Metabolic and morphological profile in skeletal muscle of healthy boys and girls
Abstract It was hypothesized that the typical adult pattern of higher glycolytic capacity in skeletal muscle of males compared to females is not observed in children and that fiber cross‐sectional area (CSA) is a determinant of glycolytic capacity in children. Biopsies were performed in vastus later...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-08-01
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| Series: | Physiological Reports |
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| Online Access: | https://doi.org/10.14814/phy2.15414 |
| _version_ | 1828744198581387264 |
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| author | Mona Esbjörnsson Barbara Norman Monica Dahlström Jan Gierup Eva Jansson |
| author_facet | Mona Esbjörnsson Barbara Norman Monica Dahlström Jan Gierup Eva Jansson |
| author_sort | Mona Esbjörnsson |
| collection | DOAJ |
| description | Abstract It was hypothesized that the typical adult pattern of higher glycolytic capacity in skeletal muscle of males compared to females is not observed in children and that fiber cross‐sectional area (CSA) is a determinant of glycolytic capacity in children. Biopsies were performed in vastus lateralis in 9–12 years‐old healthy boys and girls (N = 27). Fiber types were classified by myofibrillar ATPase staining and CSA was measured using planimetry. Citrate synthase (CS) and lactate dehydrogenase (LD) were analyzed using fluorometric and spectrophotometric methods. There was no significant difference between boys and girls in CS activity (0.45 ± 0.1 μkat g−1 dry muscle in boys and 0.42 ± 0.1 in girls) or LD activity (24 ± 6 μkat g−1 dry muscle in boys and 25 ± 7 in girls). CSA did not differ between boys and girls. CS was inversely related to type I CSA (r = −0.62, p < 0.001) and LD was directly related to type IIA (r = 0.63, p < 0.001) and type IIB CSA (r = 0.72, p < 0.001). CSA was a significant determinant of CS and LD, even after adjusting for sex and relative fiber type area in multiple regression analysis. This suggests that the typical adult pattern of higher muscle glycolytic capacity in males than in females, as estimated by LD activity, was not observed in children. Sex‐specific patterns in glycolytic capacity thus appear to develop during the transition from childhood to adulthood. In addition, fiber CSA was a strong determinant of both muscle glycolytic and oxidative capacity in children, regardless of sex. |
| first_indexed | 2024-04-13T01:52:41Z |
| format | Article |
| id | doaj.art-40528673e27342cd8b9d90711ed3fd91 |
| institution | Directory Open Access Journal |
| issn | 2051-817X |
| language | English |
| last_indexed | 2024-04-13T01:52:41Z |
| publishDate | 2022-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Physiological Reports |
| spelling | doaj.art-40528673e27342cd8b9d90711ed3fd912022-12-22T03:07:51ZengWileyPhysiological Reports2051-817X2022-08-011016n/an/a10.14814/phy2.15414Metabolic and morphological profile in skeletal muscle of healthy boys and girlsMona Esbjörnsson0Barbara Norman1Monica Dahlström2Jan Gierup3Eva Jansson4Division of Clinical Physiology, Department of Laboratory Medicine Karolinska Institutet Stockholm SwedenDivision of Clinical Physiology, Department of Laboratory Medicine Karolinska Institutet Stockholm SwedenSollentuna SwedenLidingö SwedenDivision of Clinical Physiology, Department of Laboratory Medicine Karolinska Institutet Stockholm SwedenAbstract It was hypothesized that the typical adult pattern of higher glycolytic capacity in skeletal muscle of males compared to females is not observed in children and that fiber cross‐sectional area (CSA) is a determinant of glycolytic capacity in children. Biopsies were performed in vastus lateralis in 9–12 years‐old healthy boys and girls (N = 27). Fiber types were classified by myofibrillar ATPase staining and CSA was measured using planimetry. Citrate synthase (CS) and lactate dehydrogenase (LD) were analyzed using fluorometric and spectrophotometric methods. There was no significant difference between boys and girls in CS activity (0.45 ± 0.1 μkat g−1 dry muscle in boys and 0.42 ± 0.1 in girls) or LD activity (24 ± 6 μkat g−1 dry muscle in boys and 25 ± 7 in girls). CSA did not differ between boys and girls. CS was inversely related to type I CSA (r = −0.62, p < 0.001) and LD was directly related to type IIA (r = 0.63, p < 0.001) and type IIB CSA (r = 0.72, p < 0.001). CSA was a significant determinant of CS and LD, even after adjusting for sex and relative fiber type area in multiple regression analysis. This suggests that the typical adult pattern of higher muscle glycolytic capacity in males than in females, as estimated by LD activity, was not observed in children. Sex‐specific patterns in glycolytic capacity thus appear to develop during the transition from childhood to adulthood. In addition, fiber CSA was a strong determinant of both muscle glycolytic and oxidative capacity in children, regardless of sex.https://doi.org/10.14814/phy2.15414agechildrencross‐sectional areafiber typesmetabolic enzymessex |
| spellingShingle | Mona Esbjörnsson Barbara Norman Monica Dahlström Jan Gierup Eva Jansson Metabolic and morphological profile in skeletal muscle of healthy boys and girls Physiological Reports age children cross‐sectional area fiber types metabolic enzymes sex |
| title | Metabolic and morphological profile in skeletal muscle of healthy boys and girls |
| title_full | Metabolic and morphological profile in skeletal muscle of healthy boys and girls |
| title_fullStr | Metabolic and morphological profile in skeletal muscle of healthy boys and girls |
| title_full_unstemmed | Metabolic and morphological profile in skeletal muscle of healthy boys and girls |
| title_short | Metabolic and morphological profile in skeletal muscle of healthy boys and girls |
| title_sort | metabolic and morphological profile in skeletal muscle of healthy boys and girls |
| topic | age children cross‐sectional area fiber types metabolic enzymes sex |
| url | https://doi.org/10.14814/phy2.15414 |
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