Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes
For several decades the MCM2-7 proteins, the core of the DNA replicative helicase, eluded detection at DNA replication sites. Here, the authors solve this conundrum by gene editing, which enables visualization of replication dynamics in living cells.
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-10-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-33887-5 |
| _version_ | 1811238708688453632 |
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| author | Hana Polasek-Sedlackova Thomas C. R. Miller Jana Krejci Maj-Britt Rask Jiri Lukas |
| author_facet | Hana Polasek-Sedlackova Thomas C. R. Miller Jana Krejci Maj-Britt Rask Jiri Lukas |
| author_sort | Hana Polasek-Sedlackova |
| collection | DOAJ |
| description | For several decades the MCM2-7 proteins, the core of the DNA replicative helicase, eluded detection at DNA replication sites. Here, the authors solve this conundrum by gene editing, which enables visualization of replication dynamics in living cells. |
| first_indexed | 2024-04-12T12:46:58Z |
| format | Article |
| id | doaj.art-40546856371644f190ce151cd486b1df |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-12T12:46:58Z |
| publishDate | 2022-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-40546856371644f190ce151cd486b1df2022-12-22T03:32:36ZengNature PortfolioNature Communications2041-17232022-10-0113111110.1038/s41467-022-33887-5Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomesHana Polasek-Sedlackova0Thomas C. R. Miller1Jana Krejci2Maj-Britt Rask3Jiri Lukas4Protein Signaling Program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of CopenhagenCenter for Chromosome Stability, Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of CopenhagenDepartment of Cell Biology and Epigenetics, Institute of Biophysics, Czech Academy of SciencesProtein Signaling Program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of CopenhagenProtein Signaling Program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of CopenhagenFor several decades the MCM2-7 proteins, the core of the DNA replicative helicase, eluded detection at DNA replication sites. Here, the authors solve this conundrum by gene editing, which enables visualization of replication dynamics in living cells.https://doi.org/10.1038/s41467-022-33887-5 |
| spellingShingle | Hana Polasek-Sedlackova Thomas C. R. Miller Jana Krejci Maj-Britt Rask Jiri Lukas Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes Nature Communications |
| title | Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes |
| title_full | Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes |
| title_fullStr | Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes |
| title_full_unstemmed | Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes |
| title_short | Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes |
| title_sort | solving the mcm paradox by visualizing the scaffold of cmg helicase at active replisomes |
| url | https://doi.org/10.1038/s41467-022-33887-5 |
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