LEPR polymorphisms and haplotypes in Mexican patients with colorectal cancer
Introduction: Obesity and colorectal cancer could be linked by adipocytokines, which are proteins associated with cell proliferation. High levels of the adipocytokine leptin promote the development of colorectal cancer through its receptor. Objective: To determine the association between c.326A>G...
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| Language: | English |
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Instituto Nacional de Salud
2019-03-01
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| Series: | Biomédica: revista del Instituto Nacional de Salud |
| Subjects: | |
| Online Access: | https://www.revistabiomedica.org/index.php/biomedica/article/view/4091 |
| _version_ | 1811343016196046848 |
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| author | Miriam Partida Melva Gutiérrez María de la Luz Ayala Nelly Margarita Macías Carlos Rogelio Alvizo Jorge Peregrina |
| author_facet | Miriam Partida Melva Gutiérrez María de la Luz Ayala Nelly Margarita Macías Carlos Rogelio Alvizo Jorge Peregrina |
| author_sort | Miriam Partida |
| collection | DOAJ |
| description | Introduction: Obesity and colorectal cancer could be linked by adipocytokines, which are proteins associated with cell proliferation. High levels of the adipocytokine leptin promote the development of colorectal cancer through its receptor.
Objective: To determine the association between c.326A>G and c.668A>G LEPR gene polymorphisms and colorectal cancer.
Materials and methods: DNA was extracted from the peripheral blood of 147 patients with sporadic colorectal cancer and 134 healthy people. Genotypes were obtained by PCRRFLP and the association was determined by the odds ratio (OR) test using the SPSS™, version 10.0, program. Haplotype frequencies and linkage disequilibrium were estimated by the Arlequin, version 3.5, software.
Results: Both polymorphisms were in Hardy-Weinberg equilibrium. Only the c.326A>G heterozygous genotype revealed an increased risk for colorectal cancer development (OR=1.81, 95% CI=1.04-3.16, p=0.04). The AG haplotype showed a significant association with colorectal cancer (OR=0.58, 95% CI=0.35-0.96, p<0.03). Linkage disequilibrium between the variants was only evident for the patients group (r2=0.36).
Conclusion: Our results suggest that AG individuals heterozygous for the c.326A>G LEPR variant have a higher risk of colorectal cancer development whereas the AG haplotype (c.326A/c.668G) has a protective effect in the Mexican population. |
| first_indexed | 2024-04-13T19:22:19Z |
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| id | doaj.art-405655b08b4540a79c2207a282ddb7fc |
| institution | Directory Open Access Journal |
| issn | 0120-4157 0120-4157 |
| language | English |
| last_indexed | 2024-04-13T19:22:19Z |
| publishDate | 2019-03-01 |
| publisher | Instituto Nacional de Salud |
| record_format | Article |
| series | Biomédica: revista del Instituto Nacional de Salud |
| spelling | doaj.art-405655b08b4540a79c2207a282ddb7fc2022-12-22T02:33:29ZengInstituto Nacional de SaludBiomédica: revista del Instituto Nacional de Salud0120-41570120-41572019-03-0139120521110.7705/biomedica.v39i1.40914091LEPR polymorphisms and haplotypes in Mexican patients with colorectal cancerMiriam Partida0Melva Gutiérrez1María de la Luz Ayala2Nelly Margarita Macías3Carlos Rogelio Alvizo4Jorge Peregrina5Departamento de Ciencias Médicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Puerto Vallarta, Jalisco, MéxicoDoctorado en Genética Humana, Instituto de Genética Humana, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, México; Departamento de Clínicas, Centro Universitario de los Altos, Universidad de Guadalajara, Tepatitlán de Morelos, Jalisco, MéxicoDoctorado en Genética Humana, Instituto de Genética Humana, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, MéxicoLaboratorio de Genética Humana, Centro Universitario del Sur, Universidad de Guadalajara, Ciudad Guzmán, Jalisco, MéxicoDoctorado en Genética Humana, Instituto de Genética Humana, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, MéxicoInstituto de Fisiología Celular, Departamento de Biología Celular y Molecular, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Zapopán, Jalisco, México; Hospital Civil de Guadalajara Fray Antonio Alcalde, Guadalajara, Jalisco, MéxicoIntroduction: Obesity and colorectal cancer could be linked by adipocytokines, which are proteins associated with cell proliferation. High levels of the adipocytokine leptin promote the development of colorectal cancer through its receptor. Objective: To determine the association between c.326A>G and c.668A>G LEPR gene polymorphisms and colorectal cancer. Materials and methods: DNA was extracted from the peripheral blood of 147 patients with sporadic colorectal cancer and 134 healthy people. Genotypes were obtained by PCRRFLP and the association was determined by the odds ratio (OR) test using the SPSS™, version 10.0, program. Haplotype frequencies and linkage disequilibrium were estimated by the Arlequin, version 3.5, software. Results: Both polymorphisms were in Hardy-Weinberg equilibrium. Only the c.326A>G heterozygous genotype revealed an increased risk for colorectal cancer development (OR=1.81, 95% CI=1.04-3.16, p=0.04). The AG haplotype showed a significant association with colorectal cancer (OR=0.58, 95% CI=0.35-0.96, p<0.03). Linkage disequilibrium between the variants was only evident for the patients group (r2=0.36). Conclusion: Our results suggest that AG individuals heterozygous for the c.326A>G LEPR variant have a higher risk of colorectal cancer development whereas the AG haplotype (c.326A/c.668G) has a protective effect in the Mexican population.https://www.revistabiomedica.org/index.php/biomedica/article/view/4091Opportunistic infections/epidemiology; HIV infections; socioeconomic factors; health status disparities; health systems |
| spellingShingle | Miriam Partida Melva Gutiérrez María de la Luz Ayala Nelly Margarita Macías Carlos Rogelio Alvizo Jorge Peregrina LEPR polymorphisms and haplotypes in Mexican patients with colorectal cancer Biomédica: revista del Instituto Nacional de Salud Opportunistic infections/epidemiology; HIV infections; socioeconomic factors; health status disparities; health systems |
| title | LEPR polymorphisms and haplotypes in Mexican patients with colorectal cancer |
| title_full | LEPR polymorphisms and haplotypes in Mexican patients with colorectal cancer |
| title_fullStr | LEPR polymorphisms and haplotypes in Mexican patients with colorectal cancer |
| title_full_unstemmed | LEPR polymorphisms and haplotypes in Mexican patients with colorectal cancer |
| title_short | LEPR polymorphisms and haplotypes in Mexican patients with colorectal cancer |
| title_sort | lepr polymorphisms and haplotypes in mexican patients with colorectal cancer |
| topic | Opportunistic infections/epidemiology; HIV infections; socioeconomic factors; health status disparities; health systems |
| url | https://www.revistabiomedica.org/index.php/biomedica/article/view/4091 |
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