Lasp1 Expression Is Implicated in Embryonic Development of Zebrafish
The LIM and SH3 domain protein 1 (LASP1) was originally identified in metastatic breast cancer and mainly characterized as a cytoskeleton protein overexpressed in various cancer types. At present, little is known about LASP1 expression in physiological conditions, and its function during embryonic d...
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MDPI AG
2022-12-01
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| Online Access: | https://www.mdpi.com/2073-4425/14/1/35 |
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| author | Ilaria Grossi Marco Schiavone Elena Cannone Oana Andreea Grejdan Chiara Tobia Francesca Bonomini Rita Rezzani Alessandro Salvi Giuseppina De Petro |
| author_facet | Ilaria Grossi Marco Schiavone Elena Cannone Oana Andreea Grejdan Chiara Tobia Francesca Bonomini Rita Rezzani Alessandro Salvi Giuseppina De Petro |
| author_sort | Ilaria Grossi |
| collection | DOAJ |
| description | The LIM and SH3 domain protein 1 (LASP1) was originally identified in metastatic breast cancer and mainly characterized as a cytoskeleton protein overexpressed in various cancer types. At present, little is known about LASP1 expression in physiological conditions, and its function during embryonic development has not been elucidated. Here, we focused on Lasp1 and embryonic development, choosing zebrafish as a vertebrate model. For the first time, we identified and determined the expression of Lasp1 protein at various stages of development, at 48 and 72 h post-fertilization (hpf), at 6 days pf and in different organs of zebrafish adults by Western blotting, 3D light-sheet microscopy and fluorescent immunohistochemistry. Further, we showed that specific <i>lasp1</i> morpholino (MO) led to (i) abnormal morphants with alterations in several organs, (ii) effective knockdown of endogenous Lasp1 protein and (iii) an increase in <i>lasp1</i> mRNA, as detected by ddPCR. The co-injection of <i>lasp1</i> mRNA with <i>lasp1</i> MO partially rescued morphant phenotypes, thus confirming the specificity of the MO oligonucleotide-induced defects. We also detected an increase in apoptosis following <i>lasp1</i> MO treatment. Our results suggest a significant role for Lasp1 in embryonic development, highlighting zebrafish as a vertebrate model suitable for studying Lasp1 function in developmental biology and organogenesis. |
| first_indexed | 2024-03-09T12:37:45Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T12:37:45Z |
| publishDate | 2022-12-01 |
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| record_format | Article |
| series | Genes |
| spelling | doaj.art-404787c410964b0a89ecabb87e8744d12023-11-30T22:22:34ZengMDPI AGGenes2073-44252022-12-011413510.3390/genes14010035Lasp1 Expression Is Implicated in Embryonic Development of ZebrafishIlaria Grossi0Marco Schiavone1Elena Cannone2Oana Andreea Grejdan3Chiara Tobia4Francesca Bonomini5Rita Rezzani6Alessandro Salvi7Giuseppina De Petro8Department of Molecular and Translational Medicine, Division of Biology and Genetics, University of Brescia, 25123 Brescia, ItalyDepartment of Molecular and Translational Medicine, Division of Biology and Genetics, University of Brescia, 25123 Brescia, ItalyDepartment of Molecular and Translational Medicine, Division of Biology and Genetics, University of Brescia, 25123 Brescia, ItalyDepartment of Molecular and Translational Medicine, Division of Biology and Genetics, University of Brescia, 25123 Brescia, ItalyDepartment of Molecular and Translational Medicine, Division of Experimental Oncology and Immunology, University of Brescia, 25123 Brescia, ItalyDepartment of Clinical and Experimental Sciences, Division of Anatomy and Physiopathology, University of Brescia, 25123 Brescia, ItalyDepartment of Clinical and Experimental Sciences, Division of Anatomy and Physiopathology, University of Brescia, 25123 Brescia, ItalyDepartment of Molecular and Translational Medicine, Division of Biology and Genetics, University of Brescia, 25123 Brescia, ItalyDepartment of Molecular and Translational Medicine, Division of Biology and Genetics, University of Brescia, 25123 Brescia, ItalyThe LIM and SH3 domain protein 1 (LASP1) was originally identified in metastatic breast cancer and mainly characterized as a cytoskeleton protein overexpressed in various cancer types. At present, little is known about LASP1 expression in physiological conditions, and its function during embryonic development has not been elucidated. Here, we focused on Lasp1 and embryonic development, choosing zebrafish as a vertebrate model. For the first time, we identified and determined the expression of Lasp1 protein at various stages of development, at 48 and 72 h post-fertilization (hpf), at 6 days pf and in different organs of zebrafish adults by Western blotting, 3D light-sheet microscopy and fluorescent immunohistochemistry. Further, we showed that specific <i>lasp1</i> morpholino (MO) led to (i) abnormal morphants with alterations in several organs, (ii) effective knockdown of endogenous Lasp1 protein and (iii) an increase in <i>lasp1</i> mRNA, as detected by ddPCR. The co-injection of <i>lasp1</i> mRNA with <i>lasp1</i> MO partially rescued morphant phenotypes, thus confirming the specificity of the MO oligonucleotide-induced defects. We also detected an increase in apoptosis following <i>lasp1</i> MO treatment. Our results suggest a significant role for Lasp1 in embryonic development, highlighting zebrafish as a vertebrate model suitable for studying Lasp1 function in developmental biology and organogenesis.https://www.mdpi.com/2073-4425/14/1/35Lasp1 expressionzebrafish modelembryonic developmentapoptosis |
| spellingShingle | Ilaria Grossi Marco Schiavone Elena Cannone Oana Andreea Grejdan Chiara Tobia Francesca Bonomini Rita Rezzani Alessandro Salvi Giuseppina De Petro Lasp1 Expression Is Implicated in Embryonic Development of Zebrafish Genes Lasp1 expression zebrafish model embryonic development apoptosis |
| title | Lasp1 Expression Is Implicated in Embryonic Development of Zebrafish |
| title_full | Lasp1 Expression Is Implicated in Embryonic Development of Zebrafish |
| title_fullStr | Lasp1 Expression Is Implicated in Embryonic Development of Zebrafish |
| title_full_unstemmed | Lasp1 Expression Is Implicated in Embryonic Development of Zebrafish |
| title_short | Lasp1 Expression Is Implicated in Embryonic Development of Zebrafish |
| title_sort | lasp1 expression is implicated in embryonic development of zebrafish |
| topic | Lasp1 expression zebrafish model embryonic development apoptosis |
| url | https://www.mdpi.com/2073-4425/14/1/35 |
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