Antioxidant Intervention Attenuates Aging-Related Changes in the Murine Ovary and Oocyte
Advanced maternal age (AMA) is associated with reduced fertility due in part to diminished ovarian follicle quantity, inferior oocyte quality, chromosome aneuploidy, and lower implantation rates. Ovarian aging is accompanied by increased oxidative stress and blunted antioxidant signaling, such that...
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MDPI AG
2020-10-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/10/11/250 |
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| author | Mandy G. Katz-Jaffe Sydney L. Lane Jason C. Parks Blair R. McCallie Rachel Makloski William B. Schoolcraft |
| author_facet | Mandy G. Katz-Jaffe Sydney L. Lane Jason C. Parks Blair R. McCallie Rachel Makloski William B. Schoolcraft |
| author_sort | Mandy G. Katz-Jaffe |
| collection | DOAJ |
| description | Advanced maternal age (AMA) is associated with reduced fertility due in part to diminished ovarian follicle quantity, inferior oocyte quality, chromosome aneuploidy, and lower implantation rates. Ovarian aging is accompanied by increased oxidative stress and blunted antioxidant signaling, such that antioxidant intervention could improve reproductive potential. The first aim of this study was to determine the molecular effects of antioxidant intervention in the ovaries and oocytes of aged mice, utilizing a supplement containing only naturally occurring açaí (Euterpe oleracea) with an oxygen radical absorbance capacity of 208,628 μmol Trolox equivalent (TE)/100 g indicating high antioxidant activity. Nine month old female CF-1 mice were administered 80 mg/day antioxidants (<i>n</i> = 12) or standard diet (<i>n</i> = 12) for 12 weeks. In the ovary, antioxidant treatment upregulated β-adrenergic signaling, downregulated apoptosis and proinflammatory signaling, and variably affected cell growth and antioxidant pathways (<i>p</i> < 0.05). Exogenous antioxidants also increased the oocyte expression of antioxidant genes <i>GPX1</i>, <i>SOD2</i>, and <i>GSR</i> (<i>p</i> < 0.05). A feasibility analysis was then conducted on female AMA infertility patients as a proof-of-principle investigation. Patients (<i>n</i> = 121; <45 years old) consented to receiving 600 mg antioxidants three times daily for ≥8 weeks preceding infertility treatment. Preliminary results indicate promising outcomes for AMA patients, warranting further investigation. |
| first_indexed | 2024-03-10T15:25:28Z |
| format | Article |
| id | doaj.art-404e113f06cb4a65a84f0027d3f5d4dd |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T15:25:28Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-404e113f06cb4a65a84f0027d3f5d4dd2023-11-20T18:06:18ZengMDPI AGLife2075-17292020-10-01101125010.3390/life10110250Antioxidant Intervention Attenuates Aging-Related Changes in the Murine Ovary and OocyteMandy G. Katz-Jaffe0Sydney L. Lane1Jason C. Parks2Blair R. McCallie3Rachel Makloski4William B. Schoolcraft5Colorado Center for Reproductive Medicine, Lone Tree, CO 80124, USAColorado Center for Reproductive Medicine, Lone Tree, CO 80124, USAColorado Center for Reproductive Medicine, Lone Tree, CO 80124, USAColorado Center for Reproductive Medicine, Lone Tree, CO 80124, USAColorado Center for Reproductive Medicine, Lone Tree, CO 80124, USAColorado Center for Reproductive Medicine, Lone Tree, CO 80124, USAAdvanced maternal age (AMA) is associated with reduced fertility due in part to diminished ovarian follicle quantity, inferior oocyte quality, chromosome aneuploidy, and lower implantation rates. Ovarian aging is accompanied by increased oxidative stress and blunted antioxidant signaling, such that antioxidant intervention could improve reproductive potential. The first aim of this study was to determine the molecular effects of antioxidant intervention in the ovaries and oocytes of aged mice, utilizing a supplement containing only naturally occurring açaí (Euterpe oleracea) with an oxygen radical absorbance capacity of 208,628 μmol Trolox equivalent (TE)/100 g indicating high antioxidant activity. Nine month old female CF-1 mice were administered 80 mg/day antioxidants (<i>n</i> = 12) or standard diet (<i>n</i> = 12) for 12 weeks. In the ovary, antioxidant treatment upregulated β-adrenergic signaling, downregulated apoptosis and proinflammatory signaling, and variably affected cell growth and antioxidant pathways (<i>p</i> < 0.05). Exogenous antioxidants also increased the oocyte expression of antioxidant genes <i>GPX1</i>, <i>SOD2</i>, and <i>GSR</i> (<i>p</i> < 0.05). A feasibility analysis was then conducted on female AMA infertility patients as a proof-of-principle investigation. Patients (<i>n</i> = 121; <45 years old) consented to receiving 600 mg antioxidants three times daily for ≥8 weeks preceding infertility treatment. Preliminary results indicate promising outcomes for AMA patients, warranting further investigation.https://www.mdpi.com/2075-1729/10/11/250antioxidantsflavonoidsoxidative stressadvanced maternal agein vitro fertilizationdietary supplements |
| spellingShingle | Mandy G. Katz-Jaffe Sydney L. Lane Jason C. Parks Blair R. McCallie Rachel Makloski William B. Schoolcraft Antioxidant Intervention Attenuates Aging-Related Changes in the Murine Ovary and Oocyte Life antioxidants flavonoids oxidative stress advanced maternal age in vitro fertilization dietary supplements |
| title | Antioxidant Intervention Attenuates Aging-Related Changes in the Murine Ovary and Oocyte |
| title_full | Antioxidant Intervention Attenuates Aging-Related Changes in the Murine Ovary and Oocyte |
| title_fullStr | Antioxidant Intervention Attenuates Aging-Related Changes in the Murine Ovary and Oocyte |
| title_full_unstemmed | Antioxidant Intervention Attenuates Aging-Related Changes in the Murine Ovary and Oocyte |
| title_short | Antioxidant Intervention Attenuates Aging-Related Changes in the Murine Ovary and Oocyte |
| title_sort | antioxidant intervention attenuates aging related changes in the murine ovary and oocyte |
| topic | antioxidants flavonoids oxidative stress advanced maternal age in vitro fertilization dietary supplements |
| url | https://www.mdpi.com/2075-1729/10/11/250 |
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