Minimal Residual Disease Detection Using Gene Scanning Analysis, Fluorescent Fragment Analysis, and Capillary Electrophoresis for IgH Rearrangement in Adult B-Lineage Acute Lymphoblastic Leukemia: A Cross-Sectional Study
Objective: Minimal residual disease (MRD) is considered the greatest prognostic factor in acute lymphoblastic leukemia(ALL). MRD is a valuable tool for anticipating impending relapse and treatment response assessment. The objective ofthe present study was to investigate whether the detection of IgH...
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Royan Institute (ACECR), Tehran
2023-02-01
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| Series: | Cell Journal |
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| Online Access: | https://www.celljournal.org/article_701270_10d55f2d99dee9d331af491fa317890e.pdf |
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| author | Sepideh Shahkarami Samareh Younesian Shahrbano Rostami Farzad Kompani Davood Bashash Seyed Mousavi Seyed Ghaffari |
| author_facet | Sepideh Shahkarami Samareh Younesian Shahrbano Rostami Farzad Kompani Davood Bashash Seyed Mousavi Seyed Ghaffari |
| author_sort | Sepideh Shahkarami |
| collection | DOAJ |
| description | Objective: Minimal residual disease (MRD) is considered the greatest prognostic factor in acute lymphoblastic leukemia(ALL). MRD is a valuable tool for anticipating impending relapse and treatment response assessment. The objective ofthe present study was to investigate whether the detection of IgH gene rearrangement using polymerase chain reaction(PCR)-based GeneScan analysis could be a complementary method to monitor MRD along with the quantitative realtimePCR (qPCR).Materials and Methods: In this cross-sectional study, we valued the MRD levels, based on the GeneScanning analysis(GSA), and then compared the data with quantitative real-time polymerase chain reaction at different time points inperipheral blood (PB) samples of adult B-lineage ALL patients (n=35). The specific polymerase chain reaction (PCR)primers for IGH gene FR-1 and fluorescence-labeled J-primer were used and analyzed by capillary gel electrophoresison a sequencer. The results of this study were compared with the previously reported MRD results obtained by the IGHrearrangements allele-specific oligonucleotide (ASO) -qPCR methods.Results: The total concordance rate was 86.7%, with a P<0.001. MRD results obtained by GSA and ASO-qPCR methodswere concordant in all diagnostic samples and samples on the 14th and 28th days of induction therapy. The results of these 2.5years’ follow-ups demonstrated a significant correlation between the two techniques (r=0.892, P<0.001).Conclusion: It seems that the PCR-based GeneScan analysis of IGH gene rearrangement detection may be a valuablemolecular technique to distinguish monoclonality from polyclonality. And also, it may be a precise tool to detect theresidual leukemic DNA in the PB follow-up samples of patients. |
| first_indexed | 2024-04-10T07:16:01Z |
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| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-04-10T07:16:01Z |
| publishDate | 2023-02-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-405207f106af4f929872eed85a2a59882023-02-25T09:41:26ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142023-02-01252859110.22074/cellj.2023.557390.1049701270Minimal Residual Disease Detection Using Gene Scanning Analysis, Fluorescent Fragment Analysis, and Capillary Electrophoresis for IgH Rearrangement in Adult B-Lineage Acute Lymphoblastic Leukemia: A Cross-Sectional StudySepideh Shahkarami0Samareh Younesian1Shahrbano Rostami2Farzad Kompani3Davood Bashash4Seyed Mousavi5Seyed Ghaffari6Hematology, Oncology and Stem Cell Transplantation Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, IranHematology, Oncology and Stem Cell Transplantation Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, IranHematology, Oncology and Stem Cell Transplantation Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, IranResearch Center for Immunodeficiencies, Children’s Medical Center, Tehran University of Medical Sciences, Tehran, IranDepartment of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, IranHematology, Oncology and Stem Cell Transplantation Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, IranHematology, Oncology and Stem Cell Transplantation Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, IranObjective: Minimal residual disease (MRD) is considered the greatest prognostic factor in acute lymphoblastic leukemia(ALL). MRD is a valuable tool for anticipating impending relapse and treatment response assessment. The objective ofthe present study was to investigate whether the detection of IgH gene rearrangement using polymerase chain reaction(PCR)-based GeneScan analysis could be a complementary method to monitor MRD along with the quantitative realtimePCR (qPCR).Materials and Methods: In this cross-sectional study, we valued the MRD levels, based on the GeneScanning analysis(GSA), and then compared the data with quantitative real-time polymerase chain reaction at different time points inperipheral blood (PB) samples of adult B-lineage ALL patients (n=35). The specific polymerase chain reaction (PCR)primers for IGH gene FR-1 and fluorescence-labeled J-primer were used and analyzed by capillary gel electrophoresison a sequencer. The results of this study were compared with the previously reported MRD results obtained by the IGHrearrangements allele-specific oligonucleotide (ASO) -qPCR methods.Results: The total concordance rate was 86.7%, with a P<0.001. MRD results obtained by GSA and ASO-qPCR methodswere concordant in all diagnostic samples and samples on the 14th and 28th days of induction therapy. The results of these 2.5years’ follow-ups demonstrated a significant correlation between the two techniques (r=0.892, P<0.001).Conclusion: It seems that the PCR-based GeneScan analysis of IGH gene rearrangement detection may be a valuablemolecular technique to distinguish monoclonality from polyclonality. And also, it may be a precise tool to detect theresidual leukemic DNA in the PB follow-up samples of patients.https://www.celljournal.org/article_701270_10d55f2d99dee9d331af491fa317890e.pdfacute lymphoblastic leukemiacapillary gel electrophoresisimmunoglobulin heavy chaingenescanningminimal residual disease |
| spellingShingle | Sepideh Shahkarami Samareh Younesian Shahrbano Rostami Farzad Kompani Davood Bashash Seyed Mousavi Seyed Ghaffari Minimal Residual Disease Detection Using Gene Scanning Analysis, Fluorescent Fragment Analysis, and Capillary Electrophoresis for IgH Rearrangement in Adult B-Lineage Acute Lymphoblastic Leukemia: A Cross-Sectional Study Cell Journal acute lymphoblastic leukemia capillary gel electrophoresis immunoglobulin heavy chain genescanning minimal residual disease |
| title | Minimal Residual Disease Detection Using Gene Scanning Analysis, Fluorescent Fragment Analysis, and Capillary Electrophoresis for IgH Rearrangement in Adult B-Lineage Acute Lymphoblastic Leukemia: A Cross-Sectional Study |
| title_full | Minimal Residual Disease Detection Using Gene Scanning Analysis, Fluorescent Fragment Analysis, and Capillary Electrophoresis for IgH Rearrangement in Adult B-Lineage Acute Lymphoblastic Leukemia: A Cross-Sectional Study |
| title_fullStr | Minimal Residual Disease Detection Using Gene Scanning Analysis, Fluorescent Fragment Analysis, and Capillary Electrophoresis for IgH Rearrangement in Adult B-Lineage Acute Lymphoblastic Leukemia: A Cross-Sectional Study |
| title_full_unstemmed | Minimal Residual Disease Detection Using Gene Scanning Analysis, Fluorescent Fragment Analysis, and Capillary Electrophoresis for IgH Rearrangement in Adult B-Lineage Acute Lymphoblastic Leukemia: A Cross-Sectional Study |
| title_short | Minimal Residual Disease Detection Using Gene Scanning Analysis, Fluorescent Fragment Analysis, and Capillary Electrophoresis for IgH Rearrangement in Adult B-Lineage Acute Lymphoblastic Leukemia: A Cross-Sectional Study |
| title_sort | minimal residual disease detection using gene scanning analysis fluorescent fragment analysis and capillary electrophoresis for igh rearrangement in adult b lineage acute lymphoblastic leukemia a cross sectional study |
| topic | acute lymphoblastic leukemia capillary gel electrophoresis immunoglobulin heavy chain genescanning minimal residual disease |
| url | https://www.celljournal.org/article_701270_10d55f2d99dee9d331af491fa317890e.pdf |
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