A clinically validated human saliva metatranscriptomic test for global systems biology studies
The authors report here the development of a high-throughput, automated, inexpensive and clinically validated saliva metatranscriptome test that requires less than 100 μl of saliva. RNA is preserved at the time of sample collection, allowing for ambient-temperature transportation and storage for up...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Future Science Ltd
2023-01-01
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| Series: | BioTechniques |
| Subjects: | |
| Online Access: | https://www.future-science.com/doi/10.2144/btn-2022-0104 |
| _version_ | 1811176819928334336 |
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| author | Ryan Toma Ying Cai Oyetunji Ogundijo Lan Hu Stephanie Gline Diana Demusaj Nathan Duval Pedro Torres Francine Camacho Guruduth Banavar Momchilo Vuyisich |
| author_facet | Ryan Toma Ying Cai Oyetunji Ogundijo Lan Hu Stephanie Gline Diana Demusaj Nathan Duval Pedro Torres Francine Camacho Guruduth Banavar Momchilo Vuyisich |
| author_sort | Ryan Toma |
| collection | DOAJ |
| description | The authors report here the development of a high-throughput, automated, inexpensive and clinically validated saliva metatranscriptome test that requires less than 100 μl of saliva. RNA is preserved at the time of sample collection, allowing for ambient-temperature transportation and storage for up to 28 days. Critically, the RNA preservative is also able to inactivate pathogenic microorganisms, rendering the samples noninfectious and allowing for safe and easy shipping. Given the unique set of convenience, low cost, safety and technical performance, this saliva metatranscriptomic test can be integrated into longitudinal, global-scale systems biology studies that will lead to an accelerated development of precision medicine, diagnostic and therapeutic tools. |
| first_indexed | 2024-04-10T19:59:06Z |
| format | Article |
| id | doaj.art-405c67a6dc9941868b35e374d564330b |
| institution | Directory Open Access Journal |
| issn | 0736-6205 1940-9818 |
| language | English |
| last_indexed | 2024-04-10T19:59:06Z |
| publishDate | 2023-01-01 |
| publisher | Future Science Ltd |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj.art-405c67a6dc9941868b35e374d564330b2023-01-27T12:13:06ZengFuture Science LtdBioTechniques0736-62051940-98182023-01-01741314410.2144/btn-2022-0104A clinically validated human saliva metatranscriptomic test for global systems biology studiesRyan Toma0Ying Cai1Oyetunji Ogundijo2Lan Hu3Stephanie Gline4Diana Demusaj5Nathan Duval6Pedro Torres7Francine Camacho8Guruduth Banavar9Momchilo Vuyisich101Viome, Inc. Viome Life Sciences, Bothell/Bellevue WA 98011/98004. Viome Bioinformatics, New York, NY 10018, USA1Viome, Inc. Viome Life Sciences, Bothell/Bellevue WA 98011/98004. Viome Bioinformatics, New York, NY 10018, USA1Viome, Inc. Viome Life Sciences, Bothell/Bellevue WA 98011/98004. Viome Bioinformatics, New York, NY 10018, USA1Viome, Inc. Viome Life Sciences, Bothell/Bellevue WA 98011/98004. Viome Bioinformatics, New York, NY 10018, USA1Viome, Inc. Viome Life Sciences, Bothell/Bellevue WA 98011/98004. Viome Bioinformatics, New York, NY 10018, USA1Viome, Inc. Viome Life Sciences, Bothell/Bellevue WA 98011/98004. Viome Bioinformatics, New York, NY 10018, USA1Viome, Inc. Viome Life Sciences, Bothell/Bellevue WA 98011/98004. Viome Bioinformatics, New York, NY 10018, USA1Viome, Inc. Viome Life Sciences, Bothell/Bellevue WA 98011/98004. Viome Bioinformatics, New York, NY 10018, USA1Viome, Inc. Viome Life Sciences, Bothell/Bellevue WA 98011/98004. Viome Bioinformatics, New York, NY 10018, USA1Viome, Inc. Viome Life Sciences, Bothell/Bellevue WA 98011/98004. Viome Bioinformatics, New York, NY 10018, USA1Viome, Inc. Viome Life Sciences, Bothell/Bellevue WA 98011/98004. Viome Bioinformatics, New York, NY 10018, USAThe authors report here the development of a high-throughput, automated, inexpensive and clinically validated saliva metatranscriptome test that requires less than 100 μl of saliva. RNA is preserved at the time of sample collection, allowing for ambient-temperature transportation and storage for up to 28 days. Critically, the RNA preservative is also able to inactivate pathogenic microorganisms, rendering the samples noninfectious and allowing for safe and easy shipping. Given the unique set of convenience, low cost, safety and technical performance, this saliva metatranscriptomic test can be integrated into longitudinal, global-scale systems biology studies that will lead to an accelerated development of precision medicine, diagnostic and therapeutic tools.https://www.future-science.com/doi/10.2144/btn-2022-0104clinically validateddiseasemetatranscriptomicsmicrobiomepopulation studiessaliva |
| spellingShingle | Ryan Toma Ying Cai Oyetunji Ogundijo Lan Hu Stephanie Gline Diana Demusaj Nathan Duval Pedro Torres Francine Camacho Guruduth Banavar Momchilo Vuyisich A clinically validated human saliva metatranscriptomic test for global systems biology studies BioTechniques clinically validated disease metatranscriptomics microbiome population studies saliva |
| title | A clinically validated human saliva metatranscriptomic test for global systems biology studies |
| title_full | A clinically validated human saliva metatranscriptomic test for global systems biology studies |
| title_fullStr | A clinically validated human saliva metatranscriptomic test for global systems biology studies |
| title_full_unstemmed | A clinically validated human saliva metatranscriptomic test for global systems biology studies |
| title_short | A clinically validated human saliva metatranscriptomic test for global systems biology studies |
| title_sort | clinically validated human saliva metatranscriptomic test for global systems biology studies |
| topic | clinically validated disease metatranscriptomics microbiome population studies saliva |
| url | https://www.future-science.com/doi/10.2144/btn-2022-0104 |
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