Recognition of Pep-13/25 MAMPs of Phytophthora localizes to an RLK locus in Solanum microdontum
Pattern-triggered immunity (PTI) in plants is mediated by cell surface-localized pattern recognition receptors (PRRs) upon perception of microbe-associated molecular pattern (MAMPs). MAMPs are conserved molecules across microbe species, or even kingdoms, and PRRs can confer broad-spectrum disease re...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1037030/full |
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| author | Xiao Lin Yerisf Carla Torres Ascurra Happyka Fillianti Laura Dethier Laura de Rond Emmanouil Domazakis Carolina Aguilera-Galvez Afewerki Yohannes Kiros Evert Jacobsen Richard G. F. Visser Thorsten Nürnberger Thorsten Nürnberger Vivianne G. A. A. Vleeshouwers |
| author_facet | Xiao Lin Yerisf Carla Torres Ascurra Happyka Fillianti Laura Dethier Laura de Rond Emmanouil Domazakis Carolina Aguilera-Galvez Afewerki Yohannes Kiros Evert Jacobsen Richard G. F. Visser Thorsten Nürnberger Thorsten Nürnberger Vivianne G. A. A. Vleeshouwers |
| author_sort | Xiao Lin |
| collection | DOAJ |
| description | Pattern-triggered immunity (PTI) in plants is mediated by cell surface-localized pattern recognition receptors (PRRs) upon perception of microbe-associated molecular pattern (MAMPs). MAMPs are conserved molecules across microbe species, or even kingdoms, and PRRs can confer broad-spectrum disease resistance. Pep-13/25 are well-characterized MAMPs in Phytophthora species, which are renowned devastating oomycete pathogens of potato and other plants, and for which genetic resistance is highly wanted. Pep-13/25 are derived from a 42 kDa transglutaminase GP42, but their cognate PRR has remained unknown. Here, we genetically mapped a novel surface immune receptor that recognizes Pep-25. By using effectoromics screening, we characterized the recognition spectrum of Pep-13/25 in diverse Solanaceae species. Response to Pep-13/25 was predominantly found in potato and related wild tuber-bearing Solanum species. Bulk-segregant RNA sequencing (BSR-Seq) and genetic mapping the response to Pep-25 led to a 0.081 cM region on the top of chromosome 3 in the wild potato species Solanum microdontum subsp. gigantophyllum. Some BAC clones in this region were isolated and sequenced, and we found the Pep-25 receptor locates in a complex receptor-like kinase (RLK) locus. This study is an important step toward the identification of the Pep-13/25 receptor, which can potentially lead to broad application in potato and various other hosts of Phytophthora species |
| first_indexed | 2024-04-10T23:20:44Z |
| format | Article |
| id | doaj.art-40461ce1aca4476ca945f10f48b8ad5a |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-10T23:20:44Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-40461ce1aca4476ca945f10f48b8ad5a2023-01-12T14:52:05ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-01-011310.3389/fpls.2022.10370301037030Recognition of Pep-13/25 MAMPs of Phytophthora localizes to an RLK locus in Solanum microdontumXiao Lin0Yerisf Carla Torres Ascurra1Happyka Fillianti2Laura Dethier3Laura de Rond4Emmanouil Domazakis5Carolina Aguilera-Galvez6Afewerki Yohannes Kiros7Evert Jacobsen8Richard G. F. Visser9Thorsten Nürnberger10Thorsten Nürnberger11Vivianne G. A. A. Vleeshouwers12Plant Breeding, Wageningen University and Research, Wageningen, NetherlandsPlant Breeding, Wageningen University and Research, Wageningen, NetherlandsPlant Breeding, Wageningen University and Research, Wageningen, NetherlandsPlant Breeding, Wageningen University and Research, Wageningen, NetherlandsPlant Breeding, Wageningen University and Research, Wageningen, NetherlandsPlant Breeding, Wageningen University and Research, Wageningen, NetherlandsPlant Breeding, Wageningen University and Research, Wageningen, NetherlandsPlant Breeding, Wageningen University and Research, Wageningen, NetherlandsPlant Breeding, Wageningen University and Research, Wageningen, NetherlandsPlant Breeding, Wageningen University and Research, Wageningen, NetherlandsDepartment of Plant Biochemistry, Centre of Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, GermanyDepartment of Biochemistry, University of Johannesburg, Johannesburg, South AfricaPlant Breeding, Wageningen University and Research, Wageningen, NetherlandsPattern-triggered immunity (PTI) in plants is mediated by cell surface-localized pattern recognition receptors (PRRs) upon perception of microbe-associated molecular pattern (MAMPs). MAMPs are conserved molecules across microbe species, or even kingdoms, and PRRs can confer broad-spectrum disease resistance. Pep-13/25 are well-characterized MAMPs in Phytophthora species, which are renowned devastating oomycete pathogens of potato and other plants, and for which genetic resistance is highly wanted. Pep-13/25 are derived from a 42 kDa transglutaminase GP42, but their cognate PRR has remained unknown. Here, we genetically mapped a novel surface immune receptor that recognizes Pep-25. By using effectoromics screening, we characterized the recognition spectrum of Pep-13/25 in diverse Solanaceae species. Response to Pep-13/25 was predominantly found in potato and related wild tuber-bearing Solanum species. Bulk-segregant RNA sequencing (BSR-Seq) and genetic mapping the response to Pep-25 led to a 0.081 cM region on the top of chromosome 3 in the wild potato species Solanum microdontum subsp. gigantophyllum. Some BAC clones in this region were isolated and sequenced, and we found the Pep-25 receptor locates in a complex receptor-like kinase (RLK) locus. This study is an important step toward the identification of the Pep-13/25 receptor, which can potentially lead to broad application in potato and various other hosts of Phytophthora specieshttps://www.frontiersin.org/articles/10.3389/fpls.2022.1037030/fullPotato late blightPhytophthora infestansMAMPRLKPep-13/25BSR-Seq |
| spellingShingle | Xiao Lin Yerisf Carla Torres Ascurra Happyka Fillianti Laura Dethier Laura de Rond Emmanouil Domazakis Carolina Aguilera-Galvez Afewerki Yohannes Kiros Evert Jacobsen Richard G. F. Visser Thorsten Nürnberger Thorsten Nürnberger Vivianne G. A. A. Vleeshouwers Recognition of Pep-13/25 MAMPs of Phytophthora localizes to an RLK locus in Solanum microdontum Frontiers in Plant Science Potato late blight Phytophthora infestans MAMP RLK Pep-13/25 BSR-Seq |
| title | Recognition of Pep-13/25 MAMPs of Phytophthora localizes to an RLK locus in Solanum microdontum |
| title_full | Recognition of Pep-13/25 MAMPs of Phytophthora localizes to an RLK locus in Solanum microdontum |
| title_fullStr | Recognition of Pep-13/25 MAMPs of Phytophthora localizes to an RLK locus in Solanum microdontum |
| title_full_unstemmed | Recognition of Pep-13/25 MAMPs of Phytophthora localizes to an RLK locus in Solanum microdontum |
| title_short | Recognition of Pep-13/25 MAMPs of Phytophthora localizes to an RLK locus in Solanum microdontum |
| title_sort | recognition of pep 13 25 mamps of phytophthora localizes to an rlk locus in solanum microdontum |
| topic | Potato late blight Phytophthora infestans MAMP RLK Pep-13/25 BSR-Seq |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1037030/full |
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