Effect of different factors on micropropagation of Abarkooh 4000-year-old horizontal cypress (Cupressus sempervirens L. var. horizontalis (Mill.) Gord.)
This study was conducted to protect the genetic properties and propagation of 4000-year-old Abarkooh horizontal cypress (Cupressus semprvirens L. var. horizontalis (Mill.) Gord.) by tissue culture technique. In the first experiment, the effect of preparation time of explant (four seasons), medium cu...
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Research Institute of Forests and Rangelands of Iran
2018-06-01
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| Series: | تحقیقات جنگل و صنوبر ایران |
| Subjects: | |
| Online Access: | http://ijfpr.areeo.ac.ir/article_116751_67d62a38ed8ec05855e471affa3bfdda.pdf |
| _version_ | 1818247542398255104 |
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| author | Mahdie Khamushi Maryam Dehestani-Ardakani Kazem Kamali Aliabad Jalal Gholamnejad |
| author_facet | Mahdie Khamushi Maryam Dehestani-Ardakani Kazem Kamali Aliabad Jalal Gholamnejad |
| author_sort | Mahdie Khamushi |
| collection | DOAJ |
| description | This study was conducted to protect the genetic properties and propagation of 4000-year-old Abarkooh horizontal cypress (Cupressus semprvirens L. var. horizontalis (Mill.) Gord.) by tissue culture technique. In the first experiment, the effect of preparation time of explant (four seasons), medium culture (MS and WPM) and concentrations of BA (0, 0.1 and 1 mg/l) on proliferation of horizontal cypress explants was investigated. In second and third experiments the effect of culture media (MS and WPM), concentrations of BA (0, 0.1, 0.5 and 1 mg/l) was studied alone or in combination with 0.01 mg/l IBA and type of explant (primary or secondary shoots) in a factorial experiment and completely randomized design with three replications. In first experiment, the maximum shoots number was obtained from spring and summer explants that were cultured in MS and WPM culture media, respectively. In second experiment, the maximum number and length of shoots was obtained in WPM culture medium and secondary shoots. The highest length of shoots was found in first shoots cultured in WPM medium containing 0.1 and 0.5 mg/l BA, followed by primary shoots cultured in MS medium culture without BA. In the third experiment, the maximum number of shoots (3) was observed in secondary shoots by using WPM medium containing 0 mg/l BA + 0.01 mg/l IBA. Conclusively the best time of preparing explant, type of culture media and explant were found to be spring, WPM medium and secondary shoots, respectively. |
| first_indexed | 2024-12-12T15:06:22Z |
| format | Article |
| id | doaj.art-40560580ff3543108d848b63d5422b8d |
| institution | Directory Open Access Journal |
| issn | 1735-0883 2383-1146 |
| language | fas |
| last_indexed | 2024-12-12T15:06:22Z |
| publishDate | 2018-06-01 |
| publisher | Research Institute of Forests and Rangelands of Iran |
| record_format | Article |
| series | تحقیقات جنگل و صنوبر ایران |
| spelling | doaj.art-40560580ff3543108d848b63d5422b8d2022-12-22T00:20:43ZfasResearch Institute of Forests and Rangelands of Iranتحقیقات جنگل و صنوبر ایران1735-08832383-11462018-06-0126222823910.22092/ijfpr.2018.116751116751Effect of different factors on micropropagation of Abarkooh 4000-year-old horizontal cypress (Cupressus sempervirens L. var. horizontalis (Mill.) Gord.)Mahdie Khamushi0Maryam Dehestani-Ardakani1Kazem Kamali Aliabad2Jalal Gholamnejad3M.Sc. Student, Department of Horticultural Science, Faculty of Agriculture & Natural Resources, Ardakan University, Ardakan, IranAssistant Prof., Department of Horticultural Science, Faculty of Agriculture & Natural Resources, Ardakan University, Ardakan, IranAssistant Prof., Department of Soil Science, Faculty of Natural Resources, Yazd University, Yazd, IranAssistant Prof., Department of Horticultural Science, Faculty of Agriculture & Natural Resources, Ardakan University, Ardakan, IranThis study was conducted to protect the genetic properties and propagation of 4000-year-old Abarkooh horizontal cypress (Cupressus semprvirens L. var. horizontalis (Mill.) Gord.) by tissue culture technique. In the first experiment, the effect of preparation time of explant (four seasons), medium culture (MS and WPM) and concentrations of BA (0, 0.1 and 1 mg/l) on proliferation of horizontal cypress explants was investigated. In second and third experiments the effect of culture media (MS and WPM), concentrations of BA (0, 0.1, 0.5 and 1 mg/l) was studied alone or in combination with 0.01 mg/l IBA and type of explant (primary or secondary shoots) in a factorial experiment and completely randomized design with three replications. In first experiment, the maximum shoots number was obtained from spring and summer explants that were cultured in MS and WPM culture media, respectively. In second experiment, the maximum number and length of shoots was obtained in WPM culture medium and secondary shoots. The highest length of shoots was found in first shoots cultured in WPM medium containing 0.1 and 0.5 mg/l BA, followed by primary shoots cultured in MS medium culture without BA. In the third experiment, the maximum number of shoots (3) was observed in secondary shoots by using WPM medium containing 0 mg/l BA + 0.01 mg/l IBA. Conclusively the best time of preparing explant, type of culture media and explant were found to be spring, WPM medium and secondary shoots, respectively.http://ijfpr.areeo.ac.ir/article_116751_67d62a38ed8ec05855e471affa3bfdda.pdfBenzyl adeninIndolebutyric acidmedium cultureshoot |
| spellingShingle | Mahdie Khamushi Maryam Dehestani-Ardakani Kazem Kamali Aliabad Jalal Gholamnejad Effect of different factors on micropropagation of Abarkooh 4000-year-old horizontal cypress (Cupressus sempervirens L. var. horizontalis (Mill.) Gord.) تحقیقات جنگل و صنوبر ایران Benzyl adenin Indolebutyric acid medium culture shoot |
| title | Effect of different factors on micropropagation of Abarkooh 4000-year-old horizontal cypress (Cupressus sempervirens L. var. horizontalis (Mill.) Gord.) |
| title_full | Effect of different factors on micropropagation of Abarkooh 4000-year-old horizontal cypress (Cupressus sempervirens L. var. horizontalis (Mill.) Gord.) |
| title_fullStr | Effect of different factors on micropropagation of Abarkooh 4000-year-old horizontal cypress (Cupressus sempervirens L. var. horizontalis (Mill.) Gord.) |
| title_full_unstemmed | Effect of different factors on micropropagation of Abarkooh 4000-year-old horizontal cypress (Cupressus sempervirens L. var. horizontalis (Mill.) Gord.) |
| title_short | Effect of different factors on micropropagation of Abarkooh 4000-year-old horizontal cypress (Cupressus sempervirens L. var. horizontalis (Mill.) Gord.) |
| title_sort | effect of different factors on micropropagation of abarkooh 4000 year old horizontal cypress cupressus sempervirens l var horizontalis mill gord |
| topic | Benzyl adenin Indolebutyric acid medium culture shoot |
| url | http://ijfpr.areeo.ac.ir/article_116751_67d62a38ed8ec05855e471affa3bfdda.pdf |
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