Potential of the endogenous and artificially inserted CRISPR-Cas system for controlling virulence and antimicrobial resistance of food pathogens
Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated sequences (Cas) are prokaryotic defenses against viruses and foreign nucleic acids found in many bacterial and archaeal genomes. The discovery that CRISPR spacers are often identical to sequence fragments of mob...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-10-01
|
| Series: | Food Chemistry Advances |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772753X23000497 |
| _version_ | 1797796040034746368 |
|---|---|
| author | Ramila Cristiane Rodrigues Thaysa Leite Tagliaferri Tiago Antônio de Oliveira Mendes |
| author_facet | Ramila Cristiane Rodrigues Thaysa Leite Tagliaferri Tiago Antônio de Oliveira Mendes |
| author_sort | Ramila Cristiane Rodrigues |
| collection | DOAJ |
| description | Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated sequences (Cas) are prokaryotic defenses against viruses and foreign nucleic acids found in many bacterial and archaeal genomes. The discovery that CRISPR spacers are often identical to sequence fragments of mobile genetic elements was a major breakthrough that eventually led to the elucidation of CRISPR-Cas as an adaptive immunity system. The ability of the CRISPR-Cas systems to perform sequence-specific DNA cleavage evidenced its potential for gene deletion, insertion, or regulation. This allows the food microbiota to be easily genetically modulated, including virulence or resistance gene editing from pathogens which could lead to more safe and high-quality products. This review provides insights into the CRISPR-Cas systems, followed by the understanding of the synergistic or antagonistic relationship of resistance and virulence determinants in foodborne pathogens in connection to their intrinsic CRISPR system. By employing specific examples and recently reported studies this review also widens the discussion of the CRISPR-Cas use for controlling food pathogens by editing genes associated with virulence modulation and reversal of antimicrobial resistance. |
| first_indexed | 2024-03-13T03:27:08Z |
| format | Article |
| id | doaj.art-4054a35fc5544630b208b20b1b7f23c8 |
| institution | Directory Open Access Journal |
| issn | 2772-753X |
| language | English |
| last_indexed | 2024-03-13T03:27:08Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry Advances |
| spelling | doaj.art-4054a35fc5544630b208b20b1b7f23c82023-06-25T04:44:42ZengElsevierFood Chemistry Advances2772-753X2023-10-012100229Potential of the endogenous and artificially inserted CRISPR-Cas system for controlling virulence and antimicrobial resistance of food pathogensRamila Cristiane Rodrigues0Thaysa Leite Tagliaferri1Tiago Antônio de Oliveira Mendes2Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Viçosa, Brazil; Corresponding authors.Institute of Medical Microbiology, RWTH Aachen University Hospital, Aachen, GermanyDepartment of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Viçosa, Brazil; Corresponding authors.Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated sequences (Cas) are prokaryotic defenses against viruses and foreign nucleic acids found in many bacterial and archaeal genomes. The discovery that CRISPR spacers are often identical to sequence fragments of mobile genetic elements was a major breakthrough that eventually led to the elucidation of CRISPR-Cas as an adaptive immunity system. The ability of the CRISPR-Cas systems to perform sequence-specific DNA cleavage evidenced its potential for gene deletion, insertion, or regulation. This allows the food microbiota to be easily genetically modulated, including virulence or resistance gene editing from pathogens which could lead to more safe and high-quality products. This review provides insights into the CRISPR-Cas systems, followed by the understanding of the synergistic or antagonistic relationship of resistance and virulence determinants in foodborne pathogens in connection to their intrinsic CRISPR system. By employing specific examples and recently reported studies this review also widens the discussion of the CRISPR-Cas use for controlling food pathogens by editing genes associated with virulence modulation and reversal of antimicrobial resistance.http://www.sciencedirect.com/science/article/pii/S2772753X23000497CRISPR-Cas systemsAntibiotic-resistant bacteriaVirulenceGenome editingFood pathogens |
| spellingShingle | Ramila Cristiane Rodrigues Thaysa Leite Tagliaferri Tiago Antônio de Oliveira Mendes Potential of the endogenous and artificially inserted CRISPR-Cas system for controlling virulence and antimicrobial resistance of food pathogens Food Chemistry Advances CRISPR-Cas systems Antibiotic-resistant bacteria Virulence Genome editing Food pathogens |
| title | Potential of the endogenous and artificially inserted CRISPR-Cas system for controlling virulence and antimicrobial resistance of food pathogens |
| title_full | Potential of the endogenous and artificially inserted CRISPR-Cas system for controlling virulence and antimicrobial resistance of food pathogens |
| title_fullStr | Potential of the endogenous and artificially inserted CRISPR-Cas system for controlling virulence and antimicrobial resistance of food pathogens |
| title_full_unstemmed | Potential of the endogenous and artificially inserted CRISPR-Cas system for controlling virulence and antimicrobial resistance of food pathogens |
| title_short | Potential of the endogenous and artificially inserted CRISPR-Cas system for controlling virulence and antimicrobial resistance of food pathogens |
| title_sort | potential of the endogenous and artificially inserted crispr cas system for controlling virulence and antimicrobial resistance of food pathogens |
| topic | CRISPR-Cas systems Antibiotic-resistant bacteria Virulence Genome editing Food pathogens |
| url | http://www.sciencedirect.com/science/article/pii/S2772753X23000497 |
| work_keys_str_mv | AT ramilacristianerodrigues potentialoftheendogenousandartificiallyinsertedcrisprcassystemforcontrollingvirulenceandantimicrobialresistanceoffoodpathogens AT thaysaleitetagliaferri potentialoftheendogenousandartificiallyinsertedcrisprcassystemforcontrollingvirulenceandantimicrobialresistanceoffoodpathogens AT tiagoantoniodeoliveiramendes potentialoftheendogenousandartificiallyinsertedcrisprcassystemforcontrollingvirulenceandantimicrobialresistanceoffoodpathogens |