A high-throughput screening and computation platform for identifying synthetic promoters with enhanced cell-state specificity (SPECS)
Synthetic promoters can be superior to native ones but the design is challenging without knowledge of gene regulation. Here the authors develop a pipeline that allows for screening a synthetic promoter library to identify high performance promoters in potentially any given cell state of interest.
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2019-06-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-019-10912-8 |
| _version_ | 1818405246644256768 |
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| author | Ming-Ru Wu Lior Nissim Doron Stupp Erez Pery Adina Binder-Nissim Karen Weisinger Casper Enghuus Sebastian R. Palacios Melissa Humphrey Zhizhuo Zhang Eva Maria Novoa Manolis Kellis Ron Weiss Samuel D. Rabkin Yuval Tabach Timothy K. Lu |
| author_facet | Ming-Ru Wu Lior Nissim Doron Stupp Erez Pery Adina Binder-Nissim Karen Weisinger Casper Enghuus Sebastian R. Palacios Melissa Humphrey Zhizhuo Zhang Eva Maria Novoa Manolis Kellis Ron Weiss Samuel D. Rabkin Yuval Tabach Timothy K. Lu |
| author_sort | Ming-Ru Wu |
| collection | DOAJ |
| description | Synthetic promoters can be superior to native ones but the design is challenging without knowledge of gene regulation. Here the authors develop a pipeline that allows for screening a synthetic promoter library to identify high performance promoters in potentially any given cell state of interest. |
| first_indexed | 2024-12-14T08:53:00Z |
| format | Article |
| id | doaj.art-4058b7f205214aa99e7ffdf21a5903d9 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-14T08:53:00Z |
| publishDate | 2019-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-4058b7f205214aa99e7ffdf21a5903d92022-12-21T23:09:00ZengNature PortfolioNature Communications2041-17232019-06-0110111010.1038/s41467-019-10912-8A high-throughput screening and computation platform for identifying synthetic promoters with enhanced cell-state specificity (SPECS)Ming-Ru Wu0Lior Nissim1Doron Stupp2Erez Pery3Adina Binder-Nissim4Karen Weisinger5Casper Enghuus6Sebastian R. Palacios7Melissa Humphrey8Zhizhuo Zhang9Eva Maria Novoa10Manolis Kellis11Ron Weiss12Samuel D. Rabkin13Yuval Tabach14Timothy K. Lu15Synthetic Biology Group, Research Laboratory of Electronics, Massachusetts Institute of TechnologyDepartment of Biochemistry and Molecular Biology, The Institute for Medical Research Israel-Canada, Hadassah Medical School, The Hebrew University of JerusalemDepartment of Developmental Biology and Cancer Research, The Institute for Medical Research Israel-Canada, Hadassah Medical School, The Hebrew University of JerusalemSynthetic Biology Group, Research Laboratory of Electronics, Massachusetts Institute of TechnologySynthetic Biology Group, Research Laboratory of Electronics, Massachusetts Institute of TechnologySynthetic Biology Group, Research Laboratory of Electronics, Massachusetts Institute of TechnologyDepartment of Biological Engineering, Massachusetts Institute of TechnologySynthetic Biology Center, Massachusetts Institute of TechnologyBrain Tumor Research Center, Department of Neurosurgery, Massachusetts General HospitalComputer Science and Artificial Intelligence Laboratory, Massachusetts Institute of TechnologyComputer Science and Artificial Intelligence Laboratory, Massachusetts Institute of TechnologyComputer Science and Artificial Intelligence Laboratory, Massachusetts Institute of TechnologyDepartment of Biological Engineering, Massachusetts Institute of TechnologyBrain Tumor Research Center, Department of Neurosurgery, Massachusetts General HospitalDepartment of Developmental Biology and Cancer Research, The Institute for Medical Research Israel-Canada, Hadassah Medical School, The Hebrew University of JerusalemSynthetic Biology Group, Research Laboratory of Electronics, Massachusetts Institute of TechnologySynthetic promoters can be superior to native ones but the design is challenging without knowledge of gene regulation. Here the authors develop a pipeline that allows for screening a synthetic promoter library to identify high performance promoters in potentially any given cell state of interest.https://doi.org/10.1038/s41467-019-10912-8 |
| spellingShingle | Ming-Ru Wu Lior Nissim Doron Stupp Erez Pery Adina Binder-Nissim Karen Weisinger Casper Enghuus Sebastian R. Palacios Melissa Humphrey Zhizhuo Zhang Eva Maria Novoa Manolis Kellis Ron Weiss Samuel D. Rabkin Yuval Tabach Timothy K. Lu A high-throughput screening and computation platform for identifying synthetic promoters with enhanced cell-state specificity (SPECS) Nature Communications |
| title | A high-throughput screening and computation platform for identifying synthetic promoters with enhanced cell-state specificity (SPECS) |
| title_full | A high-throughput screening and computation platform for identifying synthetic promoters with enhanced cell-state specificity (SPECS) |
| title_fullStr | A high-throughput screening and computation platform for identifying synthetic promoters with enhanced cell-state specificity (SPECS) |
| title_full_unstemmed | A high-throughput screening and computation platform for identifying synthetic promoters with enhanced cell-state specificity (SPECS) |
| title_short | A high-throughput screening and computation platform for identifying synthetic promoters with enhanced cell-state specificity (SPECS) |
| title_sort | high throughput screening and computation platform for identifying synthetic promoters with enhanced cell state specificity specs |
| url | https://doi.org/10.1038/s41467-019-10912-8 |
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