The FBA Motif-Containing Protein <i>NpFBA1</i> Causes Leaf Curling and Reduces Resistance to Black Shank Disease in Tobacco
Plant leaf morphology has a great impact on plant drought resistance, ornamental research and leaf yield. In this study, we identified a new gene in <i>Nicotiana plumbaginifolia</i>, <i>NpFBA1</i>, that causes leaf curl. The results show that the <i>NpFBA1</i> pro...
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| Format: | Article |
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MDPI AG
2021-12-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/11/12/2478 |
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| author | Zhongyi Xie Guo Wen Yao Yang Haiyan Wang Jinying Wang Chenggong Lei Qigao Guo Jiangbo Dang Guolu Liang |
| author_facet | Zhongyi Xie Guo Wen Yao Yang Haiyan Wang Jinying Wang Chenggong Lei Qigao Guo Jiangbo Dang Guolu Liang |
| author_sort | Zhongyi Xie |
| collection | DOAJ |
| description | Plant leaf morphology has a great impact on plant drought resistance, ornamental research and leaf yield. In this study, we identified a new gene in <i>Nicotiana plumbaginifolia</i>, <i>NpFBA1</i>, that causes leaf curl. The results show that the <i>NpFBA1</i> protein contains only one unique F-box associated (FBA) domain and does not have an F-box conserved domain. Phylogenetic analysis placed this gene and other Nicotiana FBA genes on a separate branch, and the <i>NpFBA1</i> protein localized to the nucleus and cytoplasm. The expression of <i>NpFBA1</i> was induced by black shank pathogen (<i>Phytophthora parasitica</i> var. <i>nicotianae</i>) infection and treatment with salicylic acid (SA) and methyl jasmonate (MeJA). <i>NpFBA1</i>-overexpressing transgenic lines showed leaf curling and aging during the rosette phase. During the bolting period, the leaves were curly and rounded, and the plants were dwarfed. In addition, <i>NpFBA1</i>-overexpressing lines were more susceptible to disease than wild-type (WT) plants. Further studies revealed that overexpression of <i>NpFBA1</i> significantly downregulated the expression of auxin response factors such as <i>NtARF3</i> and the lignin synthesis genes <i>NtPAL</i>, <i>NtC4H</i>, <i>NtCAD2</i>, and <i>NtCCR1</i> in the leaves. In conclusion, <i>NpFBA1</i> may play a key role in regulating leaf development and the response to pathogen infection. |
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| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T04:40:20Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-4051d6792e484aa1a81b03cf8e478ac62023-11-23T03:22:30ZengMDPI AGAgronomy2073-43952021-12-011112247810.3390/agronomy11122478The FBA Motif-Containing Protein <i>NpFBA1</i> Causes Leaf Curling and Reduces Resistance to Black Shank Disease in TobaccoZhongyi Xie0Guo Wen1Yao Yang2Haiyan Wang3Jinying Wang4Chenggong Lei5Qigao Guo6Jiangbo Dang7Guolu Liang8Key Laboratory of Horticulture Science for Southern Mountains Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaKey Laboratory of Horticulture Science for Southern Mountains Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaKey Laboratory of Horticulture Science for Southern Mountains Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaKey Laboratory of Horticulture Science for Southern Mountains Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaKey Laboratory of Horticulture Science for Southern Mountains Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaKey Laboratory of Horticulture Science for Southern Mountains Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaKey Laboratory of Horticulture Science for Southern Mountains Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaKey Laboratory of Horticulture Science for Southern Mountains Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaKey Laboratory of Horticulture Science for Southern Mountains Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaPlant leaf morphology has a great impact on plant drought resistance, ornamental research and leaf yield. In this study, we identified a new gene in <i>Nicotiana plumbaginifolia</i>, <i>NpFBA1</i>, that causes leaf curl. The results show that the <i>NpFBA1</i> protein contains only one unique F-box associated (FBA) domain and does not have an F-box conserved domain. Phylogenetic analysis placed this gene and other Nicotiana FBA genes on a separate branch, and the <i>NpFBA1</i> protein localized to the nucleus and cytoplasm. The expression of <i>NpFBA1</i> was induced by black shank pathogen (<i>Phytophthora parasitica</i> var. <i>nicotianae</i>) infection and treatment with salicylic acid (SA) and methyl jasmonate (MeJA). <i>NpFBA1</i>-overexpressing transgenic lines showed leaf curling and aging during the rosette phase. During the bolting period, the leaves were curly and rounded, and the plants were dwarfed. In addition, <i>NpFBA1</i>-overexpressing lines were more susceptible to disease than wild-type (WT) plants. Further studies revealed that overexpression of <i>NpFBA1</i> significantly downregulated the expression of auxin response factors such as <i>NtARF3</i> and the lignin synthesis genes <i>NtPAL</i>, <i>NtC4H</i>, <i>NtCAD2</i>, and <i>NtCCR1</i> in the leaves. In conclusion, <i>NpFBA1</i> may play a key role in regulating leaf development and the response to pathogen infection.https://www.mdpi.com/2073-4395/11/12/2478<i>Nicotiana plumbaginifolia</i><i>NpFBA1</i>overexpressionleaf curlingsusceptibility |
| spellingShingle | Zhongyi Xie Guo Wen Yao Yang Haiyan Wang Jinying Wang Chenggong Lei Qigao Guo Jiangbo Dang Guolu Liang The FBA Motif-Containing Protein <i>NpFBA1</i> Causes Leaf Curling and Reduces Resistance to Black Shank Disease in Tobacco Agronomy <i>Nicotiana plumbaginifolia</i> <i>NpFBA1</i> overexpression leaf curling susceptibility |
| title | The FBA Motif-Containing Protein <i>NpFBA1</i> Causes Leaf Curling and Reduces Resistance to Black Shank Disease in Tobacco |
| title_full | The FBA Motif-Containing Protein <i>NpFBA1</i> Causes Leaf Curling and Reduces Resistance to Black Shank Disease in Tobacco |
| title_fullStr | The FBA Motif-Containing Protein <i>NpFBA1</i> Causes Leaf Curling and Reduces Resistance to Black Shank Disease in Tobacco |
| title_full_unstemmed | The FBA Motif-Containing Protein <i>NpFBA1</i> Causes Leaf Curling and Reduces Resistance to Black Shank Disease in Tobacco |
| title_short | The FBA Motif-Containing Protein <i>NpFBA1</i> Causes Leaf Curling and Reduces Resistance to Black Shank Disease in Tobacco |
| title_sort | fba motif containing protein i npfba1 i causes leaf curling and reduces resistance to black shank disease in tobacco |
| topic | <i>Nicotiana plumbaginifolia</i> <i>NpFBA1</i> overexpression leaf curling susceptibility |
| url | https://www.mdpi.com/2073-4395/11/12/2478 |
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