Spontaneous Osteogenic Differentiation of Human Mesenchymal Stem Cells by Tuna-Bone-Derived Hydroxyapatite Composites with Green Tea Polyphenol-Reduced Graphene Oxide
In recent years, bone tissue engineering (BTE) has made significant progress in promoting the direct and functional connection between bone and graft, including osseointegration and osteoconduction, to facilitate the healing of damaged bone tissues. Herein, we introduce a new, environmentally friend...
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MDPI AG
2023-05-01
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| Online Access: | https://www.mdpi.com/2073-4409/12/11/1448 |
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| author | Moon Sung Kang Rowoon Park Hyo Jung Jo Yong Cheol Shin Chang-Seok Kim Suong-Hyu Hyon Suck Won Hong Junghwan Oh Dong-Wook Han |
| author_facet | Moon Sung Kang Rowoon Park Hyo Jung Jo Yong Cheol Shin Chang-Seok Kim Suong-Hyu Hyon Suck Won Hong Junghwan Oh Dong-Wook Han |
| author_sort | Moon Sung Kang |
| collection | DOAJ |
| description | In recent years, bone tissue engineering (BTE) has made significant progress in promoting the direct and functional connection between bone and graft, including osseointegration and osteoconduction, to facilitate the healing of damaged bone tissues. Herein, we introduce a new, environmentally friendly, and cost-effective method for synthesizing reduced graphene oxide (rGO) and hydroxyapatite (HAp). The method uses epigallocatechin-3-<i>O</i>-gallate (EGCG) as a reducing agent to synthesize rGO (E-rGO), and HAp powder is obtained from Atlantic bluefin tuna (<i>Thunnus thynnus</i>). The physicochemical analysis indicated that the E-rGO/HAp composites had exceptional properties for use as BTE scaffolds, as well as high purity. Moreover, we discovered that E-rGO/HAp composites facilitated not only the proliferation, but also early and late osteogenic differentiation of human mesenchymal stem cells (hMSCs). Our work suggests that E-rGO/HAp composites may play a significant role in promoting the spontaneous osteogenic differentiation of hMSCs, and we envision that E-rGO/HAp composites could serve as promising candidates for BTE scaffolds, stem-cell differentiation stimulators, and implantable device components because of their biocompatible and bioactive properties. Overall, we suggest a new approach for developing cost-effective and environmentally friendly E-rGO/HAp composite materials for BTE application. |
| first_indexed | 2024-03-11T03:10:24Z |
| format | Article |
| id | doaj.art-4042c0110ab841e48da3b73a64f70ef6 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-11T03:10:24Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-4042c0110ab841e48da3b73a64f70ef62023-11-18T07:40:15ZengMDPI AGCells2073-44092023-05-011211144810.3390/cells12111448Spontaneous Osteogenic Differentiation of Human Mesenchymal Stem Cells by Tuna-Bone-Derived Hydroxyapatite Composites with Green Tea Polyphenol-Reduced Graphene OxideMoon Sung Kang0Rowoon Park1Hyo Jung Jo2Yong Cheol Shin3Chang-Seok Kim4Suong-Hyu Hyon5Suck Won Hong6Junghwan Oh7Dong-Wook Han8Department of Cogno-Mechatronics Engineering, College of Nanoscience and Nanotechnology, Pusan National University, Busan 46241, Republic of KoreaDepartment of Cogno-Mechatronics Engineering, College of Nanoscience and Nanotechnology, Pusan National University, Busan 46241, Republic of KoreaDepartment of Cogno-Mechatronics Engineering, College of Nanoscience and Nanotechnology, Pusan National University, Busan 46241, Republic of KoreaDepartment of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USADepartment of Cogno-Mechatronics Engineering, College of Nanoscience and Nanotechnology, Pusan National University, Busan 46241, Republic of KoreaBMG Inc., Kyoto 601-8023, JapanDepartment of Cogno-Mechatronics Engineering, College of Nanoscience and Nanotechnology, Pusan National University, Busan 46241, Republic of KoreaIndustry 4.0 Convergence Bionics Engineering, Pukyong National University, Busan 48513, Republic of KoreaDepartment of Cogno-Mechatronics Engineering, College of Nanoscience and Nanotechnology, Pusan National University, Busan 46241, Republic of KoreaIn recent years, bone tissue engineering (BTE) has made significant progress in promoting the direct and functional connection between bone and graft, including osseointegration and osteoconduction, to facilitate the healing of damaged bone tissues. Herein, we introduce a new, environmentally friendly, and cost-effective method for synthesizing reduced graphene oxide (rGO) and hydroxyapatite (HAp). The method uses epigallocatechin-3-<i>O</i>-gallate (EGCG) as a reducing agent to synthesize rGO (E-rGO), and HAp powder is obtained from Atlantic bluefin tuna (<i>Thunnus thynnus</i>). The physicochemical analysis indicated that the E-rGO/HAp composites had exceptional properties for use as BTE scaffolds, as well as high purity. Moreover, we discovered that E-rGO/HAp composites facilitated not only the proliferation, but also early and late osteogenic differentiation of human mesenchymal stem cells (hMSCs). Our work suggests that E-rGO/HAp composites may play a significant role in promoting the spontaneous osteogenic differentiation of hMSCs, and we envision that E-rGO/HAp composites could serve as promising candidates for BTE scaffolds, stem-cell differentiation stimulators, and implantable device components because of their biocompatible and bioactive properties. Overall, we suggest a new approach for developing cost-effective and environmentally friendly E-rGO/HAp composite materials for BTE application.https://www.mdpi.com/2073-4409/12/11/1448bone tissue engineeringhydroxyapatitereduced graphene oxidehuman mesenchymal stem cellosteogenic differentiation |
| spellingShingle | Moon Sung Kang Rowoon Park Hyo Jung Jo Yong Cheol Shin Chang-Seok Kim Suong-Hyu Hyon Suck Won Hong Junghwan Oh Dong-Wook Han Spontaneous Osteogenic Differentiation of Human Mesenchymal Stem Cells by Tuna-Bone-Derived Hydroxyapatite Composites with Green Tea Polyphenol-Reduced Graphene Oxide Cells bone tissue engineering hydroxyapatite reduced graphene oxide human mesenchymal stem cell osteogenic differentiation |
| title | Spontaneous Osteogenic Differentiation of Human Mesenchymal Stem Cells by Tuna-Bone-Derived Hydroxyapatite Composites with Green Tea Polyphenol-Reduced Graphene Oxide |
| title_full | Spontaneous Osteogenic Differentiation of Human Mesenchymal Stem Cells by Tuna-Bone-Derived Hydroxyapatite Composites with Green Tea Polyphenol-Reduced Graphene Oxide |
| title_fullStr | Spontaneous Osteogenic Differentiation of Human Mesenchymal Stem Cells by Tuna-Bone-Derived Hydroxyapatite Composites with Green Tea Polyphenol-Reduced Graphene Oxide |
| title_full_unstemmed | Spontaneous Osteogenic Differentiation of Human Mesenchymal Stem Cells by Tuna-Bone-Derived Hydroxyapatite Composites with Green Tea Polyphenol-Reduced Graphene Oxide |
| title_short | Spontaneous Osteogenic Differentiation of Human Mesenchymal Stem Cells by Tuna-Bone-Derived Hydroxyapatite Composites with Green Tea Polyphenol-Reduced Graphene Oxide |
| title_sort | spontaneous osteogenic differentiation of human mesenchymal stem cells by tuna bone derived hydroxyapatite composites with green tea polyphenol reduced graphene oxide |
| topic | bone tissue engineering hydroxyapatite reduced graphene oxide human mesenchymal stem cell osteogenic differentiation |
| url | https://www.mdpi.com/2073-4409/12/11/1448 |
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