with 2-(3,4-dimethoxyphenyl)-6-(3,5-dimethoxyphenyl)-3,7-dioxabicyclo[3.3.0]octane (<b>3</b>) and mitrephoran (<b>5</b>) was able to suppress LPS-induced tumor necrosis factor alpha (TNF-α) secretion and synthesis in RAW 264.7 cells. These results demonstrated that <i>M. sirikitiae</i> leaves and some isolated lignans exhibited potent anti-inflammatory activity through the inhibition of secretion and synthesis of PGE<sub>2</sub>, NO, and TNF-α.https://www.mdpi.com/1420-3049/27/10/3313<i>Mitrephora sirikitiae</i>anti-inflammationlignanspolyphenolsmRNA expressioncytokines |
| spellingShingle | Supachoke Mangmool Chayaporn Limpichai Khine Kyi Han Vichai Reutrakul Natthinee Anantachoke Anti-Inflammatory Effects of <i>Mitrephora sirikitiae</i> Leaf Extract and Isolated Lignans in RAW 264.7 Cells Molecules <i>Mitrephora sirikitiae</i> anti-inflammation lignans polyphenols mRNA expression cytokines |
| title | Anti-Inflammatory Effects of <i>Mitrephora sirikitiae</i> Leaf Extract and Isolated Lignans in RAW 264.7 Cells |
| title_full | Anti-Inflammatory Effects of <i>Mitrephora sirikitiae</i> Leaf Extract and Isolated Lignans in RAW 264.7 Cells |
| title_fullStr | Anti-Inflammatory Effects of <i>Mitrephora sirikitiae</i> Leaf Extract and Isolated Lignans in RAW 264.7 Cells |
| title_full_unstemmed | Anti-Inflammatory Effects of <i>Mitrephora sirikitiae</i> Leaf Extract and Isolated Lignans in RAW 264.7 Cells |
| title_short | Anti-Inflammatory Effects of <i>Mitrephora sirikitiae</i> Leaf Extract and Isolated Lignans in RAW 264.7 Cells |
| title_sort | anti inflammatory effects of i mitrephora sirikitiae i leaf extract and isolated lignans in raw 264 7 cells |
| topic | <i>Mitrephora sirikitiae</i> anti-inflammation lignans polyphenols mRNA expression cytokines |
| url | https://www.mdpi.com/1420-3049/27/10/3313 |
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