Identification and validation of the reference genes in the echiuran worm Urechis unicinctus based on transcriptome data

Identification and validation of the reference genes in the echiuran worm Urechis unicinctus based on transcriptome data

Abstract Background Real-time quantitative PCR (RT-qPCR) is a crucial and widely used method for gene expression analysis. Selecting suitable reference genes is extremely important for the accuracy of RT-qPCR results. Commonly used reference genes are not always stable in various organisms or under...

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Bibliographic Details
Main Authors: Jiao Chen, Yunjian Wang, Zhi Yang, Danwen Liu, Yao Jin, Xixi Li, Yuhang Deng, Boya Wang, Zhifeng Zhang, Yubin Ma
Format: Article
Language:English
Published: BMC 2023-05-01
Series:BMC Genomics
Subjects:
Reference genes
RT-qPCR
Transcriptome data
Urechis unicinctus
Online Access:https://doi.org/10.1186/s12864-023-09358-6

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