A Peak of H3T3 Phosphorylation Occurs in Synchrony with Mitosis in Sea Urchin Early Embryos
The sea urchin embryo provides a valuable system to analyse the molecular mechanisms orchestrating cell cycle progression and mitosis in a developmental context. However, although it is known that the regulation of histone activity by post-translational modification plays an important role during ce...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-04-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/9/4/898 |
| _version_ | 1797571319181606912 |
|---|---|
| author | Omid Feizbakhsh Florian Pontheaux Virginie Glippa Julia Morales Sandrine Ruchaud Patrick Cormier Fernando Roch |
| author_facet | Omid Feizbakhsh Florian Pontheaux Virginie Glippa Julia Morales Sandrine Ruchaud Patrick Cormier Fernando Roch |
| author_sort | Omid Feizbakhsh |
| collection | DOAJ |
| description | The sea urchin embryo provides a valuable system to analyse the molecular mechanisms orchestrating cell cycle progression and mitosis in a developmental context. However, although it is known that the regulation of histone activity by post-translational modification plays an important role during cell division, the dynamics and the impact of these modifications have not been characterised in detail in a developing embryo. Using different immuno-detection techniques, we show that the levels of Histone 3 phosphorylation at Threonine 3 oscillate in synchrony with mitosis in <i>Sphaerechinus granularis</i> early embryos. We present, in addition, the results of a pharmacological study aimed at analysing the role of this key histone post-translational modification during sea urchin early development. |
| first_indexed | 2024-03-10T20:37:38Z |
| format | Article |
| id | doaj.art-4040db13029a4c01b464cb25b87b2a39 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T20:37:38Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-4040db13029a4c01b464cb25b87b2a392023-11-19T20:52:13ZengMDPI AGCells2073-44092020-04-019489810.3390/cells9040898A Peak of H3T3 Phosphorylation Occurs in Synchrony with Mitosis in Sea Urchin Early EmbryosOmid Feizbakhsh0Florian Pontheaux1Virginie Glippa2Julia Morales3Sandrine Ruchaud4Patrick Cormier5Fernando Roch6Centre National de la Recherche Scientifique (CNRS), Sorbonne Université, Integrative Biology of Marine Models (LBI2M), Station Biologique de Roscoff, CS 90074, 29680 Roscoff CEDEX, FranceCentre National de la Recherche Scientifique (CNRS), Sorbonne Université, Integrative Biology of Marine Models (LBI2M), Station Biologique de Roscoff, CS 90074, 29680 Roscoff CEDEX, FranceCentre National de la Recherche Scientifique (CNRS), Sorbonne Université, Integrative Biology of Marine Models (LBI2M), Station Biologique de Roscoff, CS 90074, 29680 Roscoff CEDEX, FranceCentre National de la Recherche Scientifique (CNRS), Sorbonne Université, Integrative Biology of Marine Models (LBI2M), Station Biologique de Roscoff, CS 90074, 29680 Roscoff CEDEX, FranceCentre National de la Recherche Scientifique (CNRS), Sorbonne Université, Integrative Biology of Marine Models (LBI2M), Station Biologique de Roscoff, CS 90074, 29680 Roscoff CEDEX, FranceCentre National de la Recherche Scientifique (CNRS), Sorbonne Université, Integrative Biology of Marine Models (LBI2M), Station Biologique de Roscoff, CS 90074, 29680 Roscoff CEDEX, FranceCentre National de la Recherche Scientifique (CNRS), Sorbonne Université, Integrative Biology of Marine Models (LBI2M), Station Biologique de Roscoff, CS 90074, 29680 Roscoff CEDEX, FranceThe sea urchin embryo provides a valuable system to analyse the molecular mechanisms orchestrating cell cycle progression and mitosis in a developmental context. However, although it is known that the regulation of histone activity by post-translational modification plays an important role during cell division, the dynamics and the impact of these modifications have not been characterised in detail in a developing embryo. Using different immuno-detection techniques, we show that the levels of Histone 3 phosphorylation at Threonine 3 oscillate in synchrony with mitosis in <i>Sphaerechinus granularis</i> early embryos. We present, in addition, the results of a pharmacological study aimed at analysing the role of this key histone post-translational modification during sea urchin early development.https://www.mdpi.com/2073-4409/9/4/898histone phosphorylationHaspinmitosiscell divisionCDK1CHR-6494 |
| spellingShingle | Omid Feizbakhsh Florian Pontheaux Virginie Glippa Julia Morales Sandrine Ruchaud Patrick Cormier Fernando Roch A Peak of H3T3 Phosphorylation Occurs in Synchrony with Mitosis in Sea Urchin Early Embryos Cells histone phosphorylation Haspin mitosis cell division CDK1 CHR-6494 |
| title | A Peak of H3T3 Phosphorylation Occurs in Synchrony with Mitosis in Sea Urchin Early Embryos |
| title_full | A Peak of H3T3 Phosphorylation Occurs in Synchrony with Mitosis in Sea Urchin Early Embryos |
| title_fullStr | A Peak of H3T3 Phosphorylation Occurs in Synchrony with Mitosis in Sea Urchin Early Embryos |
| title_full_unstemmed | A Peak of H3T3 Phosphorylation Occurs in Synchrony with Mitosis in Sea Urchin Early Embryos |
| title_short | A Peak of H3T3 Phosphorylation Occurs in Synchrony with Mitosis in Sea Urchin Early Embryos |
| title_sort | peak of h3t3 phosphorylation occurs in synchrony with mitosis in sea urchin early embryos |
| topic | histone phosphorylation Haspin mitosis cell division CDK1 CHR-6494 |
| url | https://www.mdpi.com/2073-4409/9/4/898 |
| work_keys_str_mv | AT omidfeizbakhsh apeakofh3t3phosphorylationoccursinsynchronywithmitosisinseaurchinearlyembryos AT florianpontheaux apeakofh3t3phosphorylationoccursinsynchronywithmitosisinseaurchinearlyembryos AT virginieglippa apeakofh3t3phosphorylationoccursinsynchronywithmitosisinseaurchinearlyembryos AT juliamorales apeakofh3t3phosphorylationoccursinsynchronywithmitosisinseaurchinearlyembryos AT sandrineruchaud apeakofh3t3phosphorylationoccursinsynchronywithmitosisinseaurchinearlyembryos AT patrickcormier apeakofh3t3phosphorylationoccursinsynchronywithmitosisinseaurchinearlyembryos AT fernandoroch apeakofh3t3phosphorylationoccursinsynchronywithmitosisinseaurchinearlyembryos AT omidfeizbakhsh peakofh3t3phosphorylationoccursinsynchronywithmitosisinseaurchinearlyembryos AT florianpontheaux peakofh3t3phosphorylationoccursinsynchronywithmitosisinseaurchinearlyembryos AT virginieglippa peakofh3t3phosphorylationoccursinsynchronywithmitosisinseaurchinearlyembryos AT juliamorales peakofh3t3phosphorylationoccursinsynchronywithmitosisinseaurchinearlyembryos AT sandrineruchaud peakofh3t3phosphorylationoccursinsynchronywithmitosisinseaurchinearlyembryos AT patrickcormier peakofh3t3phosphorylationoccursinsynchronywithmitosisinseaurchinearlyembryos AT fernandoroch peakofh3t3phosphorylationoccursinsynchronywithmitosisinseaurchinearlyembryos |