The ability of febuxostat on periodontal inflammation and bone loss in rats with periodontitis by regulating nuclear factor-κb
We aimed to evaluate the effects of febuxostat on periodontal inflammation and bone loss in rats with periodontitis by regulating nuclear factor-κB (NF-κB). Sixty rats were randomly divided into sham (Group S), periodontitis model (Group P), febuxostat (Group F), minocycline hydrochloride (Group M),...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2023-06-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| Subjects: | |
| Online Access: | https://vetdergikafkas.org/pdf.php?id=3001 |
| _version_ | 1797724899370860544 |
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| author | Yanzhong YANG Cuiqiong MO Xiaohua TIAN Ying ZHENG Jie ZOU Chen CHEN |
| author_facet | Yanzhong YANG Cuiqiong MO Xiaohua TIAN Ying ZHENG Jie ZOU Chen CHEN |
| author_sort | Yanzhong YANG |
| collection | DOAJ |
| description | We aimed to evaluate the effects of febuxostat on periodontal inflammation and bone loss in rats with periodontitis by regulating
nuclear factor-κB (NF-κB). Sixty rats were randomly divided into sham (Group S), periodontitis model (Group P), febuxostat (Group F),
minocycline hydrochloride (Group M), NF-κB p65 inhibitor PDTC (Group C), and febuxostat + NF-κB p65 inhibitor PDTC (Group F+C)
groups (n=10). The levels of serum tumor necrosis factor-α (TNF-α), interleukin-17 (IL-17), and IL-10 were detected by enzyme-linked
immunosorbent assay. The pathological changes in periodontal tissues were detected by hematoxylin-eosin staining, and bone loss was
detected using micro-CT. Western blotting was performed to measure the protein expression of NF-κB p65 in periodontal tissues. Group
P had significantly higher levels of TNF-α and IL-17 and lower IL-10 levels than those of Group S (P<0.05). Group F had significantly
lower levels of serum TNF-α and IL-17 and higher IL-10 levels than those of Group P (P<0.05). The protein expression of NF-κB p65 in
the periodontal tissues of Group P was significantly higher than that of Group S, which was lower in Group F and M than that in Group P
(P<0.05). Compared with Group P, Group C had significantly lower levels of TNF-α and IL-17, CEJ-AC and protein expression of NF-κB
p65, but a higher level of IL-10 (P<0.05), and the trends were the same between Group F+C and Group F (P<0.05). Febuxostat can inhibit
periodontal inflammation and reduce alveolar bone loss in rats with periodontitis, probably by inhibiting the activation of NF-κB. |
| first_indexed | 2024-03-12T10:23:33Z |
| format | Article |
| id | doaj.art-404188b597264f7c9f7be6fe1f42b711 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-12T10:23:33Z |
| publishDate | 2023-06-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-404188b597264f7c9f7be6fe1f42b7112023-09-02T09:54:26ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512023-06-0129321321910.9775/kvfd.2022.287813001The ability of febuxostat on periodontal inflammation and bone loss in rats with periodontitis by regulating nuclear factor-κbYanzhong YANG0Cuiqiong MO1Xiaohua TIAN2Ying ZHENG3Jie ZOU4Chen CHEN5Department of Integrated Medicine, Hangzhou Stomatology Hospital, 310000, Hangzhou, Zhejiang Province, P.R. CHINAThe 903rd Hospital of the Joint Logistics Support Force of PLA, 310000, Hangzhou, Zhejiang Province, P.R. CHINADepartment of Integrated Medicine, Hangzhou Stomatology Hospital, 310000, Hangzhou, Zhejiang Province, P.R. CHINAThe 903rd Hospital of the Joint Logistics Support Force of PLA, 310000, Hangzhou, Zhejiang Province, P.R. CHINAThe 903rd Hospital of the Joint Logistics Support Force of PLA, 310000, Hangzhou, Zhejiang Province, P.R. CHINAZhuji Affiliated Hospital of Wenzhou Medical University, 311800, Zhuji, Zhejiang Province, P.R. CHINAWe aimed to evaluate the effects of febuxostat on periodontal inflammation and bone loss in rats with periodontitis by regulating nuclear factor-κB (NF-κB). Sixty rats were randomly divided into sham (Group S), periodontitis model (Group P), febuxostat (Group F), minocycline hydrochloride (Group M), NF-κB p65 inhibitor PDTC (Group C), and febuxostat + NF-κB p65 inhibitor PDTC (Group F+C) groups (n=10). The levels of serum tumor necrosis factor-α (TNF-α), interleukin-17 (IL-17), and IL-10 were detected by enzyme-linked immunosorbent assay. The pathological changes in periodontal tissues were detected by hematoxylin-eosin staining, and bone loss was detected using micro-CT. Western blotting was performed to measure the protein expression of NF-κB p65 in periodontal tissues. Group P had significantly higher levels of TNF-α and IL-17 and lower IL-10 levels than those of Group S (P<0.05). Group F had significantly lower levels of serum TNF-α and IL-17 and higher IL-10 levels than those of Group P (P<0.05). The protein expression of NF-κB p65 in the periodontal tissues of Group P was significantly higher than that of Group S, which was lower in Group F and M than that in Group P (P<0.05). Compared with Group P, Group C had significantly lower levels of TNF-α and IL-17, CEJ-AC and protein expression of NF-κB p65, but a higher level of IL-10 (P<0.05), and the trends were the same between Group F+C and Group F (P<0.05). Febuxostat can inhibit periodontal inflammation and reduce alveolar bone loss in rats with periodontitis, probably by inhibiting the activation of NF-κB.https://vetdergikafkas.org/pdf.php?id=3001febuxostatalveolar bone lossperiodontitisnf-κb |
| spellingShingle | Yanzhong YANG Cuiqiong MO Xiaohua TIAN Ying ZHENG Jie ZOU Chen CHEN The ability of febuxostat on periodontal inflammation and bone loss in rats with periodontitis by regulating nuclear factor-κb Kafkas Universitesi Veteriner Fakültesi Dergisi febuxostat alveolar bone loss periodontitis nf-κb |
| title | The ability of febuxostat on periodontal inflammation and bone loss in rats with periodontitis by regulating nuclear factor-κb |
| title_full | The ability of febuxostat on periodontal inflammation and bone loss in rats with periodontitis by regulating nuclear factor-κb |
| title_fullStr | The ability of febuxostat on periodontal inflammation and bone loss in rats with periodontitis by regulating nuclear factor-κb |
| title_full_unstemmed | The ability of febuxostat on periodontal inflammation and bone loss in rats with periodontitis by regulating nuclear factor-κb |
| title_short | The ability of febuxostat on periodontal inflammation and bone loss in rats with periodontitis by regulating nuclear factor-κb |
| title_sort | ability of febuxostat on periodontal inflammation and bone loss in rats with periodontitis by regulating nuclear factor κb |
| topic | febuxostat alveolar bone loss periodontitis nf-κb |
| url | https://vetdergikafkas.org/pdf.php?id=3001 |
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